Elevated serum levels of mucin-associated antigen in patients with acute respiratory distress syndrome

Jin Yuan Shih, Shuenn Chen Yang, Chong Jen Yu, Heuy Dong Wu, Yuang Shuang Liaw, Reen Wu, Pan Chyr Yang

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Increased serum levels of mucin-associated antigen have been previously demonstrated in patients with cystic fibrosis (CF) and interstitial pneumonia, and in lung-transplant recipients. The present study assessed the serum airway mucin levels in patients with acute respiratory distress syndrome (ARDS). An enzyme-linked immunosorbent assay (ELISA) method with a human-airway-mucin-specific monoclonal antibody (17Q2) was used to measure serum mucin levels in normal subjects, chronic smokers, patients with chronic bronchitis and other pulmonary diseases, patients with acute cardiogenic lung edema, and patients with ARDS. The serum mucin levels measured 9.9 ± 0.8 ng/ml (mean ± SEM, n = 59) in normal subjects, 12.7 ± 1.6 ng/ml (n = 29) in chronic smokers, 21.8 ± 1.9 ng/ml (n = 28) in patients with chronic bronchitis and other pulmonary diseases, 9.0 ± 3.1 ng/ml (n = 5) in patients with acute cardiogenic lung edema. The serum mucin level was 53.8 ± 6.6 ng/ml (n = 13) in patients with ARDS (p < 0.05, as compared with the four other groups). Serial measurements of serum mucin levels were obtained in patients with ARDS. Statistical analysis showed an inverse correlation of serial measurements of serum mucin with static respiratory-system compliance (p = 0.021), an inverse correlation of sequential serum mucin levels and log(Pao2/Fio2) (p = 0.016), and a positive correlation of sequential serum mucin levels and lung injury score (LIS) (p = 0.019). Gel-filtration analysis showed that mucin-associated antigens in ARDS sera were polydispersed and smaller than the antigens in normal sera. This study indicates that an increasing amount of degraded mucin occurs in patients with ARDS.

Original languageEnglish (US)
Pages (from-to)1453-1457
Number of pages5
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume156
Issue number5
StatePublished - 1997

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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