Enhanced innate antiviral gene expression, IFN-α, and cytolytic responses are predictive of mucosal immune recovery during simian immunodeficiency virus infection

David Verhoeven, Michael D. George, William Hu, Angeline T. Dang, Zeljka McBride, Elizabeth Reay, Monica Macal, Anne Fenton, Sumathi Sankaran-Walters, Satya Dandekar

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The mucosa that lines the respiratory and gastrointestinal (GI) tracts is an important portal of entry for pathogens and provides the first line of innate immune defense against infections. Although an abundance of memory CD4+ T cells at mucosal sites render them highly susceptible to HIV infection, the gut and not the lung experiences severe and sustained CD4+ T cell depletion and tissue disruption. We hypothesized that distinct immune responses in the lung and gut during the primary and chronic stages of viral infection contribute to these differences. Using the SIV model of AIDS, we performed a comparative analysis of the molecular and cellular characteristics of host responses in the gut and lung. Our findings showed that both mucosal compartments harbor similar percentages of memory CD4+ T cells and displayed comparable cytokine (IL-2, IFN-γ, and TNF-α) responses to mitogenic stimulations prior to infection. However, despite similar viral replication and CD4+ T cell depletion during primary SIV infection, CD4+ T cell restoration kinetics in the lung and gut diverged during acute viral infection. The CD4+ T cells rebounded or were preserved in the lung mucosa during chronic viral infection, which correlated with heightened induction of type I IFN signaling molecules and innate viral restriction factors. In contrast, the lack of CD4+ T cell restoration in the gut was associated with dampened immune responses and diminished expression of viral restriction factors. Thus, unique immune mechanisms contribute to the differential response and protection of pulmonary versus GI mucosa and can be leveraged to enhance mucosal recovery. The Journal of Immunology, 2014, 192: 3308-3318.

Original languageEnglish (US)
Pages (from-to)3308-3318
Number of pages11
JournalJournal of Immunology
Volume192
Issue number7
DOIs
StatePublished - Apr 1 2014

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Simian Immunodeficiency Virus
Mucosal Immunity
Virus Diseases
Antiviral Agents
T-Lymphocytes
Gene Expression
Lung
Mucous Membrane
Infection
Allergy and Immunology
Respiratory System
HIV Infections
Interleukin-2
Gastrointestinal Tract
Acquired Immunodeficiency Syndrome
Cytokines

ASJC Scopus subject areas

  • Immunology

Cite this

Enhanced innate antiviral gene expression, IFN-α, and cytolytic responses are predictive of mucosal immune recovery during simian immunodeficiency virus infection. / Verhoeven, David; George, Michael D.; Hu, William; Dang, Angeline T.; McBride, Zeljka; Reay, Elizabeth; Macal, Monica; Fenton, Anne; Sankaran-Walters, Sumathi; Dandekar, Satya.

In: Journal of Immunology, Vol. 192, No. 7, 01.04.2014, p. 3308-3318.

Research output: Contribution to journalArticle

Verhoeven, David ; George, Michael D. ; Hu, William ; Dang, Angeline T. ; McBride, Zeljka ; Reay, Elizabeth ; Macal, Monica ; Fenton, Anne ; Sankaran-Walters, Sumathi ; Dandekar, Satya. / Enhanced innate antiviral gene expression, IFN-α, and cytolytic responses are predictive of mucosal immune recovery during simian immunodeficiency virus infection. In: Journal of Immunology. 2014 ; Vol. 192, No. 7. pp. 3308-3318.
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