Enhanced acute responses in an experimental exposure model to biomass smoke inhalation in chronic obstructive pulmonary disease

Jeanine D. Mattson, Brian Haus, Bela Desai, Wayne Ott, Beth Basham, Madhuri Agrawal, Wei Ding, Lynn M. Hildemann, Karin M. Abitorabi, James Canfield, Gordon Mak, Sebnem Guvenc-Tuncturk, Rene De Waal Malefyt, Terrill K. McClanahan, Robert B. Fick, Ware G. Kuschner

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Chronic obstructive pulmonary diseases (COPD) may increase air pollution-related mortality. The relationship of immune mechanisms to mortality caused by fine particulates in healthy and COPD populations is incompletely understood. The objective of this study was to determine whether fine particulates from a single biomass fuel alter stress and inflammation biomarkers in people with COPD. Healthy and COPD subjects were exposed to smoke in a controlled indoor setting. Immune responses were quantified by measuring cell surface marker expression with flow-cytometric analysis and mRNA levels with quantitative reverse transcriptase-polymerase chain reactions in whole blood before and after exposure. Preexposure COPD subjects had more leukocytes, mainly CD14+ monocytes and neutrophils, but fewer CD3+ T cells. Fifty-seven of 186 genes were differentially expressed between healthy and COPD subjects' peripheral blood mononuclear cells (PBMCs). Of these, only nuclear factor (NF)-κ B1, TIMP-1, TIMP-2, and Duffy genes were up-regulated in COPD subjects. At 4 hours post smoke exposure, monocyte levels decreased only in healthy subjects. Fifteen genes, particular to inflammation, immune response, and cell-to-cell signaling, were differentially expressed in COPD subjects, versus 4 genes in healthy subjects. The authors observed significant differences in subjects' PBMCs, which may elucidate the adverse effects of air pollution particulates on people with COPD.

Original languageEnglish (US)
Pages (from-to)631-662
Number of pages32
JournalExperimental Lung Research
Volume34
Issue number10
DOIs
StatePublished - Dec 2008
Externally publishedYes

Fingerprint

Pulmonary diseases
Smoke
Biomass
Chronic Obstructive Pulmonary Disease
Inhalation
Theoretical Models
Genes
Blood
Air Pollution
Air pollution
Monocytes
Blood Cells
Healthy Volunteers
Cell signaling
Inflammation
Tissue Inhibitor of Metalloproteinase-2
Tissue Inhibitor of Metalloproteinase-1
T-cells
Mortality
Polymerase chain reaction

Keywords

  • Chronic obstructive
  • Flow cytometric analysis
  • Forced expiratory flow
  • Forced expiratory volume
  • Forced vital capacity
  • Pulmonary disease
  • Reverse transcriptase-polymerase chain reaction

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Enhanced acute responses in an experimental exposure model to biomass smoke inhalation in chronic obstructive pulmonary disease. / Mattson, Jeanine D.; Haus, Brian; Desai, Bela; Ott, Wayne; Basham, Beth; Agrawal, Madhuri; Ding, Wei; Hildemann, Lynn M.; Abitorabi, Karin M.; Canfield, James; Mak, Gordon; Guvenc-Tuncturk, Sebnem; Malefyt, Rene De Waal; McClanahan, Terrill K.; Fick, Robert B.; Kuschner, Ware G.

In: Experimental Lung Research, Vol. 34, No. 10, 12.2008, p. 631-662.

Research output: Contribution to journalArticle

Mattson, JD, Haus, B, Desai, B, Ott, W, Basham, B, Agrawal, M, Ding, W, Hildemann, LM, Abitorabi, KM, Canfield, J, Mak, G, Guvenc-Tuncturk, S, Malefyt, RDW, McClanahan, TK, Fick, RB & Kuschner, WG 2008, 'Enhanced acute responses in an experimental exposure model to biomass smoke inhalation in chronic obstructive pulmonary disease', Experimental Lung Research, vol. 34, no. 10, pp. 631-662. https://doi.org/10.1080/01902140802322256
Mattson, Jeanine D. ; Haus, Brian ; Desai, Bela ; Ott, Wayne ; Basham, Beth ; Agrawal, Madhuri ; Ding, Wei ; Hildemann, Lynn M. ; Abitorabi, Karin M. ; Canfield, James ; Mak, Gordon ; Guvenc-Tuncturk, Sebnem ; Malefyt, Rene De Waal ; McClanahan, Terrill K. ; Fick, Robert B. ; Kuschner, Ware G. / Enhanced acute responses in an experimental exposure model to biomass smoke inhalation in chronic obstructive pulmonary disease. In: Experimental Lung Research. 2008 ; Vol. 34, No. 10. pp. 631-662.
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