Abstract
Whether all cells ascribed to the same type are indeed identical and the related question, "How are signals integrated at tissue and cell levels?" are fundamental issues in biological research and are responsible, in part, for the acceleration of activity in single-cell research. Also contributing are the major advances in single cell methodology. The capability to interrogate, manipulate with spatiotemporal specificity, and acquire data on multiple activities in the same cell can be powerful for understanding normal function and also dysfunction and drug targeting. Reviewed here are examples of how the emergent field of single-cell analysis and its tools are being used to understand the dynamics of the multiple components in regulatory networks for genes, intracellular signaling molecules, and secretion. For more in-depth consideration, the pancreatic β-cell and the pituitary gonadotrope are reviewed as parallel examples for application of single-cell analysis in the examination of one component of regulated secretion.
| Original language | English (US) |
|---|---|
| Title of host publication | Cellular Endocrinology in Health and Disease |
| Publisher | Elsevier Inc. |
| Pages | 325-340 |
| Number of pages | 16 |
| ISBN (Print) | 9780124081345 |
| DOIs | |
| State | Published - May 2014 |
Fingerprint
Keywords
- β-cell
- Epac2
- Exocytosis
- Fluorescence microscopy
- G protein-coupled receptors (GPCRs)
- Gonadotrope
- Optogenetics
- Rap1
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Endocrinology of the Single Cell. Tools and Insights. / Turgeon, Judith L; Waring, Dennis W.
Cellular Endocrinology in Health and Disease. Elsevier Inc., 2014. p. 325-340.Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Endocrinology of the Single Cell. Tools and Insights.
AU - Turgeon, Judith L
AU - Waring, Dennis W.
PY - 2014/5
Y1 - 2014/5
N2 - Whether all cells ascribed to the same type are indeed identical and the related question, "How are signals integrated at tissue and cell levels?" are fundamental issues in biological research and are responsible, in part, for the acceleration of activity in single-cell research. Also contributing are the major advances in single cell methodology. The capability to interrogate, manipulate with spatiotemporal specificity, and acquire data on multiple activities in the same cell can be powerful for understanding normal function and also dysfunction and drug targeting. Reviewed here are examples of how the emergent field of single-cell analysis and its tools are being used to understand the dynamics of the multiple components in regulatory networks for genes, intracellular signaling molecules, and secretion. For more in-depth consideration, the pancreatic β-cell and the pituitary gonadotrope are reviewed as parallel examples for application of single-cell analysis in the examination of one component of regulated secretion.
AB - Whether all cells ascribed to the same type are indeed identical and the related question, "How are signals integrated at tissue and cell levels?" are fundamental issues in biological research and are responsible, in part, for the acceleration of activity in single-cell research. Also contributing are the major advances in single cell methodology. The capability to interrogate, manipulate with spatiotemporal specificity, and acquire data on multiple activities in the same cell can be powerful for understanding normal function and also dysfunction and drug targeting. Reviewed here are examples of how the emergent field of single-cell analysis and its tools are being used to understand the dynamics of the multiple components in regulatory networks for genes, intracellular signaling molecules, and secretion. For more in-depth consideration, the pancreatic β-cell and the pituitary gonadotrope are reviewed as parallel examples for application of single-cell analysis in the examination of one component of regulated secretion.
KW - β-cell
KW - Epac2
KW - Exocytosis
KW - Fluorescence microscopy
KW - G protein-coupled receptors (GPCRs)
KW - Gonadotrope
KW - Optogenetics
KW - Rap1
UR - http://www.scopus.com/inward/record.url?scp=84902402056&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84902402056&partnerID=8YFLogxK
U2 - 10.1016/B978-0-12-408134-5.00020-2
DO - 10.1016/B978-0-12-408134-5.00020-2
M3 - Chapter
SN - 9780124081345
SP - 325
EP - 340
BT - Cellular Endocrinology in Health and Disease
PB - Elsevier Inc.
ER -