Engineering of functional tendon

Sarah Calve, Robert G. Dennis, Paul E. Kosnik, Keith Baar, Karl Grosh, Ellen M. Arruda

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

Surgical tendon repair is limited by the availability of viable tissue for transplantation. Because of its relatively avascular nature, tendon is a prime candidate for engineered tissue replacement. To address this problem, cells isolated from rat Achilles tendon were grown to confluence in culture and allowed to self-assemble into a cylinder between two anchor points. The resulting scaffold-free tissue was composed of aligned, small-diameter collagen fibrils, a large number of cells, and an excess of noncollagenous extracellular matrix; all characteristics of embryonic tendon. The stress-strain response of the constructs also resembles the nonlinear behavior of immature tendons, and the ultimate tensile strength is approximately equal to that of embryonic chick tendon, roughly 2 MPa. These physical and mechanical properties indicate that these constructs are the first viable tendons engineered in vitro, without the aid of artificial scaffolding.

Original languageEnglish (US)
Pages (from-to)755-761
Number of pages7
JournalTissue Engineering
Volume10
Issue number5-6
DOIs
StatePublished - May 2004
Externally publishedYes

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Tendons
Tissue
Tissue Scaffolds
Tissue Transplantation
Achilles Tendon
Tensile Strength
Extracellular Matrix
Anchors
Collagen
Scaffolds
Cell Count
Rats
Repair
Tensile strength
Physical properties
Availability
Mechanical properties

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology

Cite this

Calve, S., Dennis, R. G., Kosnik, P. E., Baar, K., Grosh, K., & Arruda, E. M. (2004). Engineering of functional tendon. Tissue Engineering, 10(5-6), 755-761. https://doi.org/10.1089/1076327041348464

Engineering of functional tendon. / Calve, Sarah; Dennis, Robert G.; Kosnik, Paul E.; Baar, Keith; Grosh, Karl; Arruda, Ellen M.

In: Tissue Engineering, Vol. 10, No. 5-6, 05.2004, p. 755-761.

Research output: Contribution to journalArticle

Calve, S, Dennis, RG, Kosnik, PE, Baar, K, Grosh, K & Arruda, EM 2004, 'Engineering of functional tendon', Tissue Engineering, vol. 10, no. 5-6, pp. 755-761. https://doi.org/10.1089/1076327041348464
Calve S, Dennis RG, Kosnik PE, Baar K, Grosh K, Arruda EM. Engineering of functional tendon. Tissue Engineering. 2004 May;10(5-6):755-761. https://doi.org/10.1089/1076327041348464
Calve, Sarah ; Dennis, Robert G. ; Kosnik, Paul E. ; Baar, Keith ; Grosh, Karl ; Arruda, Ellen M. / Engineering of functional tendon. In: Tissue Engineering. 2004 ; Vol. 10, No. 5-6. pp. 755-761.
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