TY - JOUR
T1 - Emergence and transmission of arbovirus evolutionary intermediates with epidemic potential
AU - Stapleford, Kenneth A.
AU - Schneider, Lark L
AU - Lay, Sreyrath
AU - Bordería, Antonio V.
AU - Duong, Veasna
AU - Isakov, Ofer
AU - Rozen-Gagnon, Kathryn
AU - Arias-Goeta, Camilo
AU - Blanc, Hervé
AU - Beaucourt, Stéphanie
AU - Haliloǧlu, Türkan
AU - Schmitt, Christine
AU - Bonne, Isabelle
AU - Ben-Tal, Nir
AU - Shomron, Noam
AU - Failloux, Anna Bella
AU - Buchy, Philippe
AU - Vignuzzi, Marco
PY - 2014/6/11
Y1 - 2014/6/11
N2 - The high replication and mutation rates of RNA viruses can result in the emergence of new epidemic variants. Thus, the ability to follow host-specific evolutionary trajectories of viruses is essential to predict and prevent epidemics. By studying the spatial and temporal evolution of chikungunya virus during natural transmission between mosquitoes and mammals, we have identified viral evolutionary intermediates prior to emergence. Analysis of virus populations at anatomical barriers revealed that the mosquito midgut and salivary gland pose population bottlenecks. By focusing on virus subpopulations in the saliva of multiple mosquito strains, we recapitulated the emergence of a recent epidemic strain of chikungunya and identified E1 glycoprotein mutations with potential to emerge in the future. These mutations confer fitness advantages in mosquito and mammalian hosts by altering virion stability and fusogenic activity. Thus, virus evolutionary trajectories can be predicted and studied in the short term before new variants displace currently circulating strains.
AB - The high replication and mutation rates of RNA viruses can result in the emergence of new epidemic variants. Thus, the ability to follow host-specific evolutionary trajectories of viruses is essential to predict and prevent epidemics. By studying the spatial and temporal evolution of chikungunya virus during natural transmission between mosquitoes and mammals, we have identified viral evolutionary intermediates prior to emergence. Analysis of virus populations at anatomical barriers revealed that the mosquito midgut and salivary gland pose population bottlenecks. By focusing on virus subpopulations in the saliva of multiple mosquito strains, we recapitulated the emergence of a recent epidemic strain of chikungunya and identified E1 glycoprotein mutations with potential to emerge in the future. These mutations confer fitness advantages in mosquito and mammalian hosts by altering virion stability and fusogenic activity. Thus, virus evolutionary trajectories can be predicted and studied in the short term before new variants displace currently circulating strains.
UR - http://www.scopus.com/inward/record.url?scp=84902440512&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84902440512&partnerID=8YFLogxK
U2 - 10.1016/j.chom.2014.05.008
DO - 10.1016/j.chom.2014.05.008
M3 - Article
C2 - 24922573
AN - SCOPUS:84902440512
VL - 15
SP - 706
EP - 716
JO - Cell Host and Microbe
JF - Cell Host and Microbe
SN - 1931-3128
IS - 6
ER -