Abstract
Since 1993, 14 cases of avian influenza from four different states in the U.S.A. have been diagnosed by virus isolation from eight avian species. Only 11 of the 14 avian influenza virus (AIV) primary isolations would have been successful if only the standard protocol for AIV isolation, i.e., inoculation of specific-pathogen-free embryonating chicken eggs (ECEs) by the chorioallantoic sac (CAS) route, had been followed. Primary isolation attempts were negative for AIV in three cases in which ECEs were inoculated by the CAS route; AIV could not be detected by hemagglutinating activity, agar gel immunodiffusion test or negative stain electron microscopy. However, in these three cases, primary isolations of AIV were achieved by inoculation of ECEs into either the yolk sac or onto the chorioallantoic membrane.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1030-1035 |
| Number of pages | 6 |
| Journal | Avian Diseases |
| Volume | 45 |
| Issue number | 4 |
| State | Published - 2001 |
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Keywords
- Avian influenza
- Virus identification
- Virus isolation
ASJC Scopus subject areas
- Immunology
- Cancer Research
- veterinary(all)
Cite this
Enhanced recovery of avian influenza virus isolates by a combination of chicken embryo inoculation methods. / Woolcock, P. R.; McFarland, M. D.; Lai, S.; Chin, Richard P.
In: Avian Diseases, Vol. 45, No. 4, 2001, p. 1030-1035.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhanced recovery of avian influenza virus isolates by a combination of chicken embryo inoculation methods
AU - Woolcock, P. R.
AU - McFarland, M. D.
AU - Lai, S.
AU - Chin, Richard P
PY - 2001
Y1 - 2001
N2 - Since 1993, 14 cases of avian influenza from four different states in the U.S.A. have been diagnosed by virus isolation from eight avian species. Only 11 of the 14 avian influenza virus (AIV) primary isolations would have been successful if only the standard protocol for AIV isolation, i.e., inoculation of specific-pathogen-free embryonating chicken eggs (ECEs) by the chorioallantoic sac (CAS) route, had been followed. Primary isolation attempts were negative for AIV in three cases in which ECEs were inoculated by the CAS route; AIV could not be detected by hemagglutinating activity, agar gel immunodiffusion test or negative stain electron microscopy. However, in these three cases, primary isolations of AIV were achieved by inoculation of ECEs into either the yolk sac or onto the chorioallantoic membrane.
AB - Since 1993, 14 cases of avian influenza from four different states in the U.S.A. have been diagnosed by virus isolation from eight avian species. Only 11 of the 14 avian influenza virus (AIV) primary isolations would have been successful if only the standard protocol for AIV isolation, i.e., inoculation of specific-pathogen-free embryonating chicken eggs (ECEs) by the chorioallantoic sac (CAS) route, had been followed. Primary isolation attempts were negative for AIV in three cases in which ECEs were inoculated by the CAS route; AIV could not be detected by hemagglutinating activity, agar gel immunodiffusion test or negative stain electron microscopy. However, in these three cases, primary isolations of AIV were achieved by inoculation of ECEs into either the yolk sac or onto the chorioallantoic membrane.
KW - Avian influenza
KW - Virus identification
KW - Virus isolation
UR - http://www.scopus.com/inward/record.url?scp=0035544245&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035544245&partnerID=8YFLogxK
M3 - Article
C2 - 11785874
AN - SCOPUS:0035544245
VL - 45
SP - 1030
EP - 1035
JO - Avian Diseases
JF - Avian Diseases
SN - 0005-2086
IS - 4
ER -