Endothelial inflammation correlates with subject triglycerides and waist size after a high-fat meal

Ying I. Wang, John Schulze, Nadine Raymond, Tyler Tomita, Kayan Tam, Scott I. Simon, Anthony G. Passerini

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

A rise in postprandial serum triglycerides (PP-sTG) can potentiate inflammatory responses in vascular endothelial cells (ECs) and thus serves as an independent risk factor for predicting increased cardiovascular morbidity. We examined postprandial triglyceride-rich lipoproteins (PP-TGRLs) in subjects ranging from normal to hypertriglyceridemic for their capacity to alter EC acute inflammatory responses. Cultured human aortic ECs (HAECs) were conditioned with PP-TGRLs isolated from human serum at the peak after a moderately high-fat meal. VLDL particle size increased postprandially and varied directly with the subject's PP-sTG level and waist circumference. PP-TGRL particles bound to HAECs and were internalized via LDL receptor-mediated endocytosis. PP-TGRL alone did not induce an inflammatory response over the range of individuals studied. However, combined with low-dose TNF-α stimulation (0.3 ng/ml), it elicited a net 10-15% increase above cytokine alone in the membrane expression of VCAM-1, ICAM-1, and E-selectin, which was not observed with fasting TGRLs. In contrast to upregulation of ICAM-1 and E-selectin, VCAM-1 transcription and expression varied in direct proportion with individual PP-sTG and waist circumference. The extent of monocyte arrest on inflamed HAECs under shear stress also correlated closely with VCAM-1 expression induced by conditioning with PP-TGRL and TNF-α stimulation. This ex vivo approach provides a quantitative means to assess an individual's inflammatory potential, revealing a greater propensity for endothelial inflammation in hypertriglyceridemic individuals with abdominal obesity.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume300
Issue number3
DOIs
StatePublished - Mar 2011

Fingerprint

Meals
Triglycerides
Fats
Inflammation
Lipoproteins
Vascular Cell Adhesion Molecule-1
E-Selectin
Endothelial Cells
Waist Circumference
Intercellular Adhesion Molecule-1
Serum
Abdominal Obesity
LDL Receptors
Endocytosis
Particle Size
Monocytes
Fasting
Up-Regulation
Cytokines
Morbidity

Keywords

  • Atherosclerosis
  • Dyslipidemia
  • Endothelial cells
  • Triglyceride-rich lipoprotein
  • Tumor necrosis factor-a
  • Vascular cell adhesion molecule-1

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine

Cite this

Endothelial inflammation correlates with subject triglycerides and waist size after a high-fat meal. / Wang, Ying I.; Schulze, John; Raymond, Nadine; Tomita, Tyler; Tam, Kayan; Simon, Scott I.; Passerini, Anthony G.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 300, No. 3, 03.2011.

Research output: Contribution to journalArticle

Wang, Ying I. ; Schulze, John ; Raymond, Nadine ; Tomita, Tyler ; Tam, Kayan ; Simon, Scott I. ; Passerini, Anthony G. / Endothelial inflammation correlates with subject triglycerides and waist size after a high-fat meal. In: American Journal of Physiology - Heart and Circulatory Physiology. 2011 ; Vol. 300, No. 3.
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