Enhancing heterologous protection in pigs vaccinated with chimeric porcine reproductive and respiratory syndrome virus containing the full-length sequences of shuffled structural genes of multiple heterologous strains

Debin Tian, Dianjun Cao, C. Lynn Heffron, Danielle M. Yugo, Adam J. Rogers, Christopher Overend, Shannon R. Matzinger, Sakthivel Subramaniam, Tanja Opriessnig, Tanya LeRoith, Xiang Jin Meng

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of arguably the most economically important global swine disease. The extensive genetic variation of PRRSV strains is a major obstacle for heterologous protection of current vaccines. Previously, we constructed a panel of chimeric viruses containing only the ectodomain sequences of DNA-shuffled structural genes of different PRRSV strains in the backbone of a commercial vaccine, and found that one chimeric virus had an improved cross-protection efficacy. In this present study, to further enhance the cross-protective efficacy against heterologous strains, we constructed a novel chimeric virus VR2385-S3456 containing the full-length sequences of shuffled structural genes (ORFs 3-6) from 6 heterologous PRRSV strains in the backbone of PRRSV strain VR2385. We showed that the chimeric virus VR2385-S3456 induced a high level of neutralizing antibodies in pigs against two heterologous strains. A subsequent vaccination and challenge study in 48 pigs revealed that the chimeric virus VR2385-S3456 conferred an enhanced cross-protection when challenged with heterologous virus strain NADC20 or a contemporary heterologous strain RFLP 1-7-4. The results suggest that the chimera VR2385-S3456 may be a good PRRSV vaccine candidate for further development to confer heterologous protection.

Original languageEnglish (US)
Pages (from-to)2427-2434
Number of pages8
JournalVaccine
Volume35
Issue number18
DOIs
StatePublished - Apr 25 2017

Fingerprint

Porcine respiratory and reproductive syndrome virus
Porcine reproductive and respiratory syndrome virus
structural genes
Swine
Viruses
swine
viruses
Cross Protection
Genes
Vaccines
vaccines
Swine Diseases
Neutralizing Antibodies
Restriction Fragment Length Polymorphisms
swine diseases
Open Reading Frames
chimerism
Vaccination
neutralizing antibodies
open reading frames

Keywords

  • Cross-protection
  • DNA shuffling
  • Heterologous strains
  • Porcine reproductive and respiratory syndrome virus (PRRSV)
  • RFLP 1-7-4
  • Vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Enhancing heterologous protection in pigs vaccinated with chimeric porcine reproductive and respiratory syndrome virus containing the full-length sequences of shuffled structural genes of multiple heterologous strains. / Tian, Debin; Cao, Dianjun; Lynn Heffron, C.; Yugo, Danielle M.; Rogers, Adam J.; Overend, Christopher; Matzinger, Shannon R.; Subramaniam, Sakthivel; Opriessnig, Tanja; LeRoith, Tanya; Meng, Xiang Jin.

In: Vaccine, Vol. 35, No. 18, 25.04.2017, p. 2427-2434.

Research output: Contribution to journalArticle

Tian, D, Cao, D, Lynn Heffron, C, Yugo, DM, Rogers, AJ, Overend, C, Matzinger, SR, Subramaniam, S, Opriessnig, T, LeRoith, T & Meng, XJ 2017, 'Enhancing heterologous protection in pigs vaccinated with chimeric porcine reproductive and respiratory syndrome virus containing the full-length sequences of shuffled structural genes of multiple heterologous strains', Vaccine, vol. 35, no. 18, pp. 2427-2434. https://doi.org/10.1016/j.vaccine.2017.03.046
Tian, Debin ; Cao, Dianjun ; Lynn Heffron, C. ; Yugo, Danielle M. ; Rogers, Adam J. ; Overend, Christopher ; Matzinger, Shannon R. ; Subramaniam, Sakthivel ; Opriessnig, Tanja ; LeRoith, Tanya ; Meng, Xiang Jin. / Enhancing heterologous protection in pigs vaccinated with chimeric porcine reproductive and respiratory syndrome virus containing the full-length sequences of shuffled structural genes of multiple heterologous strains. In: Vaccine. 2017 ; Vol. 35, No. 18. pp. 2427-2434.
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