Abstract
Antimicrobial peptides are important effectors of innate immunity throughout the plant and animal kingdoms. In the mammalian small intestine, Paneth cell α-defensins are antimicrobial peptides that contribute to host defense against enteric pathogens. To determine if α-defensins also govern intestinal microbial ecology, we analyzed the intestinal microbiota of mice expressing a human α-defensin gene (DEFA5) and in mice lacking an enzyme required for the processing of mouse α-defensins. In these complementary models, we detected significant α-defensin-dependent changes in microbiota composition, but not in total bacterial numbers. Furthermore, DEFA5-expressing mice had striking losses of segmented filamentous bacteria and fewer interleukin 17 (IL-17)-producing lamina propria T cells. Our data ascribe a new homeostatic role to α-defensins in regulating the makeup of the commensal microbiota.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 76-83 |
| Number of pages | 8 |
| Journal | Nature Immunology |
| Volume | 11 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2010 |
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ASJC Scopus subject areas
- Immunology
Cite this
Enteric defensins are essential regulators of intestinal microbial ecology. / Salzman, Nita H.; Hung, Kuiechun; Haribhai, Dipica; Chu, Hiutung; Karlsson-Sjöberg, Jenny; Amir, Elad; Teggatz, Paul; Barman, Melissa; Hayward, Michael; Eastwood, Daniel; Stoel, Maaike; Zhou, Yanjiao; Sodergren, Erica; Weinstock, George M.; Bevins, Charles L; Williams, Calvin B.; Bos, Nicolaas A.
In: Nature Immunology, Vol. 11, No. 1, 01.2010, p. 76-83.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enteric defensins are essential regulators of intestinal microbial ecology
AU - Salzman, Nita H.
AU - Hung, Kuiechun
AU - Haribhai, Dipica
AU - Chu, Hiutung
AU - Karlsson-Sjöberg, Jenny
AU - Amir, Elad
AU - Teggatz, Paul
AU - Barman, Melissa
AU - Hayward, Michael
AU - Eastwood, Daniel
AU - Stoel, Maaike
AU - Zhou, Yanjiao
AU - Sodergren, Erica
AU - Weinstock, George M.
AU - Bevins, Charles L
AU - Williams, Calvin B.
AU - Bos, Nicolaas A.
PY - 2010/1
Y1 - 2010/1
N2 - Antimicrobial peptides are important effectors of innate immunity throughout the plant and animal kingdoms. In the mammalian small intestine, Paneth cell α-defensins are antimicrobial peptides that contribute to host defense against enteric pathogens. To determine if α-defensins also govern intestinal microbial ecology, we analyzed the intestinal microbiota of mice expressing a human α-defensin gene (DEFA5) and in mice lacking an enzyme required for the processing of mouse α-defensins. In these complementary models, we detected significant α-defensin-dependent changes in microbiota composition, but not in total bacterial numbers. Furthermore, DEFA5-expressing mice had striking losses of segmented filamentous bacteria and fewer interleukin 17 (IL-17)-producing lamina propria T cells. Our data ascribe a new homeostatic role to α-defensins in regulating the makeup of the commensal microbiota.
AB - Antimicrobial peptides are important effectors of innate immunity throughout the plant and animal kingdoms. In the mammalian small intestine, Paneth cell α-defensins are antimicrobial peptides that contribute to host defense against enteric pathogens. To determine if α-defensins also govern intestinal microbial ecology, we analyzed the intestinal microbiota of mice expressing a human α-defensin gene (DEFA5) and in mice lacking an enzyme required for the processing of mouse α-defensins. In these complementary models, we detected significant α-defensin-dependent changes in microbiota composition, but not in total bacterial numbers. Furthermore, DEFA5-expressing mice had striking losses of segmented filamentous bacteria and fewer interleukin 17 (IL-17)-producing lamina propria T cells. Our data ascribe a new homeostatic role to α-defensins in regulating the makeup of the commensal microbiota.
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UR - http://www.scopus.com/inward/citedby.url?scp=74049122536&partnerID=8YFLogxK
U2 - 10.1038/ni.1825
DO - 10.1038/ni.1825
M3 - Article
C2 - 19855381
AN - SCOPUS:74049122536
VL - 11
SP - 76
EP - 83
JO - Nature Immunology
JF - Nature Immunology
SN - 1529-2908
IS - 1
ER -