Endo-β-N-acetylglucosaminidases from infant gut-associated bifidobacteria release complex N-glycans from human milk glycoproteins

Daniel Garrido, Charles Nwosu, Santiago Ruiz-Moyano, Danielle Aldredge, J. Bruce German, Carlito B Lebrilla, David A. Mills

Research output: Contribution to journalArticle

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Abstract

Breastfeeding is one of the main factors guiding the composition of the infant gut microbiota in the first months of life. This process is shaped in part by the high amounts of human milk oligosaccharides that serve as a carbon source for saccharolytic bacteria such as Bifidobacterium species. Infant-borne bifidobacteria have developed various molecular strategies for utilizing these oligosaccharides as a carbon source. We hypothesized that these species also interact with N-glycans found in host glycoproteins that are structurally similar to free oligosaccharides in human milk. Endo-β-N- acetylglucosaminidases were identified in certain isolates of Bifidobacterium longum subsp. longum, B. longum subsp. infantis, and Bifidobacterium breve, and their presence correlated with the ability of these strains to deglycosylate glycoproteins. An endoglycosidase from B. infantis ATCC 15697, EndoBI-1, was active toward all major types of N-linked glycans found in glycosylated proteins. Its activity was not affected by core fucosylation or extensive fucosylation, antenna number, or sialylation, releasing several N-glycans from human lactoferrin and immunoglobulins A and G. Extensive N-deglycosylation of whole breast milk was also observed after coincubation with this enzyme. Mutation of the active site of EndoBI-1 did not abolish binding to N-glycosylated proteins, and this mutant specifically recognized Man 3GlcNAc2(α1-6Fuc), the core structure of human N-glycans. EndoBI-1 is constitutively expressed in B. infantis, and incubation of the bacterium with human or bovine lactoferrin led to the induction of genes associated to import and consumption of human milk oligosaccharides, suggesting linked regulatory mechanisms among these glycans. This work reveals an unprecedented interaction of bifidobacteria with host N-glycans and describes a novel endoglycosidase with broad specificity on diverse N-glycan types, potentially a useful tool for glycoproteomics studies.

Original languageEnglish (US)
Pages (from-to)775-785
Number of pages11
JournalMolecular and Cellular Proteomics
Volume11
Issue number9
DOIs
StatePublished - Sep 2012

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Acetylglucosaminidase
Bifidobacterium
Human Milk
Polysaccharides
Glycoproteins
Oligosaccharides
Lactoferrin
Glycoside Hydrolases
Bacteria
Carbon
Sulfalene
Aptitude
Mutant Proteins
endo-alpha-sialidase
Breast Feeding
Immunoglobulin A
Catalytic Domain
Immunoglobulin G
Genes
Antennas

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Cite this

Endo-β-N-acetylglucosaminidases from infant gut-associated bifidobacteria release complex N-glycans from human milk glycoproteins. / Garrido, Daniel; Nwosu, Charles; Ruiz-Moyano, Santiago; Aldredge, Danielle; German, J. Bruce; Lebrilla, Carlito B; Mills, David A.

In: Molecular and Cellular Proteomics, Vol. 11, No. 9, 09.2012, p. 775-785.

Research output: Contribution to journalArticle

Garrido, Daniel ; Nwosu, Charles ; Ruiz-Moyano, Santiago ; Aldredge, Danielle ; German, J. Bruce ; Lebrilla, Carlito B ; Mills, David A. / Endo-β-N-acetylglucosaminidases from infant gut-associated bifidobacteria release complex N-glycans from human milk glycoproteins. In: Molecular and Cellular Proteomics. 2012 ; Vol. 11, No. 9. pp. 775-785.
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