Emperipolesis Mediated by CD8 T Cells Is a Characteristic Histopathologic Feature of Autoimmune Hepatitis

Qi Miao, Zhaolian Bian, Ruqi Tang, Haiyan Zhang, Qixia Wang, Shanshan Huang, Xiao Xiao, Li Shen, Dekai Qiu, Edward L. Krawitt, M. Eric Gershwin, Xiong Ma

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Emperipolesis has been widely described in patients with autoimmune hepatitis, but the significance and the diagnostic value have not been quantitated. The goal of this study was to define the features and clinical significance of emperipolesis in autoimmune hepatitis (AIH). A retrospective histological evaluation of 101 patients with AIH and 184 controls was performed. Confocal staining for CD4, CD8, CD19, CD56, CD163, and CD11b, CK8/18 and cleaved caspase-3 was performed. Emperipolesis was observed in 65.3 % of the patients with AIH in haematoxylin and eosin (H&E)-stained slides, which was significantly higher than in patients with primary biliary cirrhosis (17.9 %), chronic hepatitis B (14.9 %), and drug-induced liver injury (25.6 %). Among AIH patients, the patients with emperipolesis had significantly higher serum (alanine aminotransferase/aspartate aminotransferase [ALT/AST]) levels. Histologically, emperipolesis was associated with more severe necroinflammatory features and more advanced fibrosis. The lymphocytes in hepatocytes were predominantly as CD8 T cells. Emperipolesis of CD8 T cells induced cleaved caspase-3 expression, and was prominent in areas apoptosis. Emperipolesis is a characteristic feature of AIH which is often seen in conjunction with interface hepatitis, plasmacytic infiltration and hepatocyte rosetting and is associated with more severe necroinflammatory and fibrotic changes. In AIH, emperipolesis is predominantly mediated by CD8 T cells, appears to induce apoptosis and may be another mechanism of autoimmune-mediated hepatocyte injury.

Original languageEnglish (US)
Pages (from-to)226-235
Number of pages10
JournalClinical Reviews in Allergy and Immunology
Volume48
Issue number2-3
DOIs
StatePublished - Jun 1 2015

Fingerprint

Emperipolesis
Autoimmune Hepatitis
T-Lymphocytes
Hepatocytes
Caspase 3
Apoptosis
Chemical and Drug Induced Liver Injury
Biliary Liver Cirrhosis
Chronic Hepatitis B
Hematoxylin
Eosine Yellowish-(YS)
Aspartate Aminotransferases
Alanine Transaminase
Hepatitis
Fibrosis
Lymphocytes
Staining and Labeling

Keywords

  • Autoimmune hepatitis
  • Autoimmune liver disease
  • Chronic hepatitis
  • Emperipolesis
  • Hepatocyte injury
  • Inflammation

ASJC Scopus subject areas

  • Immunology and Allergy

Cite this

Emperipolesis Mediated by CD8 T Cells Is a Characteristic Histopathologic Feature of Autoimmune Hepatitis. / Miao, Qi; Bian, Zhaolian; Tang, Ruqi; Zhang, Haiyan; Wang, Qixia; Huang, Shanshan; Xiao, Xiao; Shen, Li; Qiu, Dekai; Krawitt, Edward L.; Gershwin, M. Eric; Ma, Xiong.

In: Clinical Reviews in Allergy and Immunology, Vol. 48, No. 2-3, 01.06.2015, p. 226-235.

Research output: Contribution to journalArticle

Miao, Q, Bian, Z, Tang, R, Zhang, H, Wang, Q, Huang, S, Xiao, X, Shen, L, Qiu, D, Krawitt, EL, Gershwin, ME & Ma, X 2015, 'Emperipolesis Mediated by CD8 T Cells Is a Characteristic Histopathologic Feature of Autoimmune Hepatitis', Clinical Reviews in Allergy and Immunology, vol. 48, no. 2-3, pp. 226-235. https://doi.org/10.1007/s12016-014-8432-0
Miao, Qi ; Bian, Zhaolian ; Tang, Ruqi ; Zhang, Haiyan ; Wang, Qixia ; Huang, Shanshan ; Xiao, Xiao ; Shen, Li ; Qiu, Dekai ; Krawitt, Edward L. ; Gershwin, M. Eric ; Ma, Xiong. / Emperipolesis Mediated by CD8 T Cells Is a Characteristic Histopathologic Feature of Autoimmune Hepatitis. In: Clinical Reviews in Allergy and Immunology. 2015 ; Vol. 48, No. 2-3. pp. 226-235.
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AB - Emperipolesis has been widely described in patients with autoimmune hepatitis, but the significance and the diagnostic value have not been quantitated. The goal of this study was to define the features and clinical significance of emperipolesis in autoimmune hepatitis (AIH). A retrospective histological evaluation of 101 patients with AIH and 184 controls was performed. Confocal staining for CD4, CD8, CD19, CD56, CD163, and CD11b, CK8/18 and cleaved caspase-3 was performed. Emperipolesis was observed in 65.3 % of the patients with AIH in haematoxylin and eosin (H&E)-stained slides, which was significantly higher than in patients with primary biliary cirrhosis (17.9 %), chronic hepatitis B (14.9 %), and drug-induced liver injury (25.6 %). Among AIH patients, the patients with emperipolesis had significantly higher serum (alanine aminotransferase/aspartate aminotransferase [ALT/AST]) levels. Histologically, emperipolesis was associated with more severe necroinflammatory features and more advanced fibrosis. The lymphocytes in hepatocytes were predominantly as CD8 T cells. Emperipolesis of CD8 T cells induced cleaved caspase-3 expression, and was prominent in areas apoptosis. Emperipolesis is a characteristic feature of AIH which is often seen in conjunction with interface hepatitis, plasmacytic infiltration and hepatocyte rosetting and is associated with more severe necroinflammatory and fibrotic changes. In AIH, emperipolesis is predominantly mediated by CD8 T cells, appears to induce apoptosis and may be another mechanism of autoimmune-mediated hepatocyte injury.

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