Enhancing genotyping of MAOA-LPR and 5-HTT-LPR in rhesus macaques (Macaca mulatta)

Genesio M. Karere; Erin Sullivan; Erin L. Kinnally; John P. Capitanio; Leslie A Lyons

doi:10.1111/jmp.12024

Enhancing genotyping of MAOA-LPR and 5-HTT-LPR in rhesus macaques (Macaca mulatta)

Genesio M. Karere, Erin Sullivan, Erin L. Kinnally, John P. Capitanio, Leslie A Lyons

Population Health and Reproduction

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Background: Genetic variation in monoamine oxidase A (MAOA) and serotonin transporter (5-HTT)-linked polymorphic regions (LPR) is associated with neuropsychiatric behavior. Methods: We genotyped 37 macaques using conventional PCR product gel fractionation and by capillary electrophoresis of multiplexed amplicons and compared the data. Results: Genotype concordance was 97% and 95% for MAOA-LPR and 5-HTT-LPR, respectively. Capillary electrophoresis was more sensitive and cost-effective. Conclusions: Multiplexing MAOA-LPR and 5-HTT-LPR will enhance the genotyping of large sample sets.

Original language	English (US)
Pages (from-to)	407-411
Number of pages	5
Journal	Journal of Medical Primatology
Volume	41
Issue number	6
DOIs	https://doi.org/10.1111/jmp.12024
State	Published - Dec 2012

Keywords

Capillary electrophoresis
Monoamine oxidase A
Multiplexing
Rhesus macaque
serotonin transporter

ASJC Scopus subject areas

Animal Science and Zoology
veterinary(all)

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10.1111/jmp.12024

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title = "Enhancing genotyping of MAOA-LPR and 5-HTT-LPR in rhesus macaques (Macaca mulatta)",

abstract = "Background: Genetic variation in monoamine oxidase A (MAOA) and serotonin transporter (5-HTT)-linked polymorphic regions (LPR) is associated with neuropsychiatric behavior. Methods: We genotyped 37 macaques using conventional PCR product gel fractionation and by capillary electrophoresis of multiplexed amplicons and compared the data. Results: Genotype concordance was 97% and 95% for MAOA-LPR and 5-HTT-LPR, respectively. Capillary electrophoresis was more sensitive and cost-effective. Conclusions: Multiplexing MAOA-LPR and 5-HTT-LPR will enhance the genotyping of large sample sets.",

keywords = "Capillary electrophoresis, Monoamine oxidase A, Multiplexing, Rhesus macaque, serotonin transporter",

author = "Karere, {Genesio M.} and Erin Sullivan and Kinnally, {Erin L.} and Capitanio, {John P.} and Lyons, {Leslie A}",

year = "2012",

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language = "English (US)",

volume = "41",

pages = "407--411",

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publisher = "Blackwell Munksgaard",

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TY - JOUR

T1 - Enhancing genotyping of MAOA-LPR and 5-HTT-LPR in rhesus macaques (Macaca mulatta)

AU - Karere, Genesio M.

AU - Sullivan, Erin

AU - Kinnally, Erin L.

AU - Capitanio, John P.

AU - Lyons, Leslie A

PY - 2012/12

Y1 - 2012/12

N2 - Background: Genetic variation in monoamine oxidase A (MAOA) and serotonin transporter (5-HTT)-linked polymorphic regions (LPR) is associated with neuropsychiatric behavior. Methods: We genotyped 37 macaques using conventional PCR product gel fractionation and by capillary electrophoresis of multiplexed amplicons and compared the data. Results: Genotype concordance was 97% and 95% for MAOA-LPR and 5-HTT-LPR, respectively. Capillary electrophoresis was more sensitive and cost-effective. Conclusions: Multiplexing MAOA-LPR and 5-HTT-LPR will enhance the genotyping of large sample sets.

AB - Background: Genetic variation in monoamine oxidase A (MAOA) and serotonin transporter (5-HTT)-linked polymorphic regions (LPR) is associated with neuropsychiatric behavior. Methods: We genotyped 37 macaques using conventional PCR product gel fractionation and by capillary electrophoresis of multiplexed amplicons and compared the data. Results: Genotype concordance was 97% and 95% for MAOA-LPR and 5-HTT-LPR, respectively. Capillary electrophoresis was more sensitive and cost-effective. Conclusions: Multiplexing MAOA-LPR and 5-HTT-LPR will enhance the genotyping of large sample sets.

KW - Capillary electrophoresis

KW - Monoamine oxidase A

KW - Multiplexing

KW - Rhesus macaque

KW - serotonin transporter

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Enhancing genotyping of MAOA-LPR and 5-HTT-LPR in rhesus macaques (Macaca mulatta)

Abstract

Keywords

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