Employment of tandem mass spectrometry for the accurate and specific identification of oligosaccharide structures

Shuai Wu, Juli Salcedo, Ning Tang, Keith Waddell, Rudolf Grimm, J. Bruce German, Carlito B Lebrilla

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

A method is described for the rapid identification of oligosaccharides employing a library of tandem MS spectra. Identification is aided by software that compares the sample tandem MS to those in the library. The method incorporates quadrupole time-of-flight mass spectrometry along with an annotated oligosaccharide (OS) structure library and the MassHunter Personal Compound Database and Library (PCDL) software. With an automated spectra search, OS structures in different samples are readily identified. This method is shown to be useful in the study of milk oligosaccharides but can be readily applied to oligosaccharide pools in other biological tissues.

Original languageEnglish (US)
Pages (from-to)7456-7462
Number of pages7
JournalAnalytical Chemistry
Volume84
Issue number17
DOIs
StatePublished - Sep 4 2012

ASJC Scopus subject areas

  • Analytical Chemistry

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