Enteric salmonellosis disrupts the microbial ecology of the murine gastrointestinal tract

Melissa Barman, David Unold, Kathleen Shifley, Elad Amir, Kueichun Hung, Nicolaas Bos, Nita Salzman

Research output: Contribution to journalArticle

237 Citations (Scopus)

Abstract

The commensal microbiota protects the murine host from enteric pathogens. Nevertheless, specific pathogens are able to colonize the intestinal tract and invade, despite the presence of an intact biota. Possibly, effective pathogens disrupt the indigenous microbiota, either directly through pathogen-commensal interaction, indirectly via the host mucosal immune response to the pathogen, or by a combination of these factors. This study investigates the effect of peroral Salmonella enterica serovar Typhimurium infection on the intestinal microbiota. Since the majority of the intestinal microbiota cannot be cultured by conventional techniques, molecular approaches using 16S rRNA sequences were applied. Several major bacterial groups were assayed using quantitative PCR. Administration of either the 50% lethal dose (LD50) or 10x LD 50 of Salmonella enterica serovar Typhimurium caused changes in the microbiota throughout the intestinal tract over the time course of infection. A 95% decrease in total bacterial number was noted in the cecum and large intestine with 10x LD50 S. enterica serovar Typhimurium challenge at 7 days postinfection, concurrent with gross evidence of diarrhea. In addition, alterations in microbiota composition preceded the onset of diarrhea, suggesting the involvement of pathogen-commensal interactions and/or host responses unrelated to diarrhea. Microbiota alterations were not permanent and reverted to the microbiota of uninfected mice by 1 month postinfection. Infection with a Salmonella pathogenicity island 1 (SPI1) mutant did not result in microbiota alterations, while SPI2 mutant infections triggered partial changes. Neither mutant was capable of prolonged colonization or induction of mucosal inflammation. These data suggest that several Salmonella virulence factors, particularly those involved in the local mucosal host response, are required for disruption of the intestinal ecosystem.

Original languageEnglish (US)
Pages (from-to)907-915
Number of pages9
JournalInfection and Immunity
Volume76
Issue number3
DOIs
StatePublished - Mar 1 2008
Externally publishedYes

Fingerprint

Salmonella Infections
Microbiota
Ecology
Gastrointestinal Tract
Salmonella enterica
Lethal Dose 50
Diarrhea
Infection
Salmonella
Genomic Islands
Biota
Mucosal Immunity
Cecum
Large Intestine
Virulence Factors
Ecosystem
Inflammation
Polymerase Chain Reaction
Serogroup
Gastrointestinal Microbiome

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Enteric salmonellosis disrupts the microbial ecology of the murine gastrointestinal tract. / Barman, Melissa; Unold, David; Shifley, Kathleen; Amir, Elad; Hung, Kueichun; Bos, Nicolaas; Salzman, Nita.

In: Infection and Immunity, Vol. 76, No. 3, 01.03.2008, p. 907-915.

Research output: Contribution to journalArticle

Barman, Melissa ; Unold, David ; Shifley, Kathleen ; Amir, Elad ; Hung, Kueichun ; Bos, Nicolaas ; Salzman, Nita. / Enteric salmonellosis disrupts the microbial ecology of the murine gastrointestinal tract. In: Infection and Immunity. 2008 ; Vol. 76, No. 3. pp. 907-915.
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