Endocrine antecedents of polycystic ovary syndrome in fetal and infant prenatally androgenized female rhesus monkeys

David H. Abbott, Deborah K. Barnett, Jon E. Levine, Vasantha Padmanabhan, Daniel A. Dumesic, Steve Jacoris, Alice F Tarantal

Research output: Contribution to journalArticlepeer-review

79 Scopus citations


Experimentally induced fetal androgen excess induces polycystic ovary syndrome-like traits in adult female rhesus monkeys (Macaca mulatta). Developmental changes leading to this endocrinopathy are not known. We therefore studied 15 time-mated, gravid female rhesus monkeys with known female fetuses. Nine dams received daily s.c. injections of 15 mg of testosterone propionate (TP), and six received injections of oil vehicle (control) from 40 through 80 days of gestation (term, 165 days; range, ±10 days). All fetuses were delivered by cesarean section using established methods at term. Ultrasound-guided fetal blood sample collection and peripheral venous sample collection of dams and subsequent infants enabled determination of circulating levels of steroid hormones, LH and FSH. The TP injections elevated serum testosterone and androstenedione levels in the dams and prenatally androgenized (PA) fetuses. After cessation of TP injections, testosterone levels returned to values within the reference range for animals in these age groups, whereas serum androstenedione levels in PA infants were elevated. The TP injections did not increase estrogen levels in the dams or the PA fetuses or infants, yet conjugated estrogen levels were elevated in the TP-injected dams. Serum levels of LH and FSH were elevated in late-gestation PA fetuses, and LH levels were elevated in PA infants. These studies suggest that experimentally induced fetal androgen excess increases gonadotropin secretion in PA female fetuses and infants and elevates endogenous androgen levels in PA infants. Thus, in this nonhuman primate model, differential programming of the fetal hypothalamo-pituitary unit with concomitant hyperandrogenism provides evidence to suggest developmental origins of LH and androgen excess in adulthood.

Original languageEnglish (US)
Pages (from-to)154-163
Number of pages10
JournalBiology of Reproduction
Issue number1
StatePublished - Jul 2008


  • Androgen excess
  • Early development
  • Environment
  • Fetal programming
  • LH hypersecretion
  • LH negative feedback
  • Luteinizing hormone
  • Testosterone

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology


Dive into the research topics of 'Endocrine antecedents of polycystic ovary syndrome in fetal and infant prenatally androgenized female rhesus monkeys'. Together they form a unique fingerprint.

Cite this