Endurance training restores spatially distinct cardiac mitochondrial function and myocardial contractility in ovariectomized rats

Elis Aguiar Morra, Paula Lopes Rodrigues, Itamar Couto Guedes de Jesus, Patrícia Ribeiro Do Val Lima, Renata Andrade Ávila, Tadeu Ériton Caliman Zanardo, Breno Valentim Nogueira, Donald M Bers, Silvia Guatimosim, Ivanita Stefanon, Rogério Faustino Ribeiro Júnior

Research output: Contribution to journalArticle

Abstract

We previously demonstrated that the loss of female hormones induces cardiac and mitochondrial dysfunction in the female heart. Here, we show the impact of endurance training for twelve weeks, a nonpharmacological therapy against cardiovascular disease caused by ovariectomy and its contribution to cardiac contractility, mitochondrial quality control, bioenergetics and oxidative damage. We found that ovariectomy induced cardiac hypertrophy and dysfunction by decreasing SERCA2 and increasing phospholamban protein expression. Endurance training restored myocardial contractility, SERCA2 levels, increased calcium transient in ovariectomized rats but did not change phospholamban protein expression or cardiac hypertrophy. Additionally, ovariectomy decreased the amount of intermyofibrillar mitochondria and induced mitochondrial fragmentation that were accompanied by decreased levels of mitofusin 1, PGC-1α, NRF-1, total AMPK-α and mitochondrial Tfam. Endurance training prevented all these features except for mitofusin 1. Ovariectomy reduced O2 consumption, elevated O2 .- release and increased Ca2+-induced mitochondrial permeability transition pore opening in both mitochondrial subpopulations. Ovariectomy also increased NOX-4 protein expression in the heart, reduced mitochondrial Mn-SOD, catalase protein expression and increased protein carbonylation in both mitochondrial subpopulations, which were prevented by endurance training. Taken together, our findings show that endurance training prevented cardiac contractile dysfunction and mitochondrial quality control in ovariectomized rats.

LanguageEnglish (US)
Pages174-188
Number of pages15
JournalFree Radical Biology and Medicine
Volume130
DOIs
StatePublished - Jan 1 2019

Fingerprint

Ovariectomy
Rats
Durability
Cardiomegaly
Proteins
Quality Control
Quality control
Protein Carbonylation
Carbonylation
AMP-Activated Protein Kinases
Mitochondria
Catalase
Energy Metabolism
Superoxide Dismutase
Cardiovascular Diseases
Hormones
Calcium
phospholamban

Keywords

  • Bioenergetics
  • Cardiac dysfunction
  • Endurance training
  • Female hormone deprivation
  • Intermyofibrillar and subsarcolemmal mitochondria
  • Mitochondrial quality control

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

Morra, E. A., Rodrigues, P. L., Jesus, I. C. G. D., Do Val Lima, P. R., Ávila, R. A., Zanardo, T. É. C., ... Ribeiro Júnior, R. F. (2019). Endurance training restores spatially distinct cardiac mitochondrial function and myocardial contractility in ovariectomized rats. Free Radical Biology and Medicine, 130, 174-188. https://doi.org/10.1016/j.freeradbiomed.2018.10.406

Endurance training restores spatially distinct cardiac mitochondrial function and myocardial contractility in ovariectomized rats. / Morra, Elis Aguiar; Rodrigues, Paula Lopes; Jesus, Itamar Couto Guedes de; Do Val Lima, Patrícia Ribeiro; Ávila, Renata Andrade; Zanardo, Tadeu Ériton Caliman; Nogueira, Breno Valentim; Bers, Donald M; Guatimosim, Silvia; Stefanon, Ivanita; Ribeiro Júnior, Rogério Faustino.

In: Free Radical Biology and Medicine, Vol. 130, 01.01.2019, p. 174-188.

Research output: Contribution to journalArticle

Morra, EA, Rodrigues, PL, Jesus, ICGD, Do Val Lima, PR, Ávila, RA, Zanardo, TÉC, Nogueira, BV, Bers, DM, Guatimosim, S, Stefanon, I & Ribeiro Júnior, RF 2019, 'Endurance training restores spatially distinct cardiac mitochondrial function and myocardial contractility in ovariectomized rats' Free Radical Biology and Medicine, vol. 130, pp. 174-188. https://doi.org/10.1016/j.freeradbiomed.2018.10.406
Morra, Elis Aguiar ; Rodrigues, Paula Lopes ; Jesus, Itamar Couto Guedes de ; Do Val Lima, Patrícia Ribeiro ; Ávila, Renata Andrade ; Zanardo, Tadeu Ériton Caliman ; Nogueira, Breno Valentim ; Bers, Donald M ; Guatimosim, Silvia ; Stefanon, Ivanita ; Ribeiro Júnior, Rogério Faustino. / Endurance training restores spatially distinct cardiac mitochondrial function and myocardial contractility in ovariectomized rats. In: Free Radical Biology and Medicine. 2019 ; Vol. 130. pp. 174-188.
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AU - Zanardo, Tadeu Ériton Caliman

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