Engineering poliovirus as a vaccine vector for the expression of diverse antigens

Raul Andino, Deborah Silvera, Shelley D. Suggett, Philip L. Achacoso, Chris J Miller, David Baltimore, Mark B. Feinberg

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Abstract

As a step toward developing poliovirus as a vaccine vector, poliovirus recombinants were constructed by fusing exogenous peptides (up to 400 amino acids) and an artificial cleavage site for viral protease 3C(pro) to the amino terminus of the viral polyprotein. Viral replication proceeded normally. An extended polyprotein was produced in infected cells and proteolytically processed into the complete array of viral proteins plus the foreign peptide, which was excluded from mature virions. The recombinants retained exogenous sequences through successive rounds of replication in culture and in vivo. Infection of animals with recombinants elicited a humoral immune response to the foreign peptides.

Original languageEnglish (US)
Pages (from-to)1448-1451
Number of pages4
JournalScience
Volume265
Issue number5177
StatePublished - Sep 2 1994

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Cite this

Andino, R., Silvera, D., Suggett, S. D., Achacoso, P. L., Miller, C. J., Baltimore, D., & Feinberg, M. B. (1994). Engineering poliovirus as a vaccine vector for the expression of diverse antigens. Science, 265(5177), 1448-1451.