Endothelial glycocalyx

Permeability barrier and mechanosensor

F. E. Curry, R. H. Adamson

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

Endothelial cells are covered with a polysaccha-ride rich layer more than 400 nm thick, mechanical properties of which limit access of circulating plasma components to endothelial cell membranes. The barrier properties of this endothelial surface layer are deduced from the rate of tracer penetration into the layer and the mechanics of red and white cell movement through capillary microvessels. This review compares the mechanosensor and permeability properties of an inner layer (100-150 nm, close to the endothelial membrane) characterized as a quasi-periodic structure which accounts for key aspects of transvascular exchange and vascular permeability with those of the whole endothelial surface layers. We conclude that many of the barrier properties of the whole surface layer are not representative of the primary fiber matrix forming the molecular filter determining transvascular exchange. The differences between the properties of the whole layer and the inner glycocalyx structures likely reflect dynamic aspects of the endothelial surface layer including tracer binding to specific components, synthesis and degradation of key components, activation of signaling pathways in the endothelial cells when components of the surface layer are lost or degraded, and the spatial distribution of adhesion proteins in microdomains of the endothelial cell membrane.

Original languageEnglish (US)
Pages (from-to)828-839
Number of pages12
JournalAnnals of Biomedical Engineering
Volume40
Issue number4
DOIs
StatePublished - Apr 2012

Fingerprint

Endothelial cells
Cell membranes
Periodic structures
Spatial distribution
Mechanics
Adhesion
Chemical activation
Cells
Proteins
Membranes
Plasmas
Degradation
Mechanical properties
Fibers

Keywords

  • Edema
  • Endothe-lium
  • Fiber matrix
  • Fluid balance
  • Inflammation
  • Shear stress
  • Vascular

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Endothelial glycocalyx : Permeability barrier and mechanosensor. / Curry, F. E.; Adamson, R. H.

In: Annals of Biomedical Engineering, Vol. 40, No. 4, 04.2012, p. 828-839.

Research output: Contribution to journalArticle

Curry, F. E. ; Adamson, R. H. / Endothelial glycocalyx : Permeability barrier and mechanosensor. In: Annals of Biomedical Engineering. 2012 ; Vol. 40, No. 4. pp. 828-839.
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