Engraftment and tumorigenesis of HTLV-1 transformed T cell lines in SCID/bg and NOD/SCID mice

Yingxian Liu, Kiran Dole, James R L Stanley, Virgile Richard, Thomas J. Rosol, Lee Ratner, Michael Dale Lairmore, Gerold Feuer

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Human T cell leukemia/lymphoma virus type-1 (HTLV-1) is recognized as the etiological agent of adult T cell leukemia (ATL). Although HTLV-1 can immortalize human lymphocytes in culture, identification of molecular events leading to tumorigenesis after HTLV-1 infection remain elusive. SCID/bg and NOD/SCID mice have reduced natural killer (NK) cell activity and were inoculated intraperitoneally with HTLV-1 transformed cells to refine and characterize the SCID mouse as a small animal model for investigation of HTLV-1 tumorigenesis. HTLV-1 transformed cell lines originally derived by cocultivation of uninfected peripheral blood mononuclear cells (PBMC) with lethally irradiated leukemic cells from patient samples (SLB-1, MT-2 and HT-1-RV) were lymphomagenic when inoculated into NOD/SCID mice. In contrast, immortalized cell lines generated by transfection PBMC with an infectious molecular clone of HTLV-1 (ACH or ACH.p12) were not tumorigenic. The differing behaviors of HTLV-1 infected cell lines in NOD/SCID mice indicates that viral infection and immortalization of human PBMC for growth in culture is not sufficient for induction of a tumorigenic phenotype. The higher level of engraftment of HTLV-1 transformed cell lines in NOD/SCID mice suggests that this is an effective animal model to investigate molecular determinants of HTLV-1 lymphomagenesis.

Original languageEnglish (US)
Pages (from-to)561-567
Number of pages7
JournalLeukemia Research
Volume26
Issue number6
DOIs
StatePublished - 2002
Externally publishedYes

Keywords

  • ATL
  • HTLV
  • HTLV proviral clone
  • Leukemogenesis
  • SCID mouse
  • Transformation
  • Tumorigenesis

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Fingerprint Dive into the research topics of 'Engraftment and tumorigenesis of HTLV-1 transformed T cell lines in SCID/bg and NOD/SCID mice'. Together they form a unique fingerprint.

  • Cite this

    Liu, Y., Dole, K., Stanley, J. R. L., Richard, V., Rosol, T. J., Ratner, L., Lairmore, M. D., & Feuer, G. (2002). Engraftment and tumorigenesis of HTLV-1 transformed T cell lines in SCID/bg and NOD/SCID mice. Leukemia Research, 26(6), 561-567. https://doi.org/10.1016/S0145-2126(01)00169-2