Enhancement of embryonic stem cell differentiation promoted by avian chorioallantoic membranes

Dodanim Talavera-Adame, Donald C. Dafoe, Tina T. Ng, Sebastian Wachsmann-Hogiu, Carlos Castillo-Henkel, Daniel L. Farkas

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Avian chorioallantoic membrane (CAM) has been used as a model to explore angiogenesis and to study the microvasculature of transplanted tissues. Because CAM provides a vascular bed, cells can be implanted, and their development can be monitored and modified. We used the CAM model to study the differentiation process of embryoid bodies (EBs) derived from mouse embryonic stem cells (ESCs) influenced by the CAM vascular bed. After EBs were incubated in CAM for 5 days, they underwent further differentiation and became tissue masses (TMs) of different morphologies from those that grew outside CAM. Immunohistochemical analysis of TMs demonstrated tissue-specific markers such as neurofilament light, CD34, collagen IV, cardiac myosin heavy chain (MHC), and cardiotin. Differentiated mouse blood vessels stained with anti-CD31 were found within the TMs, as well as blood vessels stained positive for QH1 and QCPN, markers for quail endothelial cells and perinuclear quail antigen, respectively. Quail erythrocytes inside mouse blood vessels suggested a connection between existing quail vessels and blood vessels growing inside the TMs as a result of EB differentiation. Therefore, CAM could be a suitable model to trigger and study the differentiation of EBs in close interaction with surrogate quail blood vessels.

Original languageEnglish (US)
Pages (from-to)3193-3200
Number of pages8
JournalTissue Engineering - Part A
Volume15
Issue number10
DOIs
StatePublished - Oct 1 2009

Fingerprint

Chorioallantoic Membrane
Embryonic Stem Cells
Stem cells
Blood Vessels
Quail
Cell Differentiation
Blood vessels
Embryoid Bodies
Membranes
Tissue
Cardiac Myosins
Intermediate Filaments
Myosin Heavy Chains
Endothelial cells
Antigens
Microvessels
Collagen
Endothelial Cells
Erythrocytes
Light

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials
  • Medicine(all)

Cite this

Enhancement of embryonic stem cell differentiation promoted by avian chorioallantoic membranes. / Talavera-Adame, Dodanim; Dafoe, Donald C.; Ng, Tina T.; Wachsmann-Hogiu, Sebastian; Castillo-Henkel, Carlos; Farkas, Daniel L.

In: Tissue Engineering - Part A, Vol. 15, No. 10, 01.10.2009, p. 3193-3200.

Research output: Contribution to journalArticle

Talavera-Adame, Dodanim ; Dafoe, Donald C. ; Ng, Tina T. ; Wachsmann-Hogiu, Sebastian ; Castillo-Henkel, Carlos ; Farkas, Daniel L. / Enhancement of embryonic stem cell differentiation promoted by avian chorioallantoic membranes. In: Tissue Engineering - Part A. 2009 ; Vol. 15, No. 10. pp. 3193-3200.
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