Enhancing genotyping of MAOA-LPR and 5-HTT-LPR in rhesus macaques (Macaca mulatta)

Genesio M. Karere, Erin Sullivan, Erin L. Kinnally, John P. Capitanio, Leslie A Lyons

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Genetic variation in monoamine oxidase A (MAOA) and serotonin transporter (5-HTT)-linked polymorphic regions (LPR) is associated with neuropsychiatric behavior. Methods: We genotyped 37 macaques using conventional PCR product gel fractionation and by capillary electrophoresis of multiplexed amplicons and compared the data. Results: Genotype concordance was 97% and 95% for MAOA-LPR and 5-HTT-LPR, respectively. Capillary electrophoresis was more sensitive and cost-effective. Conclusions: Multiplexing MAOA-LPR and 5-HTT-LPR will enhance the genotyping of large sample sets.

Original languageEnglish (US)
Pages (from-to)407-411
Number of pages5
JournalJournal of Medical Primatology
Volume41
Issue number6
DOIs
StatePublished - Dec 2012

Fingerprint

amine oxidase (flavin-containing)
Monoamine Oxidase
Macaca mulatta
genotyping
capillary electrophoresis
Capillary Electrophoresis
Serotonin Plasma Membrane Transport Proteins
Macaca
serotonin
transporters
fractionation
Gels
Genotype
gels
Costs and Cost Analysis
Polymerase Chain Reaction
genetic variation
genotype
sampling
methodology

Keywords

  • Capillary electrophoresis
  • Monoamine oxidase A
  • Multiplexing
  • Rhesus macaque
  • serotonin transporter

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Enhancing genotyping of MAOA-LPR and 5-HTT-LPR in rhesus macaques (Macaca mulatta). / Karere, Genesio M.; Sullivan, Erin; Kinnally, Erin L.; Capitanio, John P.; Lyons, Leslie A.

In: Journal of Medical Primatology, Vol. 41, No. 6, 12.2012, p. 407-411.

Research output: Contribution to journalArticle

Karere, Genesio M. ; Sullivan, Erin ; Kinnally, Erin L. ; Capitanio, John P. ; Lyons, Leslie A. / Enhancing genotyping of MAOA-LPR and 5-HTT-LPR in rhesus macaques (Macaca mulatta). In: Journal of Medical Primatology. 2012 ; Vol. 41, No. 6. pp. 407-411.
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