Enhancing genotyping of MAOA-LPR and 5-HTT-LPR in rhesus macaques (Macaca mulatta)

Genesio M. Karere, Erin Sullivan, Erin L. Kinnally, John P. Capitanio, Leslie A Lyons

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Background: Genetic variation in monoamine oxidase A (MAOA) and serotonin transporter (5-HTT)-linked polymorphic regions (LPR) is associated with neuropsychiatric behavior. Methods: We genotyped 37 macaques using conventional PCR product gel fractionation and by capillary electrophoresis of multiplexed amplicons and compared the data. Results: Genotype concordance was 97% and 95% for MAOA-LPR and 5-HTT-LPR, respectively. Capillary electrophoresis was more sensitive and cost-effective. Conclusions: Multiplexing MAOA-LPR and 5-HTT-LPR will enhance the genotyping of large sample sets.

Original languageEnglish (US)
Pages (from-to)407-411
Number of pages5
JournalJournal of Medical Primatology
Volume41
Issue number6
DOIs
StatePublished - Dec 2012

Keywords

  • Capillary electrophoresis
  • Monoamine oxidase A
  • Multiplexing
  • Rhesus macaque
  • serotonin transporter

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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