Elucidating the genetic basis of antioxidant status in lettuce (Lactuca sativa)

Annabelle Damerum, Stacey L. Selmes, Gaia F. Biggi, Graham J.J. Clarkson, Steve D. Rothwell, Maria José Truco, Richard W Michelmore, Robert D. Hancock, Connie Shellcock, Mark A. Chapman, Gail Taylor

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A diet rich in phytonutrients from fruit and vegetables has been acknowledged to afford protection against a range of human diseases, but many of the most popular vegetables are low in phytonutrients. Wild relatives of crops may contain allelic variation for genes determining the concentrations of these beneficial phytonutrients, and therefore understanding the genetic basis of this variation is important for breeding efforts to enhance nutritional quality. In this study, lettuce recombinant inbred lines, generated from a cross between wild and cultivated lettuce (Lactuca serriola and Lactuca sativa, respectively), were analysed for antioxidant (AO) potential and important phytonutrients including carotenoids, chlorophyll and phenolic compounds. When grown in two environments, 96 quantitative trait loci (QTL) were identified for these nutritional traits: 4 for AO potential, 2 for carotenoid content, 3 for total chlorophyll content and 87 for individual phenolic compounds (two per compound on average). Most often, the L. serriola alleles conferred an increase in total AOs and metabolites. Candidate genes underlying these QTL were identified by BLASTn searches; in several cases, these had functions suggesting involvement in phytonutrient biosynthetic pathways. Analysis of a QTL on linkage group 3, which accounted for .30% of the variation in AO potential, revealed several candidate genes encoding multiple MYB transcription factors which regulate flavonoid biosynthesis and flavanone 3-hydroxylase, an enzyme involved in the biosynthesis of the flavonoids quercetin and kaempferol, which are known to have powerful AO activity. Follow-up quantitative RT-PCR of these candidates revealed that 5 out of 10 genes investigated were significantly differentially expressed between the wild and cultivated parents, providing further evidence of their potential involvement in determining the contrasting phenotypes. These results offer exciting opportunities to improve the nutritional content and health benefits of lettuce through marker-assisted breeding.

Original languageEnglish (US)
Article number15055
JournalHorticulture Research
Volume2
DOIs
StatePublished - 2015

Fingerprint

Lettuce
Phytochemicals
Lactuca sativa
lettuce
Antioxidants
Lactuca serriola
Quantitative Trait Loci
antioxidants
quantitative trait loci
Genes
Biosynthesis
Vegetables
phenolic compounds
Carotenoids
Chlorophyll
carotenoids
flavonoids
genes
Flavonoids
vegetables

ASJC Scopus subject areas

  • Horticulture
  • Plant Science
  • Biochemistry
  • Biotechnology
  • Genetics

Cite this

Damerum, A., Selmes, S. L., Biggi, G. F., Clarkson, G. J. J., Rothwell, S. D., Truco, M. J., ... Taylor, G. (2015). Elucidating the genetic basis of antioxidant status in lettuce (Lactuca sativa). Horticulture Research, 2, [15055]. https://doi.org/10.1038/hortres.2015.55

Elucidating the genetic basis of antioxidant status in lettuce (Lactuca sativa). / Damerum, Annabelle; Selmes, Stacey L.; Biggi, Gaia F.; Clarkson, Graham J.J.; Rothwell, Steve D.; Truco, Maria José; Michelmore, Richard W; Hancock, Robert D.; Shellcock, Connie; Chapman, Mark A.; Taylor, Gail.

In: Horticulture Research, Vol. 2, 15055, 2015.

Research output: Contribution to journalArticle

Damerum, A, Selmes, SL, Biggi, GF, Clarkson, GJJ, Rothwell, SD, Truco, MJ, Michelmore, RW, Hancock, RD, Shellcock, C, Chapman, MA & Taylor, G 2015, 'Elucidating the genetic basis of antioxidant status in lettuce (Lactuca sativa)', Horticulture Research, vol. 2, 15055. https://doi.org/10.1038/hortres.2015.55
Damerum A, Selmes SL, Biggi GF, Clarkson GJJ, Rothwell SD, Truco MJ et al. Elucidating the genetic basis of antioxidant status in lettuce (Lactuca sativa). Horticulture Research. 2015;2. 15055. https://doi.org/10.1038/hortres.2015.55
Damerum, Annabelle ; Selmes, Stacey L. ; Biggi, Gaia F. ; Clarkson, Graham J.J. ; Rothwell, Steve D. ; Truco, Maria José ; Michelmore, Richard W ; Hancock, Robert D. ; Shellcock, Connie ; Chapman, Mark A. ; Taylor, Gail. / Elucidating the genetic basis of antioxidant status in lettuce (Lactuca sativa). In: Horticulture Research. 2015 ; Vol. 2.
@article{572d649e22c64e9791e01f40d5c1f1d2,
title = "Elucidating the genetic basis of antioxidant status in lettuce (Lactuca sativa)",
abstract = "A diet rich in phytonutrients from fruit and vegetables has been acknowledged to afford protection against a range of human diseases, but many of the most popular vegetables are low in phytonutrients. Wild relatives of crops may contain allelic variation for genes determining the concentrations of these beneficial phytonutrients, and therefore understanding the genetic basis of this variation is important for breeding efforts to enhance nutritional quality. In this study, lettuce recombinant inbred lines, generated from a cross between wild and cultivated lettuce (Lactuca serriola and Lactuca sativa, respectively), were analysed for antioxidant (AO) potential and important phytonutrients including carotenoids, chlorophyll and phenolic compounds. When grown in two environments, 96 quantitative trait loci (QTL) were identified for these nutritional traits: 4 for AO potential, 2 for carotenoid content, 3 for total chlorophyll content and 87 for individual phenolic compounds (two per compound on average). Most often, the L. serriola alleles conferred an increase in total AOs and metabolites. Candidate genes underlying these QTL were identified by BLASTn searches; in several cases, these had functions suggesting involvement in phytonutrient biosynthetic pathways. Analysis of a QTL on linkage group 3, which accounted for .30{\%} of the variation in AO potential, revealed several candidate genes encoding multiple MYB transcription factors which regulate flavonoid biosynthesis and flavanone 3-hydroxylase, an enzyme involved in the biosynthesis of the flavonoids quercetin and kaempferol, which are known to have powerful AO activity. Follow-up quantitative RT-PCR of these candidates revealed that 5 out of 10 genes investigated were significantly differentially expressed between the wild and cultivated parents, providing further evidence of their potential involvement in determining the contrasting phenotypes. These results offer exciting opportunities to improve the nutritional content and health benefits of lettuce through marker-assisted breeding.",
author = "Annabelle Damerum and Selmes, {Stacey L.} and Biggi, {Gaia F.} and Clarkson, {Graham J.J.} and Rothwell, {Steve D.} and Truco, {Maria Jos{\'e}} and Michelmore, {Richard W} and Hancock, {Robert D.} and Connie Shellcock and Chapman, {Mark A.} and Gail Taylor",
year = "2015",
doi = "10.1038/hortres.2015.55",
language = "English (US)",
volume = "2",
journal = "Horticulture Research",
issn = "2052-7276",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Elucidating the genetic basis of antioxidant status in lettuce (Lactuca sativa)

AU - Damerum, Annabelle

AU - Selmes, Stacey L.

AU - Biggi, Gaia F.

AU - Clarkson, Graham J.J.

AU - Rothwell, Steve D.

AU - Truco, Maria José

AU - Michelmore, Richard W

AU - Hancock, Robert D.

AU - Shellcock, Connie

AU - Chapman, Mark A.

AU - Taylor, Gail

PY - 2015

Y1 - 2015

N2 - A diet rich in phytonutrients from fruit and vegetables has been acknowledged to afford protection against a range of human diseases, but many of the most popular vegetables are low in phytonutrients. Wild relatives of crops may contain allelic variation for genes determining the concentrations of these beneficial phytonutrients, and therefore understanding the genetic basis of this variation is important for breeding efforts to enhance nutritional quality. In this study, lettuce recombinant inbred lines, generated from a cross between wild and cultivated lettuce (Lactuca serriola and Lactuca sativa, respectively), were analysed for antioxidant (AO) potential and important phytonutrients including carotenoids, chlorophyll and phenolic compounds. When grown in two environments, 96 quantitative trait loci (QTL) were identified for these nutritional traits: 4 for AO potential, 2 for carotenoid content, 3 for total chlorophyll content and 87 for individual phenolic compounds (two per compound on average). Most often, the L. serriola alleles conferred an increase in total AOs and metabolites. Candidate genes underlying these QTL were identified by BLASTn searches; in several cases, these had functions suggesting involvement in phytonutrient biosynthetic pathways. Analysis of a QTL on linkage group 3, which accounted for .30% of the variation in AO potential, revealed several candidate genes encoding multiple MYB transcription factors which regulate flavonoid biosynthesis and flavanone 3-hydroxylase, an enzyme involved in the biosynthesis of the flavonoids quercetin and kaempferol, which are known to have powerful AO activity. Follow-up quantitative RT-PCR of these candidates revealed that 5 out of 10 genes investigated were significantly differentially expressed between the wild and cultivated parents, providing further evidence of their potential involvement in determining the contrasting phenotypes. These results offer exciting opportunities to improve the nutritional content and health benefits of lettuce through marker-assisted breeding.

AB - A diet rich in phytonutrients from fruit and vegetables has been acknowledged to afford protection against a range of human diseases, but many of the most popular vegetables are low in phytonutrients. Wild relatives of crops may contain allelic variation for genes determining the concentrations of these beneficial phytonutrients, and therefore understanding the genetic basis of this variation is important for breeding efforts to enhance nutritional quality. In this study, lettuce recombinant inbred lines, generated from a cross between wild and cultivated lettuce (Lactuca serriola and Lactuca sativa, respectively), were analysed for antioxidant (AO) potential and important phytonutrients including carotenoids, chlorophyll and phenolic compounds. When grown in two environments, 96 quantitative trait loci (QTL) were identified for these nutritional traits: 4 for AO potential, 2 for carotenoid content, 3 for total chlorophyll content and 87 for individual phenolic compounds (two per compound on average). Most often, the L. serriola alleles conferred an increase in total AOs and metabolites. Candidate genes underlying these QTL were identified by BLASTn searches; in several cases, these had functions suggesting involvement in phytonutrient biosynthetic pathways. Analysis of a QTL on linkage group 3, which accounted for .30% of the variation in AO potential, revealed several candidate genes encoding multiple MYB transcription factors which regulate flavonoid biosynthesis and flavanone 3-hydroxylase, an enzyme involved in the biosynthesis of the flavonoids quercetin and kaempferol, which are known to have powerful AO activity. Follow-up quantitative RT-PCR of these candidates revealed that 5 out of 10 genes investigated were significantly differentially expressed between the wild and cultivated parents, providing further evidence of their potential involvement in determining the contrasting phenotypes. These results offer exciting opportunities to improve the nutritional content and health benefits of lettuce through marker-assisted breeding.

UR - http://www.scopus.com/inward/record.url?scp=84994369882&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84994369882&partnerID=8YFLogxK

U2 - 10.1038/hortres.2015.55

DO - 10.1038/hortres.2015.55

M3 - Article

AN - SCOPUS:84994369882

VL - 2

JO - Horticulture Research

JF - Horticulture Research

SN - 2052-7276

M1 - 15055

ER -