Elucidation of clathrin-mediated endocytosis in tetrahymena reveals an evolutionarily convergent recruitment of dynamin

Nels C. Elde, Garry Morgan, Mark Winey, Linda Sperling, Aaron P. Turkewitz

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Ciliates, although single-celled organisms, contain numerous subcellular structures and pathways usually associated with metazoans. How this cell biological complexity relates to the evolution of molecular elements is unclear, because features in these cells have been defined mainly at the morphological level. Among these ciliate features are structures resembling clathrin-coated, endocytic pits associated with plasma membrane invaginations called parasomal sacs. The combination of genome-wide sequencing in Tetrahymena thermophila with tools for gene expression and replacement has allowed us to examine this pathway in detail. Here we demonstrate that parasomal sacs are sites of clathrindependent endocytosis and that AP-2 localizes to these sites. Unexpectedly, endocytosis in Tetrahymena also involves a protein in the dynamin family, Drp1p (Dynamin-related protein 1). While phylogenetic analysis of AP subunits indicates a primitive origin for clathrin-mediated endocytosis, similar analysis of dynamin-related proteins suggests, strikingly, that the recruitment of dynamin-family proteins to the endocytic pathway occurred independently during the course of the ciliate and metazoan radiations. Consistent with this, our functional analysis suggests that the precise roles of dynamins in endocytosis, as well as the mechanisms of targeting, differ in metazoans and ciliates.

Original languageEnglish (US)
Pages (from-to)514-526
Number of pages13
JournalPLoS Genetics
Volume1
Issue number5
DOIs
StatePublished - Jan 1 2005
Externally publishedYes

Fingerprint

Dynamins
Tetrahymena
clathrin
Clathrin
endocytosis
ciliate
Endocytosis
Ciliophora
protein
Proteins
proteins
Tetrahymena thermophila
metazoan
Molecular Evolution
targeting
gene expression
genome
replacement
membrane
phylogenetics

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

Elucidation of clathrin-mediated endocytosis in tetrahymena reveals an evolutionarily convergent recruitment of dynamin. / Elde, Nels C.; Morgan, Garry; Winey, Mark; Sperling, Linda; Turkewitz, Aaron P.

In: PLoS Genetics, Vol. 1, No. 5, 01.01.2005, p. 514-526.

Research output: Contribution to journalArticle

Elde, Nels C. ; Morgan, Garry ; Winey, Mark ; Sperling, Linda ; Turkewitz, Aaron P. / Elucidation of clathrin-mediated endocytosis in tetrahymena reveals an evolutionarily convergent recruitment of dynamin. In: PLoS Genetics. 2005 ; Vol. 1, No. 5. pp. 514-526.
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