Abstract
Background: Genetic variation in monoamine oxidase A (MAOA) and serotonin transporter (5-HTT)-linked polymorphic regions (LPR) is associated with neuropsychiatric behavior. Methods: We genotyped 37 macaques using conventional PCR product gel fractionation and by capillary electrophoresis of multiplexed amplicons and compared the data. Results: Genotype concordance was 97% and 95% for MAOA-LPR and 5-HTT-LPR, respectively. Capillary electrophoresis was more sensitive and cost-effective. Conclusions: Multiplexing MAOA-LPR and 5-HTT-LPR will enhance the genotyping of large sample sets.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 407-411 |
| Number of pages | 5 |
| Journal | Journal of Medical Primatology |
| Volume | 41 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 2012 |
Keywords
- Capillary electrophoresis
- Monoamine oxidase A
- Multiplexing
- Rhesus macaque
- serotonin transporter
ASJC Scopus subject areas
- Animal Science and Zoology
- veterinary(all)
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Karere, G. M., Sullivan, E., Kinnally, E. L., Capitanio, J. P., & Lyons, L. A. (2012). Enhancing genotyping of MAOA-LPR and 5-HTT-LPR in rhesus macaques (Macaca mulatta). Journal of Medical Primatology, 41(6), 407-411. https://doi.org/10.1111/jmp.12024