Embryonic handedness choice in C. elegans involves the Gα protein GPA-16

Dominique C. Bergmann, Monica Lee, Barbara Robertson, Meng Fu B Tsou, Lesilee S. Rose, William B. Wood

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

The mechanism by which polarity of the left-right (LR) axis is initially established with the correct handedness is not understood for any embryo. C. elegans embryos exhibit LR asymmetry with an invariant handedness that is first apparent at the six-cell stage and persists throughout development. We show here that a strong loss-of-function mutation in a gene originally designated spn-1 affects early spindle orientations and results in near randomization of handedness choice. This mutation interacts genetically with mutations in three par genes that encode localized cortical components. We show that the spn-1 gene encodes the Gα protein GPA-16, which appears to be required for centrosomal association of a Gβ protein. We will henceforth refer to this gene as gpa-16. These results demonstrate for the first time involvement of heterotrimeric G proteins in establishment of embryonic LR asymmetry and suggest how they might act.

Original languageEnglish (US)
Pages (from-to)5731-5740
Number of pages10
JournalDevelopment
Volume130
Issue number23
DOIs
StatePublished - Dec 2003

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Functional Laterality
GTP-Binding Proteins
Mutation
Genes
Embryonic Structures
Heterotrimeric GTP-Binding Proteins
Random Allocation

Keywords

  • Asymmetry
  • C. elegans
  • G protein

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Bergmann, D. C., Lee, M., Robertson, B., Tsou, M. F. B., Rose, L. S., & Wood, W. B. (2003). Embryonic handedness choice in C. elegans involves the Gα protein GPA-16. Development, 130(23), 5731-5740. https://doi.org/10.1242/dev.00839

Embryonic handedness choice in C. elegans involves the Gα protein GPA-16. / Bergmann, Dominique C.; Lee, Monica; Robertson, Barbara; Tsou, Meng Fu B; Rose, Lesilee S.; Wood, William B.

In: Development, Vol. 130, No. 23, 12.2003, p. 5731-5740.

Research output: Contribution to journalArticle

Bergmann, DC, Lee, M, Robertson, B, Tsou, MFB, Rose, LS & Wood, WB 2003, 'Embryonic handedness choice in C. elegans involves the Gα protein GPA-16', Development, vol. 130, no. 23, pp. 5731-5740. https://doi.org/10.1242/dev.00839
Bergmann DC, Lee M, Robertson B, Tsou MFB, Rose LS, Wood WB. Embryonic handedness choice in C. elegans involves the Gα protein GPA-16. Development. 2003 Dec;130(23):5731-5740. https://doi.org/10.1242/dev.00839
Bergmann, Dominique C. ; Lee, Monica ; Robertson, Barbara ; Tsou, Meng Fu B ; Rose, Lesilee S. ; Wood, William B. / Embryonic handedness choice in C. elegans involves the Gα protein GPA-16. In: Development. 2003 ; Vol. 130, No. 23. pp. 5731-5740.
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