Enhancement of antigen-specific CD4+ and CD8+ T cell responses using a self-assembled biologic nanolipoprotein particle vaccine

Dina Weilhammer, Alexis D. Dunkle, Craig D. Blanchette, Nicholas O. Fischer, Michele Corzett, Doerte Lehmann, Tyler Boone, Paul D Hoeprich, Adam Driks, Amy Rasley

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

To address the need for vaccine platforms that induce robust cell-mediated immunity, we investigated the potential of utilizing self-assembling biologic nanolipoprotein particles (NLPs) as an antigen and adjuvant delivery system to induce antigen-specific murine T cell responses. We utilized OT-I and OT-II TCR-transgenic mice to investigate the effects of NLP-mediated delivery of the model antigen ovalbumin (OVA) on T cell activation. Delivery of OVA with the TLR4 agonist monophosphoryl lipid A (MPLA) in the context of NLPs significantly enhanced the activation of both CD4+ and CD8+ T cells in vitro compared to co-administration of free OVA and MPLA. Upon intranasal immunization of mice harboring TCR-transgenic cells, NLPs enhanced the adjuvant effects of MPLA and the in vivo delivery of OVA, leading to significantly increased expansion of CD4+ and CD8+ T cells in lung-draining lymph nodes. Therefore, NLPs are a promising vaccine platform for inducing T cell responses following intranasal administration.

Original languageEnglish (US)
Pages (from-to)1475-1481
Number of pages7
JournalVaccine
Volume35
Issue number11
DOIs
StatePublished - Mar 13 2017
Externally publishedYes

Keywords

  • Intranasal
  • Nanodisc
  • Nanolipoprotein
  • Nanoparticle
  • T cell activation
  • Vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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    Weilhammer, D., Dunkle, A. D., Blanchette, C. D., Fischer, N. O., Corzett, M., Lehmann, D., Boone, T., Hoeprich, P. D., Driks, A., & Rasley, A. (2017). Enhancement of antigen-specific CD4+ and CD8+ T cell responses using a self-assembled biologic nanolipoprotein particle vaccine. Vaccine, 35(11), 1475-1481. https://doi.org/10.1016/j.vaccine.2017.02.004