Abstract
Postpartum endometritis compromises milk production and fertility in high-producing dairy cows. Infection of the endometrium induces an inflammatory response with secretion of cytokines that lead to polymorphonuclear cells (PMN) influx and bacterial clearance. Considering that only a portion of cows with endometritis is eligible for clinical diagnosis, there is an increasing effort for developing reliable tools and protocols for diagnosis of subclinical endometritis. Recent reports have indicated that primiparous cows are at greater risk of uterine infection and primiparous cows with subclinical endometritis produce less milk compared to healthy cows. In the present study, gene expression profiles were compared for selected cytokine and hormone endometrial transcripts in the postpartum of primiparous Holstein cows with clinical and subclinical endometritis. Cows were classified as healthy (no signs of clinical endometritis), cows with subclinical endometritis (PMN. <. 5% in the cytological sample) and cows with clinical endometritis (PMN. >. 5%). Although, cows with clinical endometritis had greater (. P<. 0.05) relative amounts of mRNA for the IL1A, IL6, IL17A, TNFα, PGES and PGHS2 genes compared to healthy cows; no significant differences were detected between clinical and subclinical endometritis groups. Spearman correlation coefficients were positive between relative amounts of gene expression as indicated by amount of these transcripts and PMN percentages and ranged from 0.74 to 0.93 (. P<. 0.05). Relative amounts of cytokine mRNA suggest similar inflammatory response in the endometrium of cows with subclinical and clinical endometritis. Moreover, differential relative amounts of hormone transcripts suggest dysregulation of the luteolytic mechanism and PG synthases but not ERα in cows with endometritis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 34-39 |
| Number of pages | 6 |
| Journal | Animal Reproduction Science |
| Volume | 156 |
| DOIs | |
| State | Published - May 1 2015 |
| Externally published | Yes |
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Keywords
- Dairy cow
- Endometritis
- Interleukin
- Prostaglandin
ASJC Scopus subject areas
- Animal Science and Zoology
- Food Animals
- Endocrinology
Cite this
Endometrial expression of selected transcripts in postpartum of primiparous Holstein cows with clinical and subclinical endometritis. / Johnson, Howard; Torres, Cristian G.; Carvallo chaigneau, Francisco R; Duchens, Mario; Peralta, Oscar A.
In: Animal Reproduction Science, Vol. 156, 01.05.2015, p. 34-39.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Endometrial expression of selected transcripts in postpartum of primiparous Holstein cows with clinical and subclinical endometritis
AU - Johnson, Howard
AU - Torres, Cristian G.
AU - Carvallo chaigneau, Francisco R
AU - Duchens, Mario
AU - Peralta, Oscar A.
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Postpartum endometritis compromises milk production and fertility in high-producing dairy cows. Infection of the endometrium induces an inflammatory response with secretion of cytokines that lead to polymorphonuclear cells (PMN) influx and bacterial clearance. Considering that only a portion of cows with endometritis is eligible for clinical diagnosis, there is an increasing effort for developing reliable tools and protocols for diagnosis of subclinical endometritis. Recent reports have indicated that primiparous cows are at greater risk of uterine infection and primiparous cows with subclinical endometritis produce less milk compared to healthy cows. In the present study, gene expression profiles were compared for selected cytokine and hormone endometrial transcripts in the postpartum of primiparous Holstein cows with clinical and subclinical endometritis. Cows were classified as healthy (no signs of clinical endometritis), cows with subclinical endometritis (PMN. <. 5% in the cytological sample) and cows with clinical endometritis (PMN. >. 5%). Although, cows with clinical endometritis had greater (. P<. 0.05) relative amounts of mRNA for the IL1A, IL6, IL17A, TNFα, PGES and PGHS2 genes compared to healthy cows; no significant differences were detected between clinical and subclinical endometritis groups. Spearman correlation coefficients were positive between relative amounts of gene expression as indicated by amount of these transcripts and PMN percentages and ranged from 0.74 to 0.93 (. P<. 0.05). Relative amounts of cytokine mRNA suggest similar inflammatory response in the endometrium of cows with subclinical and clinical endometritis. Moreover, differential relative amounts of hormone transcripts suggest dysregulation of the luteolytic mechanism and PG synthases but not ERα in cows with endometritis.
AB - Postpartum endometritis compromises milk production and fertility in high-producing dairy cows. Infection of the endometrium induces an inflammatory response with secretion of cytokines that lead to polymorphonuclear cells (PMN) influx and bacterial clearance. Considering that only a portion of cows with endometritis is eligible for clinical diagnosis, there is an increasing effort for developing reliable tools and protocols for diagnosis of subclinical endometritis. Recent reports have indicated that primiparous cows are at greater risk of uterine infection and primiparous cows with subclinical endometritis produce less milk compared to healthy cows. In the present study, gene expression profiles were compared for selected cytokine and hormone endometrial transcripts in the postpartum of primiparous Holstein cows with clinical and subclinical endometritis. Cows were classified as healthy (no signs of clinical endometritis), cows with subclinical endometritis (PMN. <. 5% in the cytological sample) and cows with clinical endometritis (PMN. >. 5%). Although, cows with clinical endometritis had greater (. P<. 0.05) relative amounts of mRNA for the IL1A, IL6, IL17A, TNFα, PGES and PGHS2 genes compared to healthy cows; no significant differences were detected between clinical and subclinical endometritis groups. Spearman correlation coefficients were positive between relative amounts of gene expression as indicated by amount of these transcripts and PMN percentages and ranged from 0.74 to 0.93 (. P<. 0.05). Relative amounts of cytokine mRNA suggest similar inflammatory response in the endometrium of cows with subclinical and clinical endometritis. Moreover, differential relative amounts of hormone transcripts suggest dysregulation of the luteolytic mechanism and PG synthases but not ERα in cows with endometritis.
KW - Dairy cow
KW - Endometritis
KW - Interleukin
KW - Prostaglandin
UR - http://www.scopus.com/inward/record.url?scp=84928208205&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84928208205&partnerID=8YFLogxK
U2 - 10.1016/j.anireprosci.2015.02.007
DO - 10.1016/j.anireprosci.2015.02.007
M3 - Article
C2 - 25746855
AN - SCOPUS:84928208205
VL - 156
SP - 34
EP - 39
JO - Animal Reproduction Science
JF - Animal Reproduction Science
SN - 0378-4320
ER -