Enhanced GBX2 expression stimulates growth of human prostate cancer cells via transcriptional up-regulation of the interleukin 6 gene

Allen C Gao, Wei Lou, John T. Isaacs

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Previous studies demonstrated that the GBX2 homeobox gene is consistently overexpressed in cultured human prostate cancer cell lines. In this study, the human GBX2 cDNA was cloned and a quantitative reverse transcription-PCR method used to demonstrate that GBX2 mRNA expression is enhanced in approximately 70% of human prostate cancer tissues compared with normal human prostate tissues. Purified recombinant GBX2 protein binds specifically to an ATTA motif within the promoter of the interleukin 6 (IL-6) gene. Using an antisense approach, down-regulation of the expression of GBX2 correlated with decreased expression of IL-6 and an inhibition of tumorigenicity of PC3 human prostate cancer cells. In addition, in vitro growth of the antisense clones was partially restored by exogenous addition of recombinant IL-6 protein to the culture media. These data demonstrated that enhanced GBX2 expression results in a stimulation of malignant growth of prostate cancer cells and that part of this stimulation involves up- regulation in the transcription of the IL-6 gene.

Original languageEnglish (US)
Pages (from-to)493-497
Number of pages5
JournalClinical Cancer Research
Volume6
Issue number2
StatePublished - Feb 2000
Externally publishedYes

Fingerprint

Interleukin-6
Prostatic Neoplasms
Up-Regulation
Growth
Genes
Homeobox Genes
Recombinant Proteins
Reverse Transcription
Culture Media
Prostate
Down-Regulation
Complementary DNA
Clone Cells
Cell Line
Polymerase Chain Reaction
Messenger RNA
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Enhanced GBX2 expression stimulates growth of human prostate cancer cells via transcriptional up-regulation of the interleukin 6 gene. / Gao, Allen C; Lou, Wei; Isaacs, John T.

In: Clinical Cancer Research, Vol. 6, No. 2, 02.2000, p. 493-497.

Research output: Contribution to journalArticle

@article{2047ea3b1b8c44f0b2d7da74096c10e0,
title = "Enhanced GBX2 expression stimulates growth of human prostate cancer cells via transcriptional up-regulation of the interleukin 6 gene",
abstract = "Previous studies demonstrated that the GBX2 homeobox gene is consistently overexpressed in cultured human prostate cancer cell lines. In this study, the human GBX2 cDNA was cloned and a quantitative reverse transcription-PCR method used to demonstrate that GBX2 mRNA expression is enhanced in approximately 70{\%} of human prostate cancer tissues compared with normal human prostate tissues. Purified recombinant GBX2 protein binds specifically to an ATTA motif within the promoter of the interleukin 6 (IL-6) gene. Using an antisense approach, down-regulation of the expression of GBX2 correlated with decreased expression of IL-6 and an inhibition of tumorigenicity of PC3 human prostate cancer cells. In addition, in vitro growth of the antisense clones was partially restored by exogenous addition of recombinant IL-6 protein to the culture media. These data demonstrated that enhanced GBX2 expression results in a stimulation of malignant growth of prostate cancer cells and that part of this stimulation involves up- regulation in the transcription of the IL-6 gene.",
author = "Gao, {Allen C} and Wei Lou and Isaacs, {John T.}",
year = "2000",
month = "2",
language = "English (US)",
volume = "6",
pages = "493--497",
journal = "Clinical Cancer Research",
issn = "1078-0432",
publisher = "American Association for Cancer Research Inc.",
number = "2",

}

TY - JOUR

T1 - Enhanced GBX2 expression stimulates growth of human prostate cancer cells via transcriptional up-regulation of the interleukin 6 gene

AU - Gao, Allen C

AU - Lou, Wei

AU - Isaacs, John T.

PY - 2000/2

Y1 - 2000/2

N2 - Previous studies demonstrated that the GBX2 homeobox gene is consistently overexpressed in cultured human prostate cancer cell lines. In this study, the human GBX2 cDNA was cloned and a quantitative reverse transcription-PCR method used to demonstrate that GBX2 mRNA expression is enhanced in approximately 70% of human prostate cancer tissues compared with normal human prostate tissues. Purified recombinant GBX2 protein binds specifically to an ATTA motif within the promoter of the interleukin 6 (IL-6) gene. Using an antisense approach, down-regulation of the expression of GBX2 correlated with decreased expression of IL-6 and an inhibition of tumorigenicity of PC3 human prostate cancer cells. In addition, in vitro growth of the antisense clones was partially restored by exogenous addition of recombinant IL-6 protein to the culture media. These data demonstrated that enhanced GBX2 expression results in a stimulation of malignant growth of prostate cancer cells and that part of this stimulation involves up- regulation in the transcription of the IL-6 gene.

AB - Previous studies demonstrated that the GBX2 homeobox gene is consistently overexpressed in cultured human prostate cancer cell lines. In this study, the human GBX2 cDNA was cloned and a quantitative reverse transcription-PCR method used to demonstrate that GBX2 mRNA expression is enhanced in approximately 70% of human prostate cancer tissues compared with normal human prostate tissues. Purified recombinant GBX2 protein binds specifically to an ATTA motif within the promoter of the interleukin 6 (IL-6) gene. Using an antisense approach, down-regulation of the expression of GBX2 correlated with decreased expression of IL-6 and an inhibition of tumorigenicity of PC3 human prostate cancer cells. In addition, in vitro growth of the antisense clones was partially restored by exogenous addition of recombinant IL-6 protein to the culture media. These data demonstrated that enhanced GBX2 expression results in a stimulation of malignant growth of prostate cancer cells and that part of this stimulation involves up- regulation in the transcription of the IL-6 gene.

UR - http://www.scopus.com/inward/record.url?scp=0033963825&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033963825&partnerID=8YFLogxK

M3 - Article

C2 - 10690529

AN - SCOPUS:0033963825

VL - 6

SP - 493

EP - 497

JO - Clinical Cancer Research

JF - Clinical Cancer Research

SN - 1078-0432

IS - 2

ER -