Ellipsometry-based biosensor for label-free detection of biomolecular interactions in microarray format

Yung Shin Sun, Xiang Dong Zhu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Microarrays of biological molecules are useful tools for discovery and functionality characterization in fundamental and applied research of genomics, proteomics, glycomics, and cytomics. They provide a high-throughput platform that enables parallel studies of hundreds to tens of thousands of distinct biomolecular reactions. Usually, the characterization of binding reactions between surface-immobilized targets and solutionphase analytes involves fluorescence-based detection methods. However, labeling analytes inevitably changes innate properties of the molecules and, in turn, modifies analyte-target interactions often in an uncharacterized manner. As a result, label-free microarray detection is desirable. In this study, optical microscopes based on the obliqueincidence reflectivity difference (OI-RD) technique are developed and used to detect biomolecular interactions in microarray format. OI-RD, a most sensitive form of optical elliposometry, measures the difference in reflectivity change (both magnitude and phase) between two polarized components of an optical beam. Such a difference is related to the thickness and dielectric constant of surface-immobilized biomolecules. Here, we report the use of such microscopes to study novel protein-protein, oligosaccharide-protein, and small molecule-protein interactions. These experimental results demonstrate that the OI-RD microscopes can serve as powerful tools in biosensing, high-throughput screening, and other applications in biophysics, biochemistry, and biomedical engineering.

Original languageEnglish (US)
Pages (from-to)643-651
Number of pages9
JournalSensors and Materials
Volume25
Issue number9
StatePublished - 2013

Fingerprint

Ellipsometry
Microarrays
bioinstrumentation
Biosensors
ellipsometry
format
Labels
proteins
Proteins
reflectance
Microscopes
Molecules
microscopes
Throughput
interactions
Biophysics
biophysics
molecules
Oligosaccharides
biochemistry

Keywords

  • Biomolecular interaction
  • Biosensor
  • High throughput
  • Label-free detection
  • Microarray
  • Oblique-incidence reflectivity difference

ASJC Scopus subject areas

  • Instrumentation
  • Materials Science(all)

Cite this

Ellipsometry-based biosensor for label-free detection of biomolecular interactions in microarray format. / Sun, Yung Shin; Zhu, Xiang Dong.

In: Sensors and Materials, Vol. 25, No. 9, 2013, p. 643-651.

Research output: Contribution to journalArticle

@article{3b59413065e7435aa454e2ff2bfbcfb7,
title = "Ellipsometry-based biosensor for label-free detection of biomolecular interactions in microarray format",
abstract = "Microarrays of biological molecules are useful tools for discovery and functionality characterization in fundamental and applied research of genomics, proteomics, glycomics, and cytomics. They provide a high-throughput platform that enables parallel studies of hundreds to tens of thousands of distinct biomolecular reactions. Usually, the characterization of binding reactions between surface-immobilized targets and solutionphase analytes involves fluorescence-based detection methods. However, labeling analytes inevitably changes innate properties of the molecules and, in turn, modifies analyte-target interactions often in an uncharacterized manner. As a result, label-free microarray detection is desirable. In this study, optical microscopes based on the obliqueincidence reflectivity difference (OI-RD) technique are developed and used to detect biomolecular interactions in microarray format. OI-RD, a most sensitive form of optical elliposometry, measures the difference in reflectivity change (both magnitude and phase) between two polarized components of an optical beam. Such a difference is related to the thickness and dielectric constant of surface-immobilized biomolecules. Here, we report the use of such microscopes to study novel protein-protein, oligosaccharide-protein, and small molecule-protein interactions. These experimental results demonstrate that the OI-RD microscopes can serve as powerful tools in biosensing, high-throughput screening, and other applications in biophysics, biochemistry, and biomedical engineering.",
keywords = "Biomolecular interaction, Biosensor, High throughput, Label-free detection, Microarray, Oblique-incidence reflectivity difference",
author = "Sun, {Yung Shin} and Zhu, {Xiang Dong}",
year = "2013",
language = "English (US)",
volume = "25",
pages = "643--651",
journal = "Sensors and Materials",
issn = "0914-4935",
publisher = "M Y U Scientific Publishing Division",
number = "9",

}

TY - JOUR

T1 - Ellipsometry-based biosensor for label-free detection of biomolecular interactions in microarray format

AU - Sun, Yung Shin

AU - Zhu, Xiang Dong

PY - 2013

Y1 - 2013

N2 - Microarrays of biological molecules are useful tools for discovery and functionality characterization in fundamental and applied research of genomics, proteomics, glycomics, and cytomics. They provide a high-throughput platform that enables parallel studies of hundreds to tens of thousands of distinct biomolecular reactions. Usually, the characterization of binding reactions between surface-immobilized targets and solutionphase analytes involves fluorescence-based detection methods. However, labeling analytes inevitably changes innate properties of the molecules and, in turn, modifies analyte-target interactions often in an uncharacterized manner. As a result, label-free microarray detection is desirable. In this study, optical microscopes based on the obliqueincidence reflectivity difference (OI-RD) technique are developed and used to detect biomolecular interactions in microarray format. OI-RD, a most sensitive form of optical elliposometry, measures the difference in reflectivity change (both magnitude and phase) between two polarized components of an optical beam. Such a difference is related to the thickness and dielectric constant of surface-immobilized biomolecules. Here, we report the use of such microscopes to study novel protein-protein, oligosaccharide-protein, and small molecule-protein interactions. These experimental results demonstrate that the OI-RD microscopes can serve as powerful tools in biosensing, high-throughput screening, and other applications in biophysics, biochemistry, and biomedical engineering.

AB - Microarrays of biological molecules are useful tools for discovery and functionality characterization in fundamental and applied research of genomics, proteomics, glycomics, and cytomics. They provide a high-throughput platform that enables parallel studies of hundreds to tens of thousands of distinct biomolecular reactions. Usually, the characterization of binding reactions between surface-immobilized targets and solutionphase analytes involves fluorescence-based detection methods. However, labeling analytes inevitably changes innate properties of the molecules and, in turn, modifies analyte-target interactions often in an uncharacterized manner. As a result, label-free microarray detection is desirable. In this study, optical microscopes based on the obliqueincidence reflectivity difference (OI-RD) technique are developed and used to detect biomolecular interactions in microarray format. OI-RD, a most sensitive form of optical elliposometry, measures the difference in reflectivity change (both magnitude and phase) between two polarized components of an optical beam. Such a difference is related to the thickness and dielectric constant of surface-immobilized biomolecules. Here, we report the use of such microscopes to study novel protein-protein, oligosaccharide-protein, and small molecule-protein interactions. These experimental results demonstrate that the OI-RD microscopes can serve as powerful tools in biosensing, high-throughput screening, and other applications in biophysics, biochemistry, and biomedical engineering.

KW - Biomolecular interaction

KW - Biosensor

KW - High throughput

KW - Label-free detection

KW - Microarray

KW - Oblique-incidence reflectivity difference

UR - http://www.scopus.com/inward/record.url?scp=84892179468&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84892179468&partnerID=8YFLogxK

M3 - Article

VL - 25

SP - 643

EP - 651

JO - Sensors and Materials

JF - Sensors and Materials

SN - 0914-4935

IS - 9

ER -