Engineering mesenchymal stem cells to improve their exosome efficacy and yield for cell-free therapy

Jennifer Phan, Priyadarsini Kumar, Dake Hao, Kewa Gao, Diana L Farmer, Aijun Wang

Research output: Contribution to journalReview article

38 Scopus citations

Abstract

Through traditional medicine, there were diseases and disorders that previously remained untreated or were simply thought to be incurable. Since the discovery of mesenchymal stem cells (MSCs), there has been a flurry of research to develop MSC-based therapy for diseases and disorders. It is now well-known that MSCs do not typically engraft after transplantation and exhibit their therapeutic effect via a paracrine mechanism. In addition to secretory proteins, MSCs also produce extracellular vesicles (EVs), membrane-bound nanovesicles containing proteins, DNA and RNA. The secreted vesicles then interact with target cells and deliver their contents, imparting their ultimate therapeutic effect. Unlike the widely studied cancer cells, the yield of MSC-exosomes is a limiting factor for large-scale production for cell-free therapies. Here we summarise potential approaches to increase the yield of such vesicles while maintaining or enhancing their efficacy by engineering the extracellular environment and intracellular components of MSCs.

Original languageEnglish (US)
Article number1522236
JournalJournal of Extracellular Vesicles
Volume7
Issue number1
DOIs
StatePublished - Jan 1 2018

Keywords

  • extracellular vesicles
  • Mesenchymal stem cells

ASJC Scopus subject areas

  • Histology
  • Cell Biology

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