Enrichment of Schwann cell cultures from neonatal rat sciatic nerve by differential adhesion

B. Q. Kreider, A. Messing, H. Doan, S. U. Kim, R. P. Lisak, David E Pleasure

Research output: Contribution to journalArticle

115 Scopus citations

Abstract

A novel method of Schwann cell purification from neonatal rat sciatic nerve has been developed using differential adhesion. After enzymatic and mechanical dissociation, the cell digest is allowed to settle on polylysine-coated glass coverslips for 30 min with intermittent shaking. After an 18-h incubation, bipolar cells comprise > 95% of the non-adherent population. Indirect immunofluorescence with the cell-specific markers rabbit anti-galactocerebroside and rabbit anti-bovinr-P-2 basic protein antiserum confirmed light microscopic identification of these bipolar cells as Schwann cells. Rabbit anti-human fibronectin specifically labeled fibroblasts which comprised < 5% of the cell population, but did not bind to Schwann cells. Schwann cells isolated by differential adhesion were injected into a rabbit. When absorbed with cultured rat skin fibroblasts, serum from this rabbit specifically surface labeled > 99% of the bipolar and round cells after 18 h and 5 days in vitro and also labeled Schwann cells in fetal rat dorsal root ganglia cultures, but not fibroblasts or neurons.

Original languageEnglish (US)
Pages (from-to)433-444
Number of pages12
JournalBrain Research
Volume207
Issue number2
DOIs
StatePublished - Mar 2 1981
Externally publishedYes

Keywords

  • differential adhesion
  • galactocerebroside
  • P-2 basic protein
  • Schwann cell

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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