Enhanced recovery of avian influenza virus isolates by a combination of chicken embryo inoculation methods

P. R. Woolcock, M. D. McFarland, S. Lai, Richard P Chin

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Since 1993, 14 cases of avian influenza from four different states in the U.S.A. have been diagnosed by virus isolation from eight avian species. Only 11 of the 14 avian influenza virus (AIV) primary isolations would have been successful if only the standard protocol for AIV isolation, i.e., inoculation of specific-pathogen-free embryonating chicken eggs (ECEs) by the chorioallantoic sac (CAS) route, had been followed. Primary isolation attempts were negative for AIV in three cases in which ECEs were inoculated by the CAS route; AIV could not be detected by hemagglutinating activity, agar gel immunodiffusion test or negative stain electron microscopy. However, in these three cases, primary isolations of AIV were achieved by inoculation of ECEs into either the yolk sac or onto the chorioallantoic membrane.

Original languageEnglish (US)
Pages (from-to)1030-1035
Number of pages6
JournalAvian Diseases
Volume45
Issue number4
StatePublished - 2001

Keywords

  • Avian influenza
  • Virus identification
  • Virus isolation

ASJC Scopus subject areas

  • Immunology
  • Cancer Research
  • veterinary(all)

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