Endogenous galectin-3 is localized in membrane lipid rafts and regulates migration of dendritic cells

Daniel K. Hsu, Alexander I. Chernyavsky, Huan Yuan Chen, Lan Yu, Sergei A. Grando, Fu-Tong Liu

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

This study reveals a function of endogenous galectin-3, an animal lectin recognizing β-galactosides, in regulating dendritic cell motility both in vitro and in vivo, which to our knowledge is unreported. First, galectin-3-deficient (gal3-/-) bone marrow-derived dendritic cells exhibited defective chemotaxis compared to gal3+/+ cells. Second, cutaneous dendritic cells in gal3-/- mice displayed reduced migration to draining lymph nodes upon hapten stimulation compared to gal3+/+ mice. Moreover, gal3-/- mice were impaired in the development of contact hypersensitivity relative to gal3+/+ mice in response to a hapten, a process in which dendritic cell trafficking to lymph nodes is critical. In addition, defective signaling was detected in gal3-/- cells upon chemokine receptor activation. By immunofluorescence microscopy, we observed that galectin-3 is localized in membrane ruffles and lamellipodia in stimulated dendritic cells and macrophages. Furthermore, galectin-3 was enriched in lipid raft domains under these conditions. Finally, we determined that ruffles on gal3-/- cells contained structures with lower complexity compared to gal3+/+ cells. In view of the participation of membrane ruffles in signal transduction and cell motility, we conclude that galectin-3 regulates cell migration by functioning at these structures.

Original languageEnglish (US)
Pages (from-to)573-583
Number of pages11
JournalJournal of Investigative Dermatology
Volume129
Issue number3
DOIs
StatePublished - Mar 2009

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Galectin 3
Membrane Lipids
Dendritic Cells
Cell Movement
Haptens
Lymph Nodes
Membranes
Galactosides
Signal transduction
Pseudopodia
Langerhans Cells
Macrophages
Chemokine Receptors
Contact Dermatitis
Chemotaxis
Fluorescence Microscopy
Lectins
Signal Transduction
Microscopic examination
Bone

ASJC Scopus subject areas

  • Dermatology
  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Endogenous galectin-3 is localized in membrane lipid rafts and regulates migration of dendritic cells. / Hsu, Daniel K.; Chernyavsky, Alexander I.; Chen, Huan Yuan; Yu, Lan; Grando, Sergei A.; Liu, Fu-Tong.

In: Journal of Investigative Dermatology, Vol. 129, No. 3, 03.2009, p. 573-583.

Research output: Contribution to journalArticle

Hsu, Daniel K. ; Chernyavsky, Alexander I. ; Chen, Huan Yuan ; Yu, Lan ; Grando, Sergei A. ; Liu, Fu-Tong. / Endogenous galectin-3 is localized in membrane lipid rafts and regulates migration of dendritic cells. In: Journal of Investigative Dermatology. 2009 ; Vol. 129, No. 3. pp. 573-583.
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