Abstract
Background: Genetic variation in monoamine oxidase A (MAOA) and serotonin transporter (5-HTT)-linked polymorphic regions (LPR) is associated with neuropsychiatric behavior. Methods: We genotyped 37 macaques using conventional PCR product gel fractionation and by capillary electrophoresis of multiplexed amplicons and compared the data. Results: Genotype concordance was 97% and 95% for MAOA-LPR and 5-HTT-LPR, respectively. Capillary electrophoresis was more sensitive and cost-effective. Conclusions: Multiplexing MAOA-LPR and 5-HTT-LPR will enhance the genotyping of large sample sets.
Original language | English (US) |
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Pages (from-to) | 407-411 |
Number of pages | 5 |
Journal | Journal of Medical Primatology |
Volume | 41 |
Issue number | 6 |
DOIs | |
State | Published - Dec 2012 |
Keywords
- Capillary electrophoresis
- Monoamine oxidase A
- Multiplexing
- Rhesus macaque
- serotonin transporter
ASJC Scopus subject areas
- Animal Science and Zoology
- veterinary(all)