Encapsulation and delivery of plasmid DNA by virus-like nanoparticles engineered from Macrobrachium rosenbergii nodavirus

Pitchanee Jariyapong, Charoonroj Chotwiwatthanakun, Monsicha Somrit, Sarawut Jitrapakdee, Li Xing, Holland R. Cheng, Wattana Weerachatyanukul

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Virus-like particles (VLPs) are potential candidates in developing biological containers for packaging therapeutic or biologically active agents. Here, we expressed Macrobrachium rosenbergii nodavirus (MrNv) capsid protein (encoding amino acids M1-N371 with 6 histidine residuals) in an Escherichia coli BL21(DE3). These easily purified capsid protein self-assembled into VLPs, and disassembly/reassembly could be controlled in a calcium-dependent manner. Physically, MrNv VLPs resisted to digestive enzymes, a property that should be advantageous for protection of active compounds against harsh conditions. We also proved that MrNv VLPs were capable of encapsulating plasmid DNA in the range of 0.035-0.042. mol ratio (DNA/protein) or 2-3 plasmids/VLP (assuming that MrNV VLPs is T= 1, i made up of 60 capsid monomers). These VLPs interacted with cultured insect cells and delivered loaded plasmid DNA into the cells as shown by green fluorescent protein (GFP) reporter. With many advantageous properties including self-encapsulation, MrNv VLPs are good candidates for delivery of therapeutic agents.

Original languageEnglish (US)
Pages (from-to)140-146
Number of pages7
JournalVirus Research
Volume179
Issue number1
DOIs
StatePublished - Jan 22 2014

Keywords

  • Biological container
  • Encapsulation
  • Macrobrachium rosenbergii nodavirus
  • Virus-like particle

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

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