ELISA as an affordable methodology for monitoring groundwater contamination by pesticides in low-income countries

Beatriz M. Brena, Lourdes Arellano, Caterina Rufo, Michael S. Last, Jorge Montaño, Eduardo Egaña Cerni, Gualberto Gonzalez-Sapienza, Jerold A Last

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

The traditional instrumental technology for pesticide residue analysis is too expensive and labor-intense to meet the regional needs concerning environmental monitoring. ELISA methodology was used for a pilot scale study of ground water quality in an agricultural region a few kilometers southwest of Montevideo, the capital city of Uruguay. The study spanned 2 years and examined concentrations (detection limits are given in [ppb]) of two triazine herbicides (simazine [0.3] and atrazine [0.4]) and the carbamate insecticide carbaryl [10] and its major metabolite 1-naphthol [17]. In general, pesticide concentrations were below detection limits in the samples tested and in all cases were well below the maximum contaminant levels set by the U.S. EPA. 1-Naphthol was detected frequently by ELISA, but the assay may have tended to systematically overestimate this analyte. To our knowledge, this is the first study of its type in Uruguay and perhaps the first systematic approach to monitoring for organic pesticides in groundwater water sources in the temperate region of South America.

Original languageEnglish (US)
Pages (from-to)3896-3903
Number of pages8
JournalEnvironmental Science and Technology
Volume39
Issue number11
DOIs
StatePublished - Jun 1 2005

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Science(all)
  • Environmental Chemistry

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    Brena, B. M., Arellano, L., Rufo, C., Last, M. S., Montaño, J., Cerni, E. E., Gonzalez-Sapienza, G., & Last, J. A. (2005). ELISA as an affordable methodology for monitoring groundwater contamination by pesticides in low-income countries. Environmental Science and Technology, 39(11), 3896-3903. https://doi.org/10.1021/es048620d