Endomicroscopy imaging of epithelial structures using tissue autofluorescence

Bevin Lin, Shiro Urayama, Ramez M G Saroufeem, Dennis L Matthews, Stavros G. Demos

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We explore autofluorescence endomicroscopy as a potential tool for real-time visualization of epithelial tissue microstructure and organization in a clinical setting. The design parameters are explored using two experimental systems - an Olympus Medical Systems Corp. stand-alone clinical prototype probe, and a custom built bench-top rigid fiber conduit prototype. Both systems entail ultraviolet excitation at 266 nm and/or 325 nm using compact laser sources. Preliminary results using ex vivo animal and human tissue specimens suggest that this technology can be translated toward in vivo application to address the need for real-time histology.

Original languageEnglish (US)
Article number046014
JournalJournal of Biomedical Optics
Volume16
Issue number4
DOIs
StatePublished - Apr 2011

Fingerprint

prototypes
Tissue
Imaging techniques
Histology
histology
seats
animals
Animals
Visualization
microstructure
Microstructure
fibers
Fibers
Lasers
probes
excitation
lasers

Keywords

  • Endomicroscopy
  • Real-time histology
  • Ultraviolet autofluorescence

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Endomicroscopy imaging of epithelial structures using tissue autofluorescence. / Lin, Bevin; Urayama, Shiro; Saroufeem, Ramez M G; Matthews, Dennis L; Demos, Stavros G.

In: Journal of Biomedical Optics, Vol. 16, No. 4, 046014, 04.2011.

Research output: Contribution to journalArticle

@article{e7e4642ec8d340028306d42ac5b8e15d,
title = "Endomicroscopy imaging of epithelial structures using tissue autofluorescence",
abstract = "We explore autofluorescence endomicroscopy as a potential tool for real-time visualization of epithelial tissue microstructure and organization in a clinical setting. The design parameters are explored using two experimental systems - an Olympus Medical Systems Corp. stand-alone clinical prototype probe, and a custom built bench-top rigid fiber conduit prototype. Both systems entail ultraviolet excitation at 266 nm and/or 325 nm using compact laser sources. Preliminary results using ex vivo animal and human tissue specimens suggest that this technology can be translated toward in vivo application to address the need for real-time histology.",
keywords = "Endomicroscopy, Real-time histology, Ultraviolet autofluorescence",
author = "Bevin Lin and Shiro Urayama and Saroufeem, {Ramez M G} and Matthews, {Dennis L} and Demos, {Stavros G.}",
year = "2011",
month = "4",
doi = "10.1117/1.3565216",
language = "English (US)",
volume = "16",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",
publisher = "SPIE",
number = "4",

}

TY - JOUR

T1 - Endomicroscopy imaging of epithelial structures using tissue autofluorescence

AU - Lin, Bevin

AU - Urayama, Shiro

AU - Saroufeem, Ramez M G

AU - Matthews, Dennis L

AU - Demos, Stavros G.

PY - 2011/4

Y1 - 2011/4

N2 - We explore autofluorescence endomicroscopy as a potential tool for real-time visualization of epithelial tissue microstructure and organization in a clinical setting. The design parameters are explored using two experimental systems - an Olympus Medical Systems Corp. stand-alone clinical prototype probe, and a custom built bench-top rigid fiber conduit prototype. Both systems entail ultraviolet excitation at 266 nm and/or 325 nm using compact laser sources. Preliminary results using ex vivo animal and human tissue specimens suggest that this technology can be translated toward in vivo application to address the need for real-time histology.

AB - We explore autofluorescence endomicroscopy as a potential tool for real-time visualization of epithelial tissue microstructure and organization in a clinical setting. The design parameters are explored using two experimental systems - an Olympus Medical Systems Corp. stand-alone clinical prototype probe, and a custom built bench-top rigid fiber conduit prototype. Both systems entail ultraviolet excitation at 266 nm and/or 325 nm using compact laser sources. Preliminary results using ex vivo animal and human tissue specimens suggest that this technology can be translated toward in vivo application to address the need for real-time histology.

KW - Endomicroscopy

KW - Real-time histology

KW - Ultraviolet autofluorescence

UR - http://www.scopus.com/inward/record.url?scp=80455163277&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80455163277&partnerID=8YFLogxK

U2 - 10.1117/1.3565216

DO - 10.1117/1.3565216

M3 - Article

C2 - 21529083

AN - SCOPUS:80455163277

VL - 16

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

SN - 1083-3668

IS - 4

M1 - 046014

ER -