Engineering anastomosis between biological perfused vessel networks and endothelial cell-lined microfluidic channels

X. Wang, D. Phan, Steven George, C. C.W. Hughes, A. P. Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports, for the first time, a microfluidic system that can reproduce the vascular anastomosis between a perfused vessel network in a 3D tissue chamber and an endothelial cell (EC)-lined microfluidic channel. By using a decoupling design of the microfluidic device, the physiological microenvironment including both the interstitial flow for vasculogenesis and shear stress for EC lining can be well controlled. To facilitate the anastomosis process, the basal-to-apical transendothelial flow as well as the vascular endothelial growth factor (VEGF) gradient are involved in promoting the sprouting angiogenesis from the EC monolayer into the fibrin gel.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages748-750
Number of pages3
ISBN (Electronic)9780979806476
StatePublished - Jan 1 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

Keywords

  • Anastomosis
  • Angiogenesis
  • Endothelial cell lining
  • Vasculogenesis

ASJC Scopus subject areas

  • Control and Systems Engineering

Fingerprint Dive into the research topics of 'Engineering anastomosis between biological perfused vessel networks and endothelial cell-lined microfluidic channels'. Together they form a unique fingerprint.

  • Cite this

    Wang, X., Phan, D., George, S., Hughes, C. C. W., & Lee, A. P. (2014). Engineering anastomosis between biological perfused vessel networks and endothelial cell-lined microfluidic channels. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 748-750). Chemical and Biological Microsystems Society.