Engineering host resistance against parasitic weeds with RNA interference

John I. Yoder, Pradeepa Gunathilake, Biao Wu, Natalya Tomilova, Alexey Tomilov

Research output: Contribution to journalReview article

36 Citations (Scopus)

Abstract

Host genetic resistance is a key component of integrated pest management. The present authors and others are investigating the use of RNA interference (RNAi) as a genetic tool for engineering host resistance against parasitic weeds. The general approach is to transform a host plant with a plasmid encoding a double stranded hairpin RNA (hpRNA) targeted against one or more vital parasite genes. When the hpRNAs are specifically designed against parasite gene sequences, the hpRNA should have no phenotypic effect on the host. They will, however, have a dramatic effect on the parasites that have taken up the parasite-specific RNAi from the host via the haustorium. The current status of using RNAi technology for controlling parasitic weeds is reviewed. A key component to success with RNAi technology is identifying the best parasite genes to silence. Some of the criteria for RNAi targets are discussed, the existing status of parasitic plant sequence databases is described and internet access points to the parasite genome data are highlighted. Sequence information obtained from different parasite species can be used to clone the homologous gene from a particular pest or can be directly transformed into crop plants.

Original languageEnglish (US)
Pages (from-to)460-466
Number of pages7
JournalPest Management Science
Volume65
Issue number5
DOIs
StatePublished - May 1 2009

Fingerprint

parasitic plants
RNA interference
engineering
parasites
RNA
genes
genetic resistance
integrated pest management
plasmids
host plants
pests
clones
nucleotide sequences
genome
crops

Keywords

  • Engineered resistance
  • Genetic resistance
  • Parasitic plant
  • Triphysaria

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Insect Science

Cite this

Engineering host resistance against parasitic weeds with RNA interference. / Yoder, John I.; Gunathilake, Pradeepa; Wu, Biao; Tomilova, Natalya; Tomilov, Alexey.

In: Pest Management Science, Vol. 65, No. 5, 01.05.2009, p. 460-466.

Research output: Contribution to journalReview article

Yoder, John I. ; Gunathilake, Pradeepa ; Wu, Biao ; Tomilova, Natalya ; Tomilov, Alexey. / Engineering host resistance against parasitic weeds with RNA interference. In: Pest Management Science. 2009 ; Vol. 65, No. 5. pp. 460-466.
@article{b525a210d20246ce86a615d8f6f2aa0e,
title = "Engineering host resistance against parasitic weeds with RNA interference",
abstract = "Host genetic resistance is a key component of integrated pest management. The present authors and others are investigating the use of RNA interference (RNAi) as a genetic tool for engineering host resistance against parasitic weeds. The general approach is to transform a host plant with a plasmid encoding a double stranded hairpin RNA (hpRNA) targeted against one or more vital parasite genes. When the hpRNAs are specifically designed against parasite gene sequences, the hpRNA should have no phenotypic effect on the host. They will, however, have a dramatic effect on the parasites that have taken up the parasite-specific RNAi from the host via the haustorium. The current status of using RNAi technology for controlling parasitic weeds is reviewed. A key component to success with RNAi technology is identifying the best parasite genes to silence. Some of the criteria for RNAi targets are discussed, the existing status of parasitic plant sequence databases is described and internet access points to the parasite genome data are highlighted. Sequence information obtained from different parasite species can be used to clone the homologous gene from a particular pest or can be directly transformed into crop plants.",
keywords = "Engineered resistance, Genetic resistance, Parasitic plant, Triphysaria",
author = "Yoder, {John I.} and Pradeepa Gunathilake and Biao Wu and Natalya Tomilova and Alexey Tomilov",
year = "2009",
month = "5",
day = "1",
doi = "10.1002/ps.1696",
language = "English (US)",
volume = "65",
pages = "460--466",
journal = "Pest Management Science",
issn = "1526-498X",
publisher = "John Wiley and Sons Ltd",
number = "5",

}

TY - JOUR

T1 - Engineering host resistance against parasitic weeds with RNA interference

AU - Yoder, John I.

AU - Gunathilake, Pradeepa

AU - Wu, Biao

AU - Tomilova, Natalya

AU - Tomilov, Alexey

PY - 2009/5/1

Y1 - 2009/5/1

N2 - Host genetic resistance is a key component of integrated pest management. The present authors and others are investigating the use of RNA interference (RNAi) as a genetic tool for engineering host resistance against parasitic weeds. The general approach is to transform a host plant with a plasmid encoding a double stranded hairpin RNA (hpRNA) targeted against one or more vital parasite genes. When the hpRNAs are specifically designed against parasite gene sequences, the hpRNA should have no phenotypic effect on the host. They will, however, have a dramatic effect on the parasites that have taken up the parasite-specific RNAi from the host via the haustorium. The current status of using RNAi technology for controlling parasitic weeds is reviewed. A key component to success with RNAi technology is identifying the best parasite genes to silence. Some of the criteria for RNAi targets are discussed, the existing status of parasitic plant sequence databases is described and internet access points to the parasite genome data are highlighted. Sequence information obtained from different parasite species can be used to clone the homologous gene from a particular pest or can be directly transformed into crop plants.

AB - Host genetic resistance is a key component of integrated pest management. The present authors and others are investigating the use of RNA interference (RNAi) as a genetic tool for engineering host resistance against parasitic weeds. The general approach is to transform a host plant with a plasmid encoding a double stranded hairpin RNA (hpRNA) targeted against one or more vital parasite genes. When the hpRNAs are specifically designed against parasite gene sequences, the hpRNA should have no phenotypic effect on the host. They will, however, have a dramatic effect on the parasites that have taken up the parasite-specific RNAi from the host via the haustorium. The current status of using RNAi technology for controlling parasitic weeds is reviewed. A key component to success with RNAi technology is identifying the best parasite genes to silence. Some of the criteria for RNAi targets are discussed, the existing status of parasitic plant sequence databases is described and internet access points to the parasite genome data are highlighted. Sequence information obtained from different parasite species can be used to clone the homologous gene from a particular pest or can be directly transformed into crop plants.

KW - Engineered resistance

KW - Genetic resistance

KW - Parasitic plant

KW - Triphysaria

UR - http://www.scopus.com/inward/record.url?scp=65649116328&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65649116328&partnerID=8YFLogxK

U2 - 10.1002/ps.1696

DO - 10.1002/ps.1696

M3 - Review article

VL - 65

SP - 460

EP - 466

JO - Pest Management Science

JF - Pest Management Science

SN - 1526-498X

IS - 5

ER -