Abstract
Background: Genetic variation in monoamine oxidase A (MAOA) and serotonin transporter (5-HTT)-linked polymorphic regions (LPR) is associated with neuropsychiatric behavior. Methods: We genotyped 37 macaques using conventional PCR product gel fractionation and by capillary electrophoresis of multiplexed amplicons and compared the data. Results: Genotype concordance was 97% and 95% for MAOA-LPR and 5-HTT-LPR, respectively. Capillary electrophoresis was more sensitive and cost-effective. Conclusions: Multiplexing MAOA-LPR and 5-HTT-LPR will enhance the genotyping of large sample sets.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 407-411 |
| Number of pages | 5 |
| Journal | Journal of Medical Primatology |
| Volume | 41 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 2012 |
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Keywords
- Capillary electrophoresis
- Monoamine oxidase A
- Multiplexing
- Rhesus macaque
- serotonin transporter
ASJC Scopus subject areas
- Animal Science and Zoology
- veterinary(all)
Cite this
Enhancing genotyping of MAOA-LPR and 5-HTT-LPR in rhesus macaques (Macaca mulatta). / Karere, Genesio M.; Sullivan, Erin; Kinnally, Erin L.; Capitanio, John P.; Lyons, Leslie A.
In: Journal of Medical Primatology, Vol. 41, No. 6, 12.2012, p. 407-411.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhancing genotyping of MAOA-LPR and 5-HTT-LPR in rhesus macaques (Macaca mulatta)
AU - Karere, Genesio M.
AU - Sullivan, Erin
AU - Kinnally, Erin L.
AU - Capitanio, John P.
AU - Lyons, Leslie A
PY - 2012/12
Y1 - 2012/12
N2 - Background: Genetic variation in monoamine oxidase A (MAOA) and serotonin transporter (5-HTT)-linked polymorphic regions (LPR) is associated with neuropsychiatric behavior. Methods: We genotyped 37 macaques using conventional PCR product gel fractionation and by capillary electrophoresis of multiplexed amplicons and compared the data. Results: Genotype concordance was 97% and 95% for MAOA-LPR and 5-HTT-LPR, respectively. Capillary electrophoresis was more sensitive and cost-effective. Conclusions: Multiplexing MAOA-LPR and 5-HTT-LPR will enhance the genotyping of large sample sets.
AB - Background: Genetic variation in monoamine oxidase A (MAOA) and serotonin transporter (5-HTT)-linked polymorphic regions (LPR) is associated with neuropsychiatric behavior. Methods: We genotyped 37 macaques using conventional PCR product gel fractionation and by capillary electrophoresis of multiplexed amplicons and compared the data. Results: Genotype concordance was 97% and 95% for MAOA-LPR and 5-HTT-LPR, respectively. Capillary electrophoresis was more sensitive and cost-effective. Conclusions: Multiplexing MAOA-LPR and 5-HTT-LPR will enhance the genotyping of large sample sets.
KW - Capillary electrophoresis
KW - Monoamine oxidase A
KW - Multiplexing
KW - Rhesus macaque
KW - serotonin transporter
UR - http://www.scopus.com/inward/record.url?scp=84868678430&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84868678430&partnerID=8YFLogxK
U2 - 10.1111/jmp.12024
DO - 10.1111/jmp.12024
M3 - Article
C2 - 23078595
AN - SCOPUS:84868678430
VL - 41
SP - 407
EP - 411
JO - Journal of Medical Primatology
JF - Journal of Medical Primatology
SN - 0047-2565
IS - 6
ER -