Emergence of simian immunodeficiency virus-specific cytotoxic CD4 + T cells and increased humoral responses correlate with control of rebounding viremia in CD8-depleted macaques infected with Rev-independent live-attenuated simian immunodeficiency virus

Agneta Von Gegerfelt, Antonio Valentin, Candido Alicea, Koen K.A. Van Rompay, Marta Marthas, David C. Montefiori, George N. Pavlakis, Barbara K. Felber

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Indian rhesus macaques infected with the Rev-independent live-attenuated SIVmac239 strains control viremia to undetectable levels, have persistent but low cellular and humoral anti-SIV responses, and show no signs of immune deficiency. To analyze the immune mechanisms responsible for viral control, five macaques infected at day 1 after birth were subjected to CD8+ cell depletion at 6.7 y postinfection. This resulted in viremia increases to 3.7-5.5 log10 RNA copies, supporting a role of CD8-mediated responses in the control of viral replication. The rebounding viremia was rapidly controlled to levels below the threshold of detection, and occurred in the absence of SIV-specific CD8+ T cells and significant CD8+ T cell recovery in four of the five animals, suggesting that other mechanisms are involved in the immunological control of viremia. Monitoring immune responses at the time of viral control demonstrated a burst of circulating SIV-specific CD4+ T cells characterized as CD45RA-CD28 +CD95+CCR7- and also granzyme B+, suggesting cytotoxic ability. Control of viremia was also concomitant with increases in humoral responses to Gag and Env, including a transient increase in neutralizing Abs against the neutralization-resistant SIVmac239 in four of five animals. These data demonstrate that a combination of cellular responses mediated by CD4+ T cells and humoral responses was associated with the rapid control of the rebounding viremia in macaques infected by the Rev-independent live-attenuated SIV, even in the absence of measurable SIV-specific CD8+ T cells in the blood, emphasizing the importance of different components of the immune response for full control of SIV infection.

Original languageEnglish (US)
Pages (from-to)3348-3358
Number of pages11
JournalJournal of Immunology
Volume185
Issue number6
DOIs
StatePublished - Sep 15 2010

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Simian Immunodeficiency Virus
Viremia
Macaca
T-Lymphocytes
Immunologic Monitoring
Granzymes
Infection Control
Macaca mulatta
Parturition
RNA

ASJC Scopus subject areas

  • Immunology

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Emergence of simian immunodeficiency virus-specific cytotoxic CD4 + T cells and increased humoral responses correlate with control of rebounding viremia in CD8-depleted macaques infected with Rev-independent live-attenuated simian immunodeficiency virus. / Von Gegerfelt, Agneta; Valentin, Antonio; Alicea, Candido; Van Rompay, Koen K.A.; Marthas, Marta; Montefiori, David C.; Pavlakis, George N.; Felber, Barbara K.

In: Journal of Immunology, Vol. 185, No. 6, 15.09.2010, p. 3348-3358.

Research output: Contribution to journalArticle

Von Gegerfelt, Agneta ; Valentin, Antonio ; Alicea, Candido ; Van Rompay, Koen K.A. ; Marthas, Marta ; Montefiori, David C. ; Pavlakis, George N. ; Felber, Barbara K. / Emergence of simian immunodeficiency virus-specific cytotoxic CD4 + T cells and increased humoral responses correlate with control of rebounding viremia in CD8-depleted macaques infected with Rev-independent live-attenuated simian immunodeficiency virus. In: Journal of Immunology. 2010 ; Vol. 185, No. 6. pp. 3348-3358.
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AU - Valentin, Antonio

AU - Alicea, Candido

AU - Van Rompay, Koen K.A.

AU - Marthas, Marta

AU - Montefiori, David C.

AU - Pavlakis, George N.

AU - Felber, Barbara K.

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