Abstract
Although recombinant outer surface protein A (OspA) of Borrelia burgdorferi protects mice against injected Lyme disease spirochetes, the mode of protection has not yet been explored. Indeed, the efficacy of vaccine-induced immunity against a realistic vector-mediated challenge remains unexplored. Accordingly, we determined whether this immunogen protects mice against spirochetes delivered by nymphal Ixodes dammini ticks. Following challenge by tick bite, no spirochetes could be cultured from immunized mice, and no characteristic histopathology was found. The spirochete was not detected in ticks that fed on immunized animals and was present in virtually all ticks that fed on nonimmunized mice. We conclude that OspA-immunized mice are protected from spirochetal infection, at least in part, because the spirochete is destroyed in the infecting tick.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 5418-5421 |
| Number of pages | 4 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 89 |
| Issue number | 12 |
| State | Published - 1992 |
| Externally published | Yes |
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Keywords
- Ixodes dammini
- Lyme disease
- Spirochete
- Vaccine development
ASJC Scopus subject areas
- Genetics
- General
Cite this
Elimination of Borrelia burgdorferi from vector ticks feeding on OspA-immunized mice. / Fikrig, Erol; Telford, Sam R.; Barthold, Stephen W; Kantor, Fred S.; Spielman, Andrew; Flavell, Richard A.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 12, 1992, p. 5418-5421.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Elimination of Borrelia burgdorferi from vector ticks feeding on OspA-immunized mice
AU - Fikrig, Erol
AU - Telford, Sam R.
AU - Barthold, Stephen W
AU - Kantor, Fred S.
AU - Spielman, Andrew
AU - Flavell, Richard A.
PY - 1992
Y1 - 1992
N2 - Although recombinant outer surface protein A (OspA) of Borrelia burgdorferi protects mice against injected Lyme disease spirochetes, the mode of protection has not yet been explored. Indeed, the efficacy of vaccine-induced immunity against a realistic vector-mediated challenge remains unexplored. Accordingly, we determined whether this immunogen protects mice against spirochetes delivered by nymphal Ixodes dammini ticks. Following challenge by tick bite, no spirochetes could be cultured from immunized mice, and no characteristic histopathology was found. The spirochete was not detected in ticks that fed on immunized animals and was present in virtually all ticks that fed on nonimmunized mice. We conclude that OspA-immunized mice are protected from spirochetal infection, at least in part, because the spirochete is destroyed in the infecting tick.
AB - Although recombinant outer surface protein A (OspA) of Borrelia burgdorferi protects mice against injected Lyme disease spirochetes, the mode of protection has not yet been explored. Indeed, the efficacy of vaccine-induced immunity against a realistic vector-mediated challenge remains unexplored. Accordingly, we determined whether this immunogen protects mice against spirochetes delivered by nymphal Ixodes dammini ticks. Following challenge by tick bite, no spirochetes could be cultured from immunized mice, and no characteristic histopathology was found. The spirochete was not detected in ticks that fed on immunized animals and was present in virtually all ticks that fed on nonimmunized mice. We conclude that OspA-immunized mice are protected from spirochetal infection, at least in part, because the spirochete is destroyed in the infecting tick.
KW - Ixodes dammini
KW - Lyme disease
KW - Spirochete
KW - Vaccine development
UR - http://www.scopus.com/inward/record.url?scp=0026764138&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026764138&partnerID=8YFLogxK
M3 - Article
C2 - 1608951
AN - SCOPUS:0026764138
VL - 89
SP - 5418
EP - 5421
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 12
ER -