Enhanced radiation response in radioresistant MCF-7 cells by targeting peroxiredoxin II

Anthony Joseph Gomez Diaz, Daniel Tamae, Yun Yen, Jian-Jian Li, Tieli Wang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In our previous study, we identified that a protein target, peroxiredoxin II (PrxII), is overexpressed in radioresistant MCF+FIR3 breast-cancer cells and found that its expression and function is associated with breast-cancer radiation sensitivity or resistance. Small interference RNA (siRNA) targeting PrxII gene expression was able to sensitize MCF+FIR3 radioresistant breast-cancer cells to ionizing radiation. The major focus of this work was to investigate how the radiation response of MCF+FIR3 radioresistant cells was affected by the siRNA that inhibits PrxII gene expression. Our results, presented here, show that silencing PrxII gene expression increased cellular toxicity by altering cellular thiol status, inhibiting Ca2+ efflux from the cells, and perturbing the intracellular Ca2+ homeostasis. By combining radiotherapy and siRNA technology, we hope to develop new therapeutic strategies that may have potential to enhance the efficacy of chemotherapeutic agents due to this technology's property of targeting to specific cancer-related genes.

Original languageEnglish (US)
Pages (from-to)87-101
Number of pages15
JournalBreast Cancer: Targets and Therapy
Volume5
DOIs
StatePublished - Oct 11 2013

Fingerprint

Peroxiredoxins
MCF-7 Cells
RNA Interference
Radiation
Breast Neoplasms
Gene Expression
Technology
Neoplasm Genes
Radiation Tolerance
Ionizing Radiation
Sulfhydryl Compounds
Homeostasis
Radiotherapy
Proteins

Keywords

  • Ca
  • MCF+FIR3
  • PrxII
  • Radiation resistance
  • siRNA

ASJC Scopus subject areas

  • Oncology

Cite this

Enhanced radiation response in radioresistant MCF-7 cells by targeting peroxiredoxin II. / Diaz, Anthony Joseph Gomez; Tamae, Daniel; Yen, Yun; Li, Jian-Jian; Wang, Tieli.

In: Breast Cancer: Targets and Therapy, Vol. 5, 11.10.2013, p. 87-101.

Research output: Contribution to journalArticle

Diaz, Anthony Joseph Gomez ; Tamae, Daniel ; Yen, Yun ; Li, Jian-Jian ; Wang, Tieli. / Enhanced radiation response in radioresistant MCF-7 cells by targeting peroxiredoxin II. In: Breast Cancer: Targets and Therapy. 2013 ; Vol. 5. pp. 87-101.
@article{4536df08378946658197e63672a00837,
title = "Enhanced radiation response in radioresistant MCF-7 cells by targeting peroxiredoxin II",
abstract = "In our previous study, we identified that a protein target, peroxiredoxin II (PrxII), is overexpressed in radioresistant MCF+FIR3 breast-cancer cells and found that its expression and function is associated with breast-cancer radiation sensitivity or resistance. Small interference RNA (siRNA) targeting PrxII gene expression was able to sensitize MCF+FIR3 radioresistant breast-cancer cells to ionizing radiation. The major focus of this work was to investigate how the radiation response of MCF+FIR3 radioresistant cells was affected by the siRNA that inhibits PrxII gene expression. Our results, presented here, show that silencing PrxII gene expression increased cellular toxicity by altering cellular thiol status, inhibiting Ca2+ efflux from the cells, and perturbing the intracellular Ca2+ homeostasis. By combining radiotherapy and siRNA technology, we hope to develop new therapeutic strategies that may have potential to enhance the efficacy of chemotherapeutic agents due to this technology's property of targeting to specific cancer-related genes.",
keywords = "Ca, MCF+FIR3, PrxII, Radiation resistance, siRNA",
author = "Diaz, {Anthony Joseph Gomez} and Daniel Tamae and Yun Yen and Jian-Jian Li and Tieli Wang",
year = "2013",
month = "10",
day = "11",
doi = "10.2147/BCTT.S51378",
language = "English (US)",
volume = "5",
pages = "87--101",
journal = "Breast Cancer: Targets and Therapy",
issn = "1179-1314",
publisher = "Dove Medical Press Ltd.",

}

TY - JOUR

T1 - Enhanced radiation response in radioresistant MCF-7 cells by targeting peroxiredoxin II

AU - Diaz, Anthony Joseph Gomez

AU - Tamae, Daniel

AU - Yen, Yun

AU - Li, Jian-Jian

AU - Wang, Tieli

PY - 2013/10/11

Y1 - 2013/10/11

N2 - In our previous study, we identified that a protein target, peroxiredoxin II (PrxII), is overexpressed in radioresistant MCF+FIR3 breast-cancer cells and found that its expression and function is associated with breast-cancer radiation sensitivity or resistance. Small interference RNA (siRNA) targeting PrxII gene expression was able to sensitize MCF+FIR3 radioresistant breast-cancer cells to ionizing radiation. The major focus of this work was to investigate how the radiation response of MCF+FIR3 radioresistant cells was affected by the siRNA that inhibits PrxII gene expression. Our results, presented here, show that silencing PrxII gene expression increased cellular toxicity by altering cellular thiol status, inhibiting Ca2+ efflux from the cells, and perturbing the intracellular Ca2+ homeostasis. By combining radiotherapy and siRNA technology, we hope to develop new therapeutic strategies that may have potential to enhance the efficacy of chemotherapeutic agents due to this technology's property of targeting to specific cancer-related genes.

AB - In our previous study, we identified that a protein target, peroxiredoxin II (PrxII), is overexpressed in radioresistant MCF+FIR3 breast-cancer cells and found that its expression and function is associated with breast-cancer radiation sensitivity or resistance. Small interference RNA (siRNA) targeting PrxII gene expression was able to sensitize MCF+FIR3 radioresistant breast-cancer cells to ionizing radiation. The major focus of this work was to investigate how the radiation response of MCF+FIR3 radioresistant cells was affected by the siRNA that inhibits PrxII gene expression. Our results, presented here, show that silencing PrxII gene expression increased cellular toxicity by altering cellular thiol status, inhibiting Ca2+ efflux from the cells, and perturbing the intracellular Ca2+ homeostasis. By combining radiotherapy and siRNA technology, we hope to develop new therapeutic strategies that may have potential to enhance the efficacy of chemotherapeutic agents due to this technology's property of targeting to specific cancer-related genes.

KW - Ca

KW - MCF+FIR3

KW - PrxII

KW - Radiation resistance

KW - siRNA

UR - http://www.scopus.com/inward/record.url?scp=84901489137&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901489137&partnerID=8YFLogxK

U2 - 10.2147/BCTT.S51378

DO - 10.2147/BCTT.S51378

M3 - Article

VL - 5

SP - 87

EP - 101

JO - Breast Cancer: Targets and Therapy

JF - Breast Cancer: Targets and Therapy

SN - 1179-1314

ER -