Endogenous CD8+ T cell expansion during regression of monoclonal EBV- associated posttransplant lymphoproliferative disorder

Vijay P. Khatri, Robert A. Baiocchi, Ruoqi Peng, Adam R. Oberkircher, Jean M. Dolce, Pamela M. Ward, Geoffrey P. Herzig, Michael A. Caligiuri

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

There are experimental data which suggest that the primary immune effector cell responsible for maintaining immune surveillance against the outgrowth of EBV-transformed B cells in humans is the CTL, but in vivo proof of this is lacking. In this study we perform a series of cellular and molecular assays to characterize an autologous, endogenous immune response against a transplantation-associated, monoclonal, EBV+ posttransplant lymphoproliferative disorder (PTLD). Following allogeneic bone marrow transplantation, a patient developed a monoclonal PTLD of donor B cell origin. With a decrease in immune suppression, we document the emergence of endogenous, donor-derived CD3+CD8+ CTLs, followed by regression of the PTLD. The TCR Vβ repertoire went from a polyclonal pattern prior to the development of PTLD to a restricted TCR Vβ pattern during the outgrowth and regression of PTLD. Donor-derived CD3+CD8+ T lymphocytes displayed MHC class I-restricted cytolytic activity against the autologous EBV+ B cells ex vivo without additional in vitro sensitization. The striking temporal relationship between the endogenous expansion of a TCR Vβ-restricted, CD3+CD8+ population of MHC class I-restricted CTL, and the regression of an autologous monoclonal PTLD, provides direct evidence in humans that endogenous CD3+CD8+ CTLs can be responsible for effective immune surveillance against malignant transformation of EBV+ B cells.

Original languageEnglish (US)
Pages (from-to)500-506
Number of pages7
JournalJournal of Immunology
Volume163
Issue number1
StatePublished - Jul 1 1999
Externally publishedYes

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Lymphoproliferative Disorders
Human Herpesvirus 4
T-Lymphocytes
B-Lymphocytes
Tissue Donors
Homologous Transplantation
Bone Marrow Transplantation
Transplantation
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Khatri, V. P., Baiocchi, R. A., Peng, R., Oberkircher, A. R., Dolce, J. M., Ward, P. M., ... Caligiuri, M. A. (1999). Endogenous CD8+ T cell expansion during regression of monoclonal EBV- associated posttransplant lymphoproliferative disorder. Journal of Immunology, 163(1), 500-506.

Endogenous CD8+ T cell expansion during regression of monoclonal EBV- associated posttransplant lymphoproliferative disorder. / Khatri, Vijay P.; Baiocchi, Robert A.; Peng, Ruoqi; Oberkircher, Adam R.; Dolce, Jean M.; Ward, Pamela M.; Herzig, Geoffrey P.; Caligiuri, Michael A.

In: Journal of Immunology, Vol. 163, No. 1, 01.07.1999, p. 500-506.

Research output: Contribution to journalArticle

Khatri, VP, Baiocchi, RA, Peng, R, Oberkircher, AR, Dolce, JM, Ward, PM, Herzig, GP & Caligiuri, MA 1999, 'Endogenous CD8+ T cell expansion during regression of monoclonal EBV- associated posttransplant lymphoproliferative disorder', Journal of Immunology, vol. 163, no. 1, pp. 500-506.
Khatri VP, Baiocchi RA, Peng R, Oberkircher AR, Dolce JM, Ward PM et al. Endogenous CD8+ T cell expansion during regression of monoclonal EBV- associated posttransplant lymphoproliferative disorder. Journal of Immunology. 1999 Jul 1;163(1):500-506.
Khatri, Vijay P. ; Baiocchi, Robert A. ; Peng, Ruoqi ; Oberkircher, Adam R. ; Dolce, Jean M. ; Ward, Pamela M. ; Herzig, Geoffrey P. ; Caligiuri, Michael A. / Endogenous CD8+ T cell expansion during regression of monoclonal EBV- associated posttransplant lymphoproliferative disorder. In: Journal of Immunology. 1999 ; Vol. 163, No. 1. pp. 500-506.
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