ELISA for the measurement of serum and urinary chorionic gonadotropin concentrations in the laboratory macaque

C. J. Munro, L. S. Laughlin, J. C. Illera, J. Dieter, Andrew G Hendrickx, B. L. Lasley

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A rapid, sensitive, enzyme-linked immunosorbant assay (ELISA) for the measurement of chorionic gonadotropin (CG) in serum and urine samples of laboratory macaques is reported. The ligand (CG) is captured by a readily available, widely used, and well-characterized monoclonal antibody (Mab, 518B7) generated against the β subunit of bovine luteinizing hormone (LH). This Mab, while specific for LH, shows very little species specificity, and has been shown to detect LH and CG by radioimmunoassay (RIA) in both human and non-human primates. A polyclonal antiserum raised in rabbits against human chorionic gonadotropin (hCG) is conjugated to horseradish peroxidase, and is used as the second antibody signal. This anti-hCG antiserum cross reacts with CG secreted by both the human (hCG) and macaque (mCG). The ELISA utilizes hCG as the standard, and results are based on the relative concentrations of mCG in serum and urine. Total assay time is less than 5 hours. Range of the standard curve is 0.002 to 0.5 ng hCG/well, and the least detectable concentration of hCG is 0.0023 ± 0.0007 ng/well. Pregnancy was detected in early pregnant macaques (M. fascicularis) on 9 (N = 1/16), 10 (N = 1/16), 11 (N = 1/16), 12 (N = 6/16), 13 (N = 1/16), 14 (N = 4/16), and 15 (N = 2/16) days following the pre-ovulatory urinary estrone conjugate peak. The detection of pregnancy by urinary mCG occurred approximately 24 to 72 hours after its detection in serum.

Original languageEnglish (US)
Pages (from-to)307-322
Number of pages16
JournalAmerican Journal of Primatology
Volume41
Issue number4
DOIs
StatePublished - 1997

Fingerprint

chorionic gonadotropins
human chorionic gonadotropin
blood serum
Macaca
hormone
serum
assay
enzyme
pregnancy
urine
antibody
assays
luteinizing hormone
enzymes
primate
ligand
antiserum
estrone
pregnancy diagnosis
radioimmunoassays

Keywords

  • ELISA
  • enzyme immunoassay
  • mCG
  • monkey chorionic gonadotropin

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

ELISA for the measurement of serum and urinary chorionic gonadotropin concentrations in the laboratory macaque. / Munro, C. J.; Laughlin, L. S.; Illera, J. C.; Dieter, J.; Hendrickx, Andrew G; Lasley, B. L.

In: American Journal of Primatology, Vol. 41, No. 4, 1997, p. 307-322.

Research output: Contribution to journalArticle

Munro, C. J. ; Laughlin, L. S. ; Illera, J. C. ; Dieter, J. ; Hendrickx, Andrew G ; Lasley, B. L. / ELISA for the measurement of serum and urinary chorionic gonadotropin concentrations in the laboratory macaque. In: American Journal of Primatology. 1997 ; Vol. 41, No. 4. pp. 307-322.
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