Enhanced acute responses in an experimental exposure model to biomass smoke inhalation in chronic obstructive pulmonary disease

Jeanine D. Mattson, Brian Haus, Bela Desai, Wayne Ott, Beth Basham, Madhuri Agrawal, Wei Ding, Lynn M. Hildemann, Karin M. Abitorabi, James Canfield, Gordon Mak, Sebnem Guvenc-Tuncturk, Rene De Waal Malefyt, Terrill K. McClanahan, Robert B. Fick, Ware G. Kuschner

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Chronic obstructive pulmonary diseases (COPD) may increase air pollution-related mortality. The relationship of immune mechanisms to mortality caused by fine particulates in healthy and COPD populations is incompletely understood. The objective of this study was to determine whether fine particulates from a single biomass fuel alter stress and inflammation biomarkers in people with COPD. Healthy and COPD subjects were exposed to smoke in a controlled indoor setting. Immune responses were quantified by measuring cell surface marker expression with flow-cytometric analysis and mRNA levels with quantitative reverse transcriptase-polymerase chain reactions in whole blood before and after exposure. Preexposure COPD subjects had more leukocytes, mainly CD14+ monocytes and neutrophils, but fewer CD3+ T cells. Fifty-seven of 186 genes were differentially expressed between healthy and COPD subjects' peripheral blood mononuclear cells (PBMCs). Of these, only nuclear factor (NF)-κ B1, TIMP-1, TIMP-2, and Duffy genes were up-regulated in COPD subjects. At 4 hours post smoke exposure, monocyte levels decreased only in healthy subjects. Fifteen genes, particular to inflammation, immune response, and cell-to-cell signaling, were differentially expressed in COPD subjects, versus 4 genes in healthy subjects. The authors observed significant differences in subjects' PBMCs, which may elucidate the adverse effects of air pollution particulates on people with COPD.

Original languageEnglish (US)
Pages (from-to)631-662
Number of pages32
JournalExperimental Lung Research
Issue number10
StatePublished - Dec 2008
Externally publishedYes


  • Chronic obstructive
  • Flow cytometric analysis
  • Forced expiratory flow
  • Forced expiratory volume
  • Forced vital capacity
  • Pulmonary disease
  • Reverse transcriptase-polymerase chain reaction

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Molecular Biology
  • Clinical Biochemistry


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