Endometrial expression of selected transcripts in postpartum of primiparous Holstein cows with clinical and subclinical endometritis

Howard Johnson, Cristian G. Torres, Francisco R Carvallo chaigneau, Mario Duchens, Oscar A. Peralta

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Postpartum endometritis compromises milk production and fertility in high-producing dairy cows. Infection of the endometrium induces an inflammatory response with secretion of cytokines that lead to polymorphonuclear cells (PMN) influx and bacterial clearance. Considering that only a portion of cows with endometritis is eligible for clinical diagnosis, there is an increasing effort for developing reliable tools and protocols for diagnosis of subclinical endometritis. Recent reports have indicated that primiparous cows are at greater risk of uterine infection and primiparous cows with subclinical endometritis produce less milk compared to healthy cows. In the present study, gene expression profiles were compared for selected cytokine and hormone endometrial transcripts in the postpartum of primiparous Holstein cows with clinical and subclinical endometritis. Cows were classified as healthy (no signs of clinical endometritis), cows with subclinical endometritis (PMN. <. 5% in the cytological sample) and cows with clinical endometritis (PMN. >. 5%). Although, cows with clinical endometritis had greater (. P<. 0.05) relative amounts of mRNA for the IL1A, IL6, IL17A, TNFα, PGES and PGHS2 genes compared to healthy cows; no significant differences were detected between clinical and subclinical endometritis groups. Spearman correlation coefficients were positive between relative amounts of gene expression as indicated by amount of these transcripts and PMN percentages and ranged from 0.74 to 0.93 (. P<. 0.05). Relative amounts of cytokine mRNA suggest similar inflammatory response in the endometrium of cows with subclinical and clinical endometritis. Moreover, differential relative amounts of hormone transcripts suggest dysregulation of the luteolytic mechanism and PG synthases but not ERα in cows with endometritis.

Original languageEnglish (US)
Pages (from-to)34-39
Number of pages6
JournalAnimal Reproduction Science
Volume156
DOIs
StatePublished - May 1 2015
Externally publishedYes

Fingerprint

Endometritis
endometritis
Postpartum Period
Holstein
cows
cytokines
endometrium
Cytokines
Endometrium
Milk
inflammation
hormones
Hormones
gene expression
Messenger RNA
cells
Infection
Transcriptome
infection
Fertility

Keywords

  • Dairy cow
  • Endometritis
  • Interleukin
  • Prostaglandin

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Animals
  • Endocrinology

Cite this

Endometrial expression of selected transcripts in postpartum of primiparous Holstein cows with clinical and subclinical endometritis. / Johnson, Howard; Torres, Cristian G.; Carvallo chaigneau, Francisco R; Duchens, Mario; Peralta, Oscar A.

In: Animal Reproduction Science, Vol. 156, 01.05.2015, p. 34-39.

Research output: Contribution to journalArticle

@article{b1065ab684bf4933a24727e90d49e213,
title = "Endometrial expression of selected transcripts in postpartum of primiparous Holstein cows with clinical and subclinical endometritis",
abstract = "Postpartum endometritis compromises milk production and fertility in high-producing dairy cows. Infection of the endometrium induces an inflammatory response with secretion of cytokines that lead to polymorphonuclear cells (PMN) influx and bacterial clearance. Considering that only a portion of cows with endometritis is eligible for clinical diagnosis, there is an increasing effort for developing reliable tools and protocols for diagnosis of subclinical endometritis. Recent reports have indicated that primiparous cows are at greater risk of uterine infection and primiparous cows with subclinical endometritis produce less milk compared to healthy cows. In the present study, gene expression profiles were compared for selected cytokine and hormone endometrial transcripts in the postpartum of primiparous Holstein cows with clinical and subclinical endometritis. Cows were classified as healthy (no signs of clinical endometritis), cows with subclinical endometritis (PMN. <. 5{\%} in the cytological sample) and cows with clinical endometritis (PMN. >. 5{\%}). Although, cows with clinical endometritis had greater (. P<. 0.05) relative amounts of mRNA for the IL1A, IL6, IL17A, TNFα, PGES and PGHS2 genes compared to healthy cows; no significant differences were detected between clinical and subclinical endometritis groups. Spearman correlation coefficients were positive between relative amounts of gene expression as indicated by amount of these transcripts and PMN percentages and ranged from 0.74 to 0.93 (. P<. 0.05). Relative amounts of cytokine mRNA suggest similar inflammatory response in the endometrium of cows with subclinical and clinical endometritis. Moreover, differential relative amounts of hormone transcripts suggest dysregulation of the luteolytic mechanism and PG synthases but not ERα in cows with endometritis.",
keywords = "Dairy cow, Endometritis, Interleukin, Prostaglandin",
author = "Howard Johnson and Torres, {Cristian G.} and {Carvallo chaigneau}, {Francisco R} and Mario Duchens and Peralta, {Oscar A.}",
year = "2015",
month = "5",
day = "1",
doi = "10.1016/j.anireprosci.2015.02.007",
language = "English (US)",
volume = "156",
pages = "34--39",
journal = "Animal Reproduction Science",
issn = "0378-4320",
publisher = "Elsevier",

}

TY - JOUR

T1 - Endometrial expression of selected transcripts in postpartum of primiparous Holstein cows with clinical and subclinical endometritis

AU - Johnson, Howard

AU - Torres, Cristian G.

AU - Carvallo chaigneau, Francisco R

AU - Duchens, Mario

AU - Peralta, Oscar A.

PY - 2015/5/1

Y1 - 2015/5/1

N2 - Postpartum endometritis compromises milk production and fertility in high-producing dairy cows. Infection of the endometrium induces an inflammatory response with secretion of cytokines that lead to polymorphonuclear cells (PMN) influx and bacterial clearance. Considering that only a portion of cows with endometritis is eligible for clinical diagnosis, there is an increasing effort for developing reliable tools and protocols for diagnosis of subclinical endometritis. Recent reports have indicated that primiparous cows are at greater risk of uterine infection and primiparous cows with subclinical endometritis produce less milk compared to healthy cows. In the present study, gene expression profiles were compared for selected cytokine and hormone endometrial transcripts in the postpartum of primiparous Holstein cows with clinical and subclinical endometritis. Cows were classified as healthy (no signs of clinical endometritis), cows with subclinical endometritis (PMN. <. 5% in the cytological sample) and cows with clinical endometritis (PMN. >. 5%). Although, cows with clinical endometritis had greater (. P<. 0.05) relative amounts of mRNA for the IL1A, IL6, IL17A, TNFα, PGES and PGHS2 genes compared to healthy cows; no significant differences were detected between clinical and subclinical endometritis groups. Spearman correlation coefficients were positive between relative amounts of gene expression as indicated by amount of these transcripts and PMN percentages and ranged from 0.74 to 0.93 (. P<. 0.05). Relative amounts of cytokine mRNA suggest similar inflammatory response in the endometrium of cows with subclinical and clinical endometritis. Moreover, differential relative amounts of hormone transcripts suggest dysregulation of the luteolytic mechanism and PG synthases but not ERα in cows with endometritis.

AB - Postpartum endometritis compromises milk production and fertility in high-producing dairy cows. Infection of the endometrium induces an inflammatory response with secretion of cytokines that lead to polymorphonuclear cells (PMN) influx and bacterial clearance. Considering that only a portion of cows with endometritis is eligible for clinical diagnosis, there is an increasing effort for developing reliable tools and protocols for diagnosis of subclinical endometritis. Recent reports have indicated that primiparous cows are at greater risk of uterine infection and primiparous cows with subclinical endometritis produce less milk compared to healthy cows. In the present study, gene expression profiles were compared for selected cytokine and hormone endometrial transcripts in the postpartum of primiparous Holstein cows with clinical and subclinical endometritis. Cows were classified as healthy (no signs of clinical endometritis), cows with subclinical endometritis (PMN. <. 5% in the cytological sample) and cows with clinical endometritis (PMN. >. 5%). Although, cows with clinical endometritis had greater (. P<. 0.05) relative amounts of mRNA for the IL1A, IL6, IL17A, TNFα, PGES and PGHS2 genes compared to healthy cows; no significant differences were detected between clinical and subclinical endometritis groups. Spearman correlation coefficients were positive between relative amounts of gene expression as indicated by amount of these transcripts and PMN percentages and ranged from 0.74 to 0.93 (. P<. 0.05). Relative amounts of cytokine mRNA suggest similar inflammatory response in the endometrium of cows with subclinical and clinical endometritis. Moreover, differential relative amounts of hormone transcripts suggest dysregulation of the luteolytic mechanism and PG synthases but not ERα in cows with endometritis.

KW - Dairy cow

KW - Endometritis

KW - Interleukin

KW - Prostaglandin

UR - http://www.scopus.com/inward/record.url?scp=84928208205&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84928208205&partnerID=8YFLogxK

U2 - 10.1016/j.anireprosci.2015.02.007

DO - 10.1016/j.anireprosci.2015.02.007

M3 - Article

C2 - 25746855

AN - SCOPUS:84928208205

VL - 156

SP - 34

EP - 39

JO - Animal Reproduction Science

JF - Animal Reproduction Science

SN - 0378-4320

ER -