Engineering proteins that bind, move, make and break DNA

Cynthia H. Collins, Yohei Yokobayashi, Daisuke Umeno, Frances H. Arnold

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Recent protein engineering efforts have generated artificial transcription factors that bind new target DNA sequences and enzymes that modify DNA at new target sites. Zinc-finger-based transcription factors are favored targets for design; important technological advances in their construction and numerous biotechnological applications have been reported. Other notable advances include the generation of endonucleases and recombinases with altered specificities, made by innovative combinatorial and evolutionary protein engineering strategies. An unexpectedly high tolerance to mutation in the active sites of DNA polymerases is being exploited to engineer polymerases to incorporate artificial nucleotides or to display other, nonnatural activities.

Original languageEnglish (US)
Pages (from-to)371-378
Number of pages8
JournalCurrent Opinion in Biotechnology
Volume14
Issue number4
DOIs
StatePublished - Aug 2003
Externally publishedYes

Fingerprint

Protein Engineering
DNA Breaks
Transcription factors
DNA
Transcription Factors
Proteins
Recombinases
Endonucleases
DNA sequences
Zinc Fingers
DNA-Directed DNA Polymerase
Nucleotides
Zinc
Catalytic Domain
Enzymes
Engineers
Mutation

ASJC Scopus subject areas

  • Biotechnology

Cite this

Engineering proteins that bind, move, make and break DNA. / Collins, Cynthia H.; Yokobayashi, Yohei; Umeno, Daisuke; Arnold, Frances H.

In: Current Opinion in Biotechnology, Vol. 14, No. 4, 08.2003, p. 371-378.

Research output: Contribution to journalArticle

Collins, CH, Yokobayashi, Y, Umeno, D & Arnold, FH 2003, 'Engineering proteins that bind, move, make and break DNA', Current Opinion in Biotechnology, vol. 14, no. 4, pp. 371-378. https://doi.org/10.1016/S0958-1669(03)00091-0
Collins, Cynthia H. ; Yokobayashi, Yohei ; Umeno, Daisuke ; Arnold, Frances H. / Engineering proteins that bind, move, make and break DNA. In: Current Opinion in Biotechnology. 2003 ; Vol. 14, No. 4. pp. 371-378.
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