Engagement of the adhesion receptor CD22 triggers a potent stimulatory signal for B cells and blocking CD22/CD22L interactions impairs T-cell proliferation

Joseph Tuscano, Pablo Engel, Thomas F. Tedder, John H. Kehrl

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

The B-lymphocyte-restricted adhesion protein CD22 mediates sialic acid- dependent cell-cell interactions. Engagement of CD22 on B lymphocytes with a CD22 monoclonal antibody (MoAb) HB22.7 that blocks the binding of CD22 to its ligand(s) directly stimulated B-cell proliferation. In addition, the HB22.7 MoAb costimulated B-cell proliferation with either anti-IgM, interleukin-2 (IL-2), IL-4, or CD40 and triggered predominantly B-cell IgG secretion with IL-2. Even more striking levels of B-cell proliferation occurred with HB22.7 MoAb under culture conditions that enhanced B-B-cell interactions. In contrast, a nonblocking CD22 MoAb (CD22.5) poorly costimulated in similar experiments. The functional differences between the two antibodies likely result from differing abilities to trigger downstream signaling events as significant differences in CD22 tyrosine phosphorylation and the recruitment of the tyrosine kinase p53/56lyn and the tyrosine phosphatase SH-PTP1C were found. Besides their role in B-cell stimulation, CD22/CD22L interactions may also assist in regulating T-cell proliferation because inhibition of CD22- CD22L engagement with the HB22.7 MoAb impaired T-cell proliferation in a costimulatory assay. Thus, CD22/CD22L interactions result in stimulatory signals for both B and T lymphocytes.

Original languageEnglish (US)
Pages (from-to)4723-4730
Number of pages8
JournalBlood
Volume87
Issue number11
StatePublished - Jun 1 1996
Externally publishedYes

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T-cells
Cell proliferation
B-Lymphocytes
Monoclonal Antibodies
Cells
Cell Proliferation
T-Lymphocytes
Lymphocytes
Interleukin-2
Tyrosine
Phosphorylation
N-Acetylneuraminic Acid
Cell Communication
Cell culture
Phosphoric Monoester Hydrolases
Interleukin-4
Protein-Tyrosine Kinases
Assays
Adhesion
Immunoglobulin G

ASJC Scopus subject areas

  • Hematology

Cite this

Engagement of the adhesion receptor CD22 triggers a potent stimulatory signal for B cells and blocking CD22/CD22L interactions impairs T-cell proliferation. / Tuscano, Joseph; Engel, Pablo; Tedder, Thomas F.; Kehrl, John H.

In: Blood, Vol. 87, No. 11, 01.06.1996, p. 4723-4730.

Research output: Contribution to journalArticle

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