Abstract
Background: Although preferential expression of the Th2 cytokines, IL-4 and IL-5, has been described in atopic asthma, the role of IFN-γ and IL-10 are less clear. Objective: To determine the cytokine pattern of T cell mitogen-induced peripheral blood mononuclear cells obtained from atopic asthmatic (AA) subjects. Methods: Peripheral blood mononuclear cells obtained from AA (n = 24), allergic rhinitis (AR) (n = 9), and normals (NL) (n = 9) were stimulated with phytohemagglutinin (PHA) and the generation of IL-4, IL-5, IFN-γ, IL-10, and GM-CSF was quantified using ELISA. Results: Compared with NL subjects, peripheral blood mononuclear cells from the atopic groups had increased generation of both IL-5 (AA, P = .001 and AR, P = .024) and IFN-γ (AA, P = .037 and AR, P = .048) and decreased generation of IL-10 (AA, P = .038 and AR, P = .036). The absolute levels of cytokines did not differ between the two atopic groups; however, the ratio of IL-5/IL-10 was significantly higher in AA (P < .05), but not in AR when compared with NL subjects. Conclusion: The concomitant increase in the generation of IL-5 and IFN-γ, with a decrease in IL-10 in the atopic groups suggests that in, at least a subset of these patients, there is potential expression of both Th2-and Th1-type cytokines. Furthermore, the increased IL-5 to IL-10 ratio could represent a key feature that distinguishes atopic asthmatic from non-asthmatic atopic subjects.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 115-120 |
| Number of pages | 6 |
| Journal | Annals of Allergy, Asthma and Immunology |
| Volume | 85 |
| Issue number | 2 |
| State | Published - 2000 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology and Allergy
Cite this
Enhanced cytokine generation by peripheral blood mononuclear cells in allergic and asthma subjects. / Kenyon, Nicholas; Kelly, E. A.; Jarjour, N. N.
In: Annals of Allergy, Asthma and Immunology, Vol. 85, No. 2, 2000, p. 115-120.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhanced cytokine generation by peripheral blood mononuclear cells in allergic and asthma subjects
AU - Kenyon, Nicholas
AU - Kelly, E. A.
AU - Jarjour, N. N.
PY - 2000
Y1 - 2000
N2 - Background: Although preferential expression of the Th2 cytokines, IL-4 and IL-5, has been described in atopic asthma, the role of IFN-γ and IL-10 are less clear. Objective: To determine the cytokine pattern of T cell mitogen-induced peripheral blood mononuclear cells obtained from atopic asthmatic (AA) subjects. Methods: Peripheral blood mononuclear cells obtained from AA (n = 24), allergic rhinitis (AR) (n = 9), and normals (NL) (n = 9) were stimulated with phytohemagglutinin (PHA) and the generation of IL-4, IL-5, IFN-γ, IL-10, and GM-CSF was quantified using ELISA. Results: Compared with NL subjects, peripheral blood mononuclear cells from the atopic groups had increased generation of both IL-5 (AA, P = .001 and AR, P = .024) and IFN-γ (AA, P = .037 and AR, P = .048) and decreased generation of IL-10 (AA, P = .038 and AR, P = .036). The absolute levels of cytokines did not differ between the two atopic groups; however, the ratio of IL-5/IL-10 was significantly higher in AA (P < .05), but not in AR when compared with NL subjects. Conclusion: The concomitant increase in the generation of IL-5 and IFN-γ, with a decrease in IL-10 in the atopic groups suggests that in, at least a subset of these patients, there is potential expression of both Th2-and Th1-type cytokines. Furthermore, the increased IL-5 to IL-10 ratio could represent a key feature that distinguishes atopic asthmatic from non-asthmatic atopic subjects.
AB - Background: Although preferential expression of the Th2 cytokines, IL-4 and IL-5, has been described in atopic asthma, the role of IFN-γ and IL-10 are less clear. Objective: To determine the cytokine pattern of T cell mitogen-induced peripheral blood mononuclear cells obtained from atopic asthmatic (AA) subjects. Methods: Peripheral blood mononuclear cells obtained from AA (n = 24), allergic rhinitis (AR) (n = 9), and normals (NL) (n = 9) were stimulated with phytohemagglutinin (PHA) and the generation of IL-4, IL-5, IFN-γ, IL-10, and GM-CSF was quantified using ELISA. Results: Compared with NL subjects, peripheral blood mononuclear cells from the atopic groups had increased generation of both IL-5 (AA, P = .001 and AR, P = .024) and IFN-γ (AA, P = .037 and AR, P = .048) and decreased generation of IL-10 (AA, P = .038 and AR, P = .036). The absolute levels of cytokines did not differ between the two atopic groups; however, the ratio of IL-5/IL-10 was significantly higher in AA (P < .05), but not in AR when compared with NL subjects. Conclusion: The concomitant increase in the generation of IL-5 and IFN-γ, with a decrease in IL-10 in the atopic groups suggests that in, at least a subset of these patients, there is potential expression of both Th2-and Th1-type cytokines. Furthermore, the increased IL-5 to IL-10 ratio could represent a key feature that distinguishes atopic asthmatic from non-asthmatic atopic subjects.
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UR - http://www.scopus.com/inward/citedby.url?scp=0033837952&partnerID=8YFLogxK
M3 - Article
C2 - 10982218
AN - SCOPUS:0033837952
VL - 85
SP - 115
EP - 120
JO - Annals of Allergy, Asthma and Immunology
JF - Annals of Allergy, Asthma and Immunology
SN - 1081-1206
IS - 2
ER -