Energy-sensitive cryogenic detectors for high-mass biomolecule mass spectrometry

Matthias Frank, Simon E. Labov, Garrett Westmacott, W. Henry Benner

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Energy-sensitive calorimetric detectors that operate at low temperatures ("cryogenic detectors") have recently been applied for the first time as ion detectors in time-of-flight mass spectrometry. Compared to conventional, ionization-based detectors, which rely on secondary electron formation or the charge created in a semiconductor, cryogenic detectors measure low-energy solid state excitations created by a particle impact. This energy sensitivity of cryogenic detectors results in several potential advantages for TOP-MS. Cryogenic detectors are expected to have near 100% efficiency even for very large, slow-moving molecules, in contrast to microchannel plates whose efficiency drops considerably at large mass. Thus, cryogenic detectors could contribute to extending the mass range accessible by TOF-MS and help improving detection limits. In addition, the energy resolution provided by cryogenic detectors can be used for charge discrimination and studies of ion fragmentation, ion-detector interaction, and internal energies of large molecular ions. Cryogenic detectors could therefore prove to be a valuable diagnostic tool in TOF-MS. Here, we give a general introduction to the cryogenic detector types most applicable to TOF-MS including those types already used in several TOF-MS experiments. We review and compare the results of these experiments, discuss practical aspects of operating cryogenic detectors in TOF-MS systems, and describe potential near future improvements of cryogenic detectors for applications in mass spectrometry.

Original languageEnglish (US)
Pages (from-to)155-186
Number of pages32
JournalMass Spectrometry Reviews
Volume18
Issue number3-4
StatePublished - May 1 1999
Externally publishedYes

Fingerprint

Biomolecules
Cryogenics
cryogenics
Mass spectrometry
Mass Spectrometry
mass spectroscopy
Ions
Detectors
detectors
Semiconductors
energy
Limit of Detection
Electrons
Temperature
ions
microchannel plates
Microchannels
internal energy
molecular ions
Ionization

Keywords

  • Calorimetric detectors
  • Cryogenic detectors
  • Highmass detectors
  • Time-of-flight mass spectrometry

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Condensed Matter Physics
  • Spectroscopy

Cite this

Frank, M., Labov, S. E., Westmacott, G., & Benner, W. H. (1999). Energy-sensitive cryogenic detectors for high-mass biomolecule mass spectrometry. Mass Spectrometry Reviews, 18(3-4), 155-186.

Energy-sensitive cryogenic detectors for high-mass biomolecule mass spectrometry. / Frank, Matthias; Labov, Simon E.; Westmacott, Garrett; Benner, W. Henry.

In: Mass Spectrometry Reviews, Vol. 18, No. 3-4, 01.05.1999, p. 155-186.

Research output: Contribution to journalArticle

Frank, M, Labov, SE, Westmacott, G & Benner, WH 1999, 'Energy-sensitive cryogenic detectors for high-mass biomolecule mass spectrometry', Mass Spectrometry Reviews, vol. 18, no. 3-4, pp. 155-186.
Frank, Matthias ; Labov, Simon E. ; Westmacott, Garrett ; Benner, W. Henry. / Energy-sensitive cryogenic detectors for high-mass biomolecule mass spectrometry. In: Mass Spectrometry Reviews. 1999 ; Vol. 18, No. 3-4. pp. 155-186.
@article{59a6a2e5981f4f7099c3509b21a7e4fc,
title = "Energy-sensitive cryogenic detectors for high-mass biomolecule mass spectrometry",
abstract = "Energy-sensitive calorimetric detectors that operate at low temperatures ({"}cryogenic detectors{"}) have recently been applied for the first time as ion detectors in time-of-flight mass spectrometry. Compared to conventional, ionization-based detectors, which rely on secondary electron formation or the charge created in a semiconductor, cryogenic detectors measure low-energy solid state excitations created by a particle impact. This energy sensitivity of cryogenic detectors results in several potential advantages for TOP-MS. Cryogenic detectors are expected to have near 100{\%} efficiency even for very large, slow-moving molecules, in contrast to microchannel plates whose efficiency drops considerably at large mass. Thus, cryogenic detectors could contribute to extending the mass range accessible by TOF-MS and help improving detection limits. In addition, the energy resolution provided by cryogenic detectors can be used for charge discrimination and studies of ion fragmentation, ion-detector interaction, and internal energies of large molecular ions. Cryogenic detectors could therefore prove to be a valuable diagnostic tool in TOF-MS. Here, we give a general introduction to the cryogenic detector types most applicable to TOF-MS including those types already used in several TOF-MS experiments. We review and compare the results of these experiments, discuss practical aspects of operating cryogenic detectors in TOF-MS systems, and describe potential near future improvements of cryogenic detectors for applications in mass spectrometry.",
keywords = "Calorimetric detectors, Cryogenic detectors, Highmass detectors, Time-of-flight mass spectrometry",
author = "Matthias Frank and Labov, {Simon E.} and Garrett Westmacott and Benner, {W. Henry}",
year = "1999",
month = "5",
day = "1",
language = "English (US)",
volume = "18",
pages = "155--186",
journal = "Mass Spectrometry Reviews",
issn = "0277-7037",
publisher = "John Wiley and Sons Inc.",
number = "3-4",

}

TY - JOUR

T1 - Energy-sensitive cryogenic detectors for high-mass biomolecule mass spectrometry

AU - Frank, Matthias

AU - Labov, Simon E.

AU - Westmacott, Garrett

AU - Benner, W. Henry

PY - 1999/5/1

Y1 - 1999/5/1

N2 - Energy-sensitive calorimetric detectors that operate at low temperatures ("cryogenic detectors") have recently been applied for the first time as ion detectors in time-of-flight mass spectrometry. Compared to conventional, ionization-based detectors, which rely on secondary electron formation or the charge created in a semiconductor, cryogenic detectors measure low-energy solid state excitations created by a particle impact. This energy sensitivity of cryogenic detectors results in several potential advantages for TOP-MS. Cryogenic detectors are expected to have near 100% efficiency even for very large, slow-moving molecules, in contrast to microchannel plates whose efficiency drops considerably at large mass. Thus, cryogenic detectors could contribute to extending the mass range accessible by TOF-MS and help improving detection limits. In addition, the energy resolution provided by cryogenic detectors can be used for charge discrimination and studies of ion fragmentation, ion-detector interaction, and internal energies of large molecular ions. Cryogenic detectors could therefore prove to be a valuable diagnostic tool in TOF-MS. Here, we give a general introduction to the cryogenic detector types most applicable to TOF-MS including those types already used in several TOF-MS experiments. We review and compare the results of these experiments, discuss practical aspects of operating cryogenic detectors in TOF-MS systems, and describe potential near future improvements of cryogenic detectors for applications in mass spectrometry.

AB - Energy-sensitive calorimetric detectors that operate at low temperatures ("cryogenic detectors") have recently been applied for the first time as ion detectors in time-of-flight mass spectrometry. Compared to conventional, ionization-based detectors, which rely on secondary electron formation or the charge created in a semiconductor, cryogenic detectors measure low-energy solid state excitations created by a particle impact. This energy sensitivity of cryogenic detectors results in several potential advantages for TOP-MS. Cryogenic detectors are expected to have near 100% efficiency even for very large, slow-moving molecules, in contrast to microchannel plates whose efficiency drops considerably at large mass. Thus, cryogenic detectors could contribute to extending the mass range accessible by TOF-MS and help improving detection limits. In addition, the energy resolution provided by cryogenic detectors can be used for charge discrimination and studies of ion fragmentation, ion-detector interaction, and internal energies of large molecular ions. Cryogenic detectors could therefore prove to be a valuable diagnostic tool in TOF-MS. Here, we give a general introduction to the cryogenic detector types most applicable to TOF-MS including those types already used in several TOF-MS experiments. We review and compare the results of these experiments, discuss practical aspects of operating cryogenic detectors in TOF-MS systems, and describe potential near future improvements of cryogenic detectors for applications in mass spectrometry.

KW - Calorimetric detectors

KW - Cryogenic detectors

KW - Highmass detectors

KW - Time-of-flight mass spectrometry

UR - http://www.scopus.com/inward/record.url?scp=0033126156&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033126156&partnerID=8YFLogxK

M3 - Article

C2 - 10568040

AN - SCOPUS:0033126156

VL - 18

SP - 155

EP - 186

JO - Mass Spectrometry Reviews

JF - Mass Spectrometry Reviews

SN - 0277-7037

IS - 3-4

ER -