Energy-dependence of nitrate reductase induction in Candida utilis

Prabhakara V Choudary, G. Ramananda Rao

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The requirement of a suitable energy source during the induced synthesis of nitrate reductase in Candida utilis was investigated. The levels of nitrate reductase induced were shown to be energy-dependent, and to vary in response to the type of carbon source provided. Glycerol, fructose, ethanol, glucose, and sucrose served as efficient energy sources. Growth rate of the yeast and the induced level of nitrate reductase were dependent on the ratio of carbon to nitrogen in the induction medium, and ratio of 2 being optimal. Induction of nitrate reductase was inhibited by uncouplers, 2,4-dinitrophenol (DNP), dicumarol and carbonyl cyanide p-trifluoromethoxy phenyl hydrazone (CCCP), and by cyanide and azide, indicating an absolute energy-dependency. The facilitation of induction of a high level of nitrate reductase by exogenously added ATP as sole source of energy confirmed the obligate requirement of ATP for the synthesis of nitrate reductase in Candidautilis.

Original languageEnglish (US)
Pages (from-to)1293-1299
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume108
Issue number3
DOIs
StatePublished - Oct 15 1982
Externally publishedYes

Fingerprint

Nitrate Reductase
Candida
Carbon
Adenosine Triphosphate
Carbonyl Cyanide m-Chlorophenyl Hydrazone
Dicumarol
2,4-Dinitrophenol
Hydrazones
Azides
Cyanides
Fructose
Yeast
Glycerol
Sucrose
Ethanol
Nitrogen
Yeasts
Glucose
Growth

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Energy-dependence of nitrate reductase induction in Candida utilis. / Choudary, Prabhakara V; Rao, G. Ramananda.

In: Biochemical and Biophysical Research Communications, Vol. 108, No. 3, 15.10.1982, p. 1293-1299.

Research output: Contribution to journalArticle

Choudary, Prabhakara V ; Rao, G. Ramananda. / Energy-dependence of nitrate reductase induction in Candida utilis. In: Biochemical and Biophysical Research Communications. 1982 ; Vol. 108, No. 3. pp. 1293-1299.
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