Engineering the bone-ligament interface using polyethylene glycol diacrylate incorporated with hydroxyapatite

Jennifer Z. Paxton, Kenneth Donnelly, Robert P. Keatch, Keith Baar

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Ligaments and tendons have previously been tissue engineered. However, without the bone attachment, implantation of a tissue-engineered ligament would require it to be sutured to the remnant of the injured native tissue. Due to slow repair and remodeling, this would result in a chronically weak tissue that may never return to preinjury function. In contrast, orthopaedic autograft reconstruction of the ligament often uses a bone-to-bone technique for optimal repair. Since bone-to-bone repairs heal better than other methods, implantation of an artificial ligament should also occur from bone-to-bone. The aim of this study was to investigate the use of a poly(ethylene glycol) diacrylate (PEGDA) hydrogel incorporated with hydroxyapatite (HA) and the cell-adhesion peptide RGD (Arg-Gly-Asp) as a material for creating an in vitro tissue interface to engineer intact ligaments (i.e., bone-ligament-bone). Incorporation of HA into PEG hydrogels reduced the swelling ratio but increased mechanical strength and stiffness of the hydrogels. Further, HA addition increased the capacity for cell growth and interface formation. RGD incorporation increased the swelling ratio but decreased mechanical strength and stiffness of the material. Optimum levels of cell attachment were met using a combination of both HA and RGD, but this material had no better mechanical properties than PEG alone. Although adherence of the hydrogels containing HA was achieved, failure occurs at about 4 days with 5% HA. Increasing the proportion of HA improved interface formation; however, with high levels of HA, the PEG HA composite became brittle. This data suggests that HA, by itself or with other materials, might be well suited for engineering the ligament-bone interface.

Original languageEnglish (US)
Pages (from-to)1201-1209
Number of pages9
JournalTissue Engineering - Part A
Volume15
Issue number6
DOIs
StatePublished - Jun 1 2009
Externally publishedYes

Fingerprint

Ligaments
Durapatite
Hydroxyapatite
Polyethylene glycols
Bone
Bone and Bones
Hydrogels
Tissue
Repair
Strength of materials
Swelling
Stiffness
Hydrogel
Cell adhesion
Tendons
Autografts
Orthopedics
Cell growth
Cell Adhesion
Peptides

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials

Cite this

Engineering the bone-ligament interface using polyethylene glycol diacrylate incorporated with hydroxyapatite. / Paxton, Jennifer Z.; Donnelly, Kenneth; Keatch, Robert P.; Baar, Keith.

In: Tissue Engineering - Part A, Vol. 15, No. 6, 01.06.2009, p. 1201-1209.

Research output: Contribution to journalArticle

Paxton, Jennifer Z. ; Donnelly, Kenneth ; Keatch, Robert P. ; Baar, Keith. / Engineering the bone-ligament interface using polyethylene glycol diacrylate incorporated with hydroxyapatite. In: Tissue Engineering - Part A. 2009 ; Vol. 15, No. 6. pp. 1201-1209.
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