Embryonic MGE Precursor Cells Grafted into Adult Rat Striatum Integrate and Ameliorate Motor Symptoms in 6-OHDA-Lesioned Rats

Veronica Martinez-Cerdeno, Stephen C Noctor, Ana Espinosa, Jeanelle Ariza, Philip Parker, Samantha Orasji, Marcel M. Daadi, Krystof Bankiewicz, Arturo Alvarez-Buylla, Arnold R. Kriegstein

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

We investigated a strategy to ameliorate the motor symptoms of rats that received 6-hydroxydopamine (6-OHDA) lesions, a rodent model of Parkinson's disease, through transplantation of embryonic medial ganglionic eminence (MGE) cells into the striatum. During brain development, embryonic MGE cells migrate into the striatum and neocortex where they mature into GABAergic interneurons and play a key role in establishing the balance between excitation and inhibition. Unlike most other embryonic neurons, MGE cells retain the capacity for migration and integration when transplanted into the postnatal and adult brain. We performed MGE cell transplantation into the basal ganglia of control and 6-OHDA-lesioned rats. Transplanted MGE cells survived, differentiated into GABA+ neurons, integrated into host circuitry, and modifed motor behavior in both lesioned and control rats. Our data suggest that MGE cell transplantation into the striatum is a promising approach to investigate the potential benefits of remodeling basal ganglia circuitry in neurodegenerative diseases.

Original languageEnglish (US)
Pages (from-to)238-250
Number of pages13
JournalCell Stem Cell
Volume6
Issue number3
DOIs
StatePublished - Mar 5 2010

Fingerprint

Oxidopamine
Cell Transplantation
Basal Ganglia
GABAergic Neurons
Neocortex
Brain
Interneurons
Neurodegenerative Diseases
Parkinson Disease
Rodentia
Transplantation
Neurons

Keywords

  • STEMCELL

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Medicine
  • Genetics

Cite this

Embryonic MGE Precursor Cells Grafted into Adult Rat Striatum Integrate and Ameliorate Motor Symptoms in 6-OHDA-Lesioned Rats. / Martinez-Cerdeno, Veronica; Noctor, Stephen C; Espinosa, Ana; Ariza, Jeanelle; Parker, Philip; Orasji, Samantha; Daadi, Marcel M.; Bankiewicz, Krystof; Alvarez-Buylla, Arturo; Kriegstein, Arnold R.

In: Cell Stem Cell, Vol. 6, No. 3, 05.03.2010, p. 238-250.

Research output: Contribution to journalArticle

Martinez-Cerdeno, V, Noctor, SC, Espinosa, A, Ariza, J, Parker, P, Orasji, S, Daadi, MM, Bankiewicz, K, Alvarez-Buylla, A & Kriegstein, AR 2010, 'Embryonic MGE Precursor Cells Grafted into Adult Rat Striatum Integrate and Ameliorate Motor Symptoms in 6-OHDA-Lesioned Rats', Cell Stem Cell, vol. 6, no. 3, pp. 238-250. https://doi.org/10.1016/j.stem.2010.01.004
Martinez-Cerdeno, Veronica ; Noctor, Stephen C ; Espinosa, Ana ; Ariza, Jeanelle ; Parker, Philip ; Orasji, Samantha ; Daadi, Marcel M. ; Bankiewicz, Krystof ; Alvarez-Buylla, Arturo ; Kriegstein, Arnold R. / Embryonic MGE Precursor Cells Grafted into Adult Rat Striatum Integrate and Ameliorate Motor Symptoms in 6-OHDA-Lesioned Rats. In: Cell Stem Cell. 2010 ; Vol. 6, No. 3. pp. 238-250.
@article{6ca75b1ae2de440d820e2b14d18b8871,
title = "Embryonic MGE Precursor Cells Grafted into Adult Rat Striatum Integrate and Ameliorate Motor Symptoms in 6-OHDA-Lesioned Rats",
abstract = "We investigated a strategy to ameliorate the motor symptoms of rats that received 6-hydroxydopamine (6-OHDA) lesions, a rodent model of Parkinson's disease, through transplantation of embryonic medial ganglionic eminence (MGE) cells into the striatum. During brain development, embryonic MGE cells migrate into the striatum and neocortex where they mature into GABAergic interneurons and play a key role in establishing the balance between excitation and inhibition. Unlike most other embryonic neurons, MGE cells retain the capacity for migration and integration when transplanted into the postnatal and adult brain. We performed MGE cell transplantation into the basal ganglia of control and 6-OHDA-lesioned rats. Transplanted MGE cells survived, differentiated into GABA+ neurons, integrated into host circuitry, and modifed motor behavior in both lesioned and control rats. Our data suggest that MGE cell transplantation into the striatum is a promising approach to investigate the potential benefits of remodeling basal ganglia circuitry in neurodegenerative diseases.",
keywords = "STEMCELL",
author = "Veronica Martinez-Cerdeno and Noctor, {Stephen C} and Ana Espinosa and Jeanelle Ariza and Philip Parker and Samantha Orasji and Daadi, {Marcel M.} and Krystof Bankiewicz and Arturo Alvarez-Buylla and Kriegstein, {Arnold R.}",
year = "2010",
month = "3",
day = "5",
doi = "10.1016/j.stem.2010.01.004",
language = "English (US)",
volume = "6",
pages = "238--250",
journal = "Cell Stem Cell",
issn = "1934-5909",
publisher = "Cell Press",
number = "3",

}

TY - JOUR

T1 - Embryonic MGE Precursor Cells Grafted into Adult Rat Striatum Integrate and Ameliorate Motor Symptoms in 6-OHDA-Lesioned Rats

AU - Martinez-Cerdeno, Veronica

AU - Noctor, Stephen C

AU - Espinosa, Ana

AU - Ariza, Jeanelle

AU - Parker, Philip

AU - Orasji, Samantha

AU - Daadi, Marcel M.

AU - Bankiewicz, Krystof

AU - Alvarez-Buylla, Arturo

AU - Kriegstein, Arnold R.

PY - 2010/3/5

Y1 - 2010/3/5

N2 - We investigated a strategy to ameliorate the motor symptoms of rats that received 6-hydroxydopamine (6-OHDA) lesions, a rodent model of Parkinson's disease, through transplantation of embryonic medial ganglionic eminence (MGE) cells into the striatum. During brain development, embryonic MGE cells migrate into the striatum and neocortex where they mature into GABAergic interneurons and play a key role in establishing the balance between excitation and inhibition. Unlike most other embryonic neurons, MGE cells retain the capacity for migration and integration when transplanted into the postnatal and adult brain. We performed MGE cell transplantation into the basal ganglia of control and 6-OHDA-lesioned rats. Transplanted MGE cells survived, differentiated into GABA+ neurons, integrated into host circuitry, and modifed motor behavior in both lesioned and control rats. Our data suggest that MGE cell transplantation into the striatum is a promising approach to investigate the potential benefits of remodeling basal ganglia circuitry in neurodegenerative diseases.

AB - We investigated a strategy to ameliorate the motor symptoms of rats that received 6-hydroxydopamine (6-OHDA) lesions, a rodent model of Parkinson's disease, through transplantation of embryonic medial ganglionic eminence (MGE) cells into the striatum. During brain development, embryonic MGE cells migrate into the striatum and neocortex where they mature into GABAergic interneurons and play a key role in establishing the balance between excitation and inhibition. Unlike most other embryonic neurons, MGE cells retain the capacity for migration and integration when transplanted into the postnatal and adult brain. We performed MGE cell transplantation into the basal ganglia of control and 6-OHDA-lesioned rats. Transplanted MGE cells survived, differentiated into GABA+ neurons, integrated into host circuitry, and modifed motor behavior in both lesioned and control rats. Our data suggest that MGE cell transplantation into the striatum is a promising approach to investigate the potential benefits of remodeling basal ganglia circuitry in neurodegenerative diseases.

KW - STEMCELL

UR - http://www.scopus.com/inward/record.url?scp=77449150622&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77449150622&partnerID=8YFLogxK

U2 - 10.1016/j.stem.2010.01.004

DO - 10.1016/j.stem.2010.01.004

M3 - Article

C2 - 20207227

AN - SCOPUS:77449150622

VL - 6

SP - 238

EP - 250

JO - Cell Stem Cell

JF - Cell Stem Cell

SN - 1934-5909

IS - 3

ER -