Engineering and application of genetically encoded calcium indicators

Jasper Akerboom, Lin Tian, Jonathan S. Marvin, Loren L. Looger

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Genetically encoded fluorescent biosensors are useful tools for tracking target analytes in cells, tissues and living organisms. These probes are often chimeric proteins consisting of a recognition element (e.g., a ligand-binding protein) and a reporter element (one or more fluorescent proteins). The analyte-induced conformational change in the recognition element leads to an observable change in fluorescence in the reporter element. Expression of biosensors is noninvasive and can be targeted to specific tissues and cell types using specific promoter and enhancer sequences, and to subcellular compartments with signal peptides and retention tags. Recent improvements in both indicator engineering and microscopy methods enable chronic in vivo measurements. Here, we describe methods used in the design, testing, optimization and application of genetically encoded biosensors, with a particular focus on the widely utilized calcium indicator GCaMP.

Original languageEnglish (US)
Title of host publicationNeuromethods
Pages125-147
Number of pages23
Volume72
DOIs
StatePublished - 2012

Publication series

NameNeuromethods
Volume72
ISSN (Print)08932336
ISSN (Electronic)19406045

Fingerprint

Biosensing Techniques
Biosensors
Calcium
Tissue
Protein Sorting Signals
Target tracking
Microscopy
Carrier Proteins
Microscopic examination
Proteins
Fluorescence
Ligands
Testing

Keywords

  • Calcium imaging
  • GCaMP
  • GCaMP3
  • GECI
  • Neural activity imaging
  • Protein engineering

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Neuroscience(all)
  • Psychiatry and Mental health

Cite this

Akerboom, J., Tian, L., Marvin, J. S., & Looger, L. L. (2012). Engineering and application of genetically encoded calcium indicators. In Neuromethods (Vol. 72, pp. 125-147). (Neuromethods; Vol. 72). https://doi.org/10.1007/978-1-62703-014-4-8

Engineering and application of genetically encoded calcium indicators. / Akerboom, Jasper; Tian, Lin; Marvin, Jonathan S.; Looger, Loren L.

Neuromethods. Vol. 72 2012. p. 125-147 (Neuromethods; Vol. 72).

Research output: Chapter in Book/Report/Conference proceedingChapter

Akerboom, J, Tian, L, Marvin, JS & Looger, LL 2012, Engineering and application of genetically encoded calcium indicators. in Neuromethods. vol. 72, Neuromethods, vol. 72, pp. 125-147. https://doi.org/10.1007/978-1-62703-014-4-8
Akerboom J, Tian L, Marvin JS, Looger LL. Engineering and application of genetically encoded calcium indicators. In Neuromethods. Vol. 72. 2012. p. 125-147. (Neuromethods). https://doi.org/10.1007/978-1-62703-014-4-8
Akerboom, Jasper ; Tian, Lin ; Marvin, Jonathan S. ; Looger, Loren L. / Engineering and application of genetically encoded calcium indicators. Neuromethods. Vol. 72 2012. pp. 125-147 (Neuromethods).
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