Engineering principles for guiding spheroid function in the regeneration of bone, cartilage, and skin

Marissa A. Gionet-Gonzales, Jonathan K Leach

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

There is a critical need for strategies that effectively enhance cell viability and post-implantation performance in order to advance cell-based therapies. Spheroids, which are dense cellular aggregates, overcome many current limitations with transplanting individual cells. Compared to individual cells, the aggregation of cells into spheroids results in increased cell viability, together with enhanced proangiogenic, anti-inflammatory, and tissue-forming potential. Furthermore, the transplantation of cells using engineered materials enables localized delivery to the target site while providing an opportunity to guide cell fate in situ, resulting in improved therapeutic outcomes compared to systemic or localized injection. Despite promising early results achieved by freely injecting spheroids into damaged tissues, growing evidence demonstrates the advantages of entrapping spheroids within a biomaterial prior to implantation. This review will highlight the basic characteristics and qualities of spheroids, describe the underlying principles for how biomaterials influence spheroid behavior, with an emphasis on hydrogels, and provide examples of synergistic approaches using spheroids and biomaterials for tissue engineering applications.

Original languageEnglish (US)
Article number034109
JournalBiomedical Materials (Bristol)
Volume13
Issue number3
DOIs
StatePublished - Mar 21 2018

Fingerprint

Cartilage
Biocompatible Materials
Biomaterials
Skin
Bone
Cells
Tissue
Hydrogels
Tissue engineering
Anti-Inflammatory Agents
Agglomeration

Keywords

  • aggregate
  • biomaterials
  • cell therapy
  • hydrogel
  • spheroid
  • tissue engineering

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Engineering principles for guiding spheroid function in the regeneration of bone, cartilage, and skin. / Gionet-Gonzales, Marissa A.; Leach, Jonathan K.

In: Biomedical Materials (Bristol), Vol. 13, No. 3, 034109, 21.03.2018.

Research output: Contribution to journalArticle

@article{e9ee084637b0416b82aee0c5bf108880,
title = "Engineering principles for guiding spheroid function in the regeneration of bone, cartilage, and skin",
abstract = "There is a critical need for strategies that effectively enhance cell viability and post-implantation performance in order to advance cell-based therapies. Spheroids, which are dense cellular aggregates, overcome many current limitations with transplanting individual cells. Compared to individual cells, the aggregation of cells into spheroids results in increased cell viability, together with enhanced proangiogenic, anti-inflammatory, and tissue-forming potential. Furthermore, the transplantation of cells using engineered materials enables localized delivery to the target site while providing an opportunity to guide cell fate in situ, resulting in improved therapeutic outcomes compared to systemic or localized injection. Despite promising early results achieved by freely injecting spheroids into damaged tissues, growing evidence demonstrates the advantages of entrapping spheroids within a biomaterial prior to implantation. This review will highlight the basic characteristics and qualities of spheroids, describe the underlying principles for how biomaterials influence spheroid behavior, with an emphasis on hydrogels, and provide examples of synergistic approaches using spheroids and biomaterials for tissue engineering applications.",
keywords = "aggregate, biomaterials, cell therapy, hydrogel, spheroid, tissue engineering",
author = "Gionet-Gonzales, {Marissa A.} and Leach, {Jonathan K}",
year = "2018",
month = "3",
day = "21",
doi = "10.1088/1748-605X/aab0b3",
language = "English (US)",
volume = "13",
journal = "Biomedical Materials (Bristol)",
issn = "1748-6041",
publisher = "IOP Publishing Ltd.",
number = "3",

}

TY - JOUR

T1 - Engineering principles for guiding spheroid function in the regeneration of bone, cartilage, and skin

AU - Gionet-Gonzales, Marissa A.

AU - Leach, Jonathan K

PY - 2018/3/21

Y1 - 2018/3/21

N2 - There is a critical need for strategies that effectively enhance cell viability and post-implantation performance in order to advance cell-based therapies. Spheroids, which are dense cellular aggregates, overcome many current limitations with transplanting individual cells. Compared to individual cells, the aggregation of cells into spheroids results in increased cell viability, together with enhanced proangiogenic, anti-inflammatory, and tissue-forming potential. Furthermore, the transplantation of cells using engineered materials enables localized delivery to the target site while providing an opportunity to guide cell fate in situ, resulting in improved therapeutic outcomes compared to systemic or localized injection. Despite promising early results achieved by freely injecting spheroids into damaged tissues, growing evidence demonstrates the advantages of entrapping spheroids within a biomaterial prior to implantation. This review will highlight the basic characteristics and qualities of spheroids, describe the underlying principles for how biomaterials influence spheroid behavior, with an emphasis on hydrogels, and provide examples of synergistic approaches using spheroids and biomaterials for tissue engineering applications.

AB - There is a critical need for strategies that effectively enhance cell viability and post-implantation performance in order to advance cell-based therapies. Spheroids, which are dense cellular aggregates, overcome many current limitations with transplanting individual cells. Compared to individual cells, the aggregation of cells into spheroids results in increased cell viability, together with enhanced proangiogenic, anti-inflammatory, and tissue-forming potential. Furthermore, the transplantation of cells using engineered materials enables localized delivery to the target site while providing an opportunity to guide cell fate in situ, resulting in improved therapeutic outcomes compared to systemic or localized injection. Despite promising early results achieved by freely injecting spheroids into damaged tissues, growing evidence demonstrates the advantages of entrapping spheroids within a biomaterial prior to implantation. This review will highlight the basic characteristics and qualities of spheroids, describe the underlying principles for how biomaterials influence spheroid behavior, with an emphasis on hydrogels, and provide examples of synergistic approaches using spheroids and biomaterials for tissue engineering applications.

KW - aggregate

KW - biomaterials

KW - cell therapy

KW - hydrogel

KW - spheroid

KW - tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=85045733225&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85045733225&partnerID=8YFLogxK

U2 - 10.1088/1748-605X/aab0b3

DO - 10.1088/1748-605X/aab0b3

M3 - Article

C2 - 29460842

AN - SCOPUS:85045733225

VL - 13

JO - Biomedical Materials (Bristol)

JF - Biomedical Materials (Bristol)

SN - 1748-6041

IS - 3

M1 - 034109

ER -